Trehalose Delays Senescence in Cut Gladiolus Spikes

Otsubo, Mayuko; Iwaya‐Inoue, Mari

doi:10.21273/hortsci.35.6.1107

Cited by 32 publications

(27 citation statements)

References 26 publications

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“…S1). These results were consistent with the suggestion that trehalose may play a role in promoting water retention in cut flowers (Otsubo and Iwaya-Inoue, 2000).…”

Section: Effects Of Trehalose and Validamycin A On Quality Of Infloresupporting

confidence: 92%

“…Trehalose treatment also suppressed water loss ( Fig. S1) and enhanced viability in petals, although slight wilting of the bracts of cut gladiolus has been observed (Otsubo and Iwaya-Inoue, 2000). Yamada et al (2003) reported that trehalose has a suppressive effect on apoptotic cell death.…”

Section: Roles Of Sucrose and Trehalosementioning

confidence: 88%

“…Trehalose is found in bacteria, yeast, fungi, insects, and invertebrate animals but is only rarely present in higher plants (Müller et al, 1995b). However, trehalose prolonged the vase life of cut flowers of several species, including the gladiolus (Otsubo and Iwaya-Inoue, 2000;Yamada et al, 2003;Yamane et al, 2005) and tulip (Iwaya-Inoue and Takata, 2001). Trehalose maintains turgor in petal tissues of cut tulip flowers (Iwaya-Inoue and Takata, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Effects of Trehalose and Sucrose on the Vase Life and Physiology of Cut Astilbe (Astilbe × arendsii Arends) Flowers

et al. 2019

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The postharvest physiology of cut astilbe inflorescences (Astilbe × arendsii), which consist of many small florets with a short vase life, was studied in response to treatments to extend their vase life. Exogenous ethylene treatment at 0.3 μL•L −1 for 7 h did not affect the senescence of inflorescences or leaves of five cultivars and 0.2 mM silver thiosulfate for 2 h did not improve the quality of inflorescences of three cultivars, which indicated that ethylene is not a critical factor for senescence in astilbe florets. Continuous treatment with 2% sucrose or 2% trehalose solutions prolonged the cut inflorescence vase life of one or two of five astilbe cultivars, respectively. Pulse treatment with 2% trehalose in combination with 6% sucrose increased total soluble sugar contents from 11.4 to 57.6 mg•g −1 FW and raised the respiration rate of inflorescences from 15.3 to 28.4 μmol CO 2 •h −1 •g −1 FW at 2 days after harvest (DAH) in the cultivar 'Gloria Purpurea'. However, the effects of pulse treatment diminished at 4 DAH and extended the vase life from 4.0 days to a maximum of 5.6 days, which suggested that pulse treatments were inadequate to maintain sugar contents and respiration activity. Continuous treatment with 6% sucrose extended the vase life from 4.3 to 10.0 days and raised the chroma (C*) value of florets from 28.7 to 54.9 at 8 DAH. Continuous treatment with 2% trehalase + 4% sucrose markedly prolonged the vase life to 11.5 days and increased the C* value to 53.9 at 8 DAH. Under stereomicroscopic observation, continuous treatment with 2% trehalase + 4% sucrose maintained more vivid pink color of petals, styles, filaments and receptacles than those in control florets at 9 DAH. Combined treatment with 2% trehalose and 30 μM validamycin A, a potent inhibitor of trehalose metabolizing activity, induced severe wilting of florets and necrotic spots on leaves. Exogenous trehalose may be hydrolyzed by trehalose metabolizing activity in cut astilbe inflorescences. The results suggest that continuous treatment with trehalose and sucrose solutions is effective to maintain development and delay senescence of florets to extend the vase life of cut astilbe inflorescences.

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“…S1). These results were consistent with the suggestion that trehalose may play a role in promoting water retention in cut flowers (Otsubo and Iwaya-Inoue, 2000).…”

Section: Effects Of Trehalose and Validamycin A On Quality Of Infloresupporting

confidence: 92%

Section: Roles Of Sucrose and Trehalosementioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

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Effects of Trehalose and Sucrose on the Vase Life and Physiology of Cut Astilbe (Astilbe × arendsii Arends) Flowers

et al. 2019

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“…5). TNBT staining reflects tissue viability by showing the quantity of active dehydrogenase in existence through observing purplish-black formazan as a result of reduced tetrazolium salts (Rosa and Tsou , 1961;Otsubo and Iwaya-Inoue, 2000). The petal tissues treated by TNBT were well stained in the orchid plants before ethylene exposure, particularly the parenchyma cells adjacent to the vascular bundles (Fig .…”

Section: Resultsmentioning

confidence: 99%

Petal Senescing Processes Monitored by Dynamic States of Water in Orchid Plants Exposed to Exogenous Ethylene

Iwaya‐Inoue

Watanabe

Tomizawa³

et al. 2005

ecb

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Ethylene induces premature senescence of orchid flowers when plants are particularly transported with fruits and vegetables stored nearby. Exogeneous ethylene regulated a complex physiological changes of petals in intact flowering clones of Dendrobium phalaenopsis during seven days; decrease in distance between petals, water content, NMR relaxation times (T1s and T2s) and degree in vital staining, while increase in ion leakage and translucence. Petal wilting and decrease in water content in florets were closely correlated with loss in viability of petal paren chyma tissues adjacent to the vascular bundles in the ethylene-exposed orchid flowers. Highly mobile water measured by T values of the long fraction in the petals was considered to free water derived from intact vacuoles. The loss of membrane integrity and free water of the orchid petals can be modulated by ethylene. After ethylene exposure treatment, the free water gradually decreased at 5 d. Additionally, T2s of the short fraction in the petals markedly decreased at 5 d after the ethylene exposure and they reached to 10 ms at 7 d. From these results, the decrease in the T1 and T2 of the orchid petals due to ethylene exposure indicated decreased vacuolar water, fol lowed by turgor loss and increased bound water, thus decrease in diffusion of substrates and me tabolism.

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“…Anserwadekar and Patil (1986) observed that 6 % sucrose was a good solution for prolonging the vase life of Gladiolus spikes, whereas Mayak et al (1973) and Bravdo et al (1974) found that higher sugar concentrations (up to 50 %) improve the opening and size of the florets and increase flower spike longevity, the amount of solution absorbed decreasing with increasing solution concentration. Treatment with 0.1 M trehalose, a disaccharide consisting of 2 linked glucose units, has been found to prolong vase life and improve spike quality whereas inhibitors and other sugars had no effect (Otsubo & Iwaya-Inoue, 2000;Yamane et al, 2005).…”

Section: Handling Of the Spikesmentioning

confidence: 99%