Trace Elements Analysis of Urine and Hair in Tuberculous Pleurisy

Bu, Xinhua; Wang, Guangqiang

doi:10.1007/s12011-011-8975-3

Cited by 3 publications

(1 citation statement)

References 19 publications

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“…After mycobacterium tuberculosis infection, macrophages in lesion site will be activated and IFN-γ content in pleural effusion and blood will gradually increase ( 20 ). Studies have shown that IL-27 has a dual regulatory effect on Th1-type immune responses in tuberculous diseases ( 21 , 22 ). On one hand, IL-27 can induce the differentiation of CD4 + in to Th1.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic value of T-Spot TB combined with INF-γ and IL-27 in tuberculous pleurisy

Zhang

Xiong

et al. 2017

Exp Ther Med

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The purpose of the present study was to investigate the diagnostic value of T cells spot test (T-Spot TB) combined with interferon-γ (INF-γ) and interleukin-27 (IL-27) in tuberculous pleurisy. Sixty patients with tuberculous pleurisy (observation group) and 60 patients with non-tuberculous pleurisy (control group) were enrolled in this study. T-Spot TB was performed to detect the pleural effusion of two groups of patients. Levels of IFN-γ and IL-27 in serum and pleural effusion were detected by enzyme-linked immunosorbent assay (ELISA). Relative expression of IFN-γ mRNA and IL-27 mRNA in peripheral blood mononuclear cells were detected by RT-PCR. Positive rate of T-Spot TB in observation group was 96.7% (58 cases), which was significantly higher than that in control group (p<0.05). Concentration of INF-γ in pleural effusion of observation group was 468.6±24.8 ng/l, which was significantly higher than that in control group (131.3±18.7 ng/l, p<0.05). Concentration of IL-27 in pleural effusion of observation group was 423.4±37.2 ng/l, which was significantly higher than that in control group (116.2±15.5 ng/l, p<0.05). Concentrations of INF-γ and IL-27 in serum of observation group were 48.2±13.4 and 41.7±10.6 ng/l, respectively, which were significantly higher than those in control group (38.6±11.2 and 35.3±8.4 ng/l, p<0.05). Relative expression levels of INF-γ mRNA and IL-27 mRNA in observation group were significantly higher than those in control group (p<0.05). Therefore, combination of T-Spot TB with INF-γ and IL-27 has significant application value in the clinical diagnosis of tuberculous pleurisy, and should be popularized.

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Section: Discussionmentioning

confidence: 99%

Diagnostic value of T-Spot TB combined with INF-γ and IL-27 in tuberculous pleurisy

Zhang

Xiong

et al. 2017

Exp Ther Med

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Comparison of sodium, potassium, calcium, magnesium, zinc, copper and iron concentrations of elements in 24-h urine and spot urine in hypertensive patients with healthy renal function

Chang

Liu

et al. 2017

Journal of Trace Elements in Medicine and Biology

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Estresse oxidativo e micronutrientes na hanseníase

Oliveira

Júnior

et al. 2015

Rev. Nutr.

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Objetivo Avaliar o estresse oxidativo, perfil antioxidante e de micronutrientes em pacientes portadores de hanseníase multibacilar e paucibacilar antes do tratamento poliquimioterápico. Métodos Analisaram-se 52 amostras de soro de pacientes portadores de hanseníase - 38 multibacilares e 14 paucibacilares -, usuários do ambulatório de dermatologia de um hospital público universitário, além de 30 amostras controles. Quantificaram-se marcador de peroxidação lipídica malondialdeído pelo método de substâncias reativas ao ácido tiobarbitúrico, antioxidante glutationa reduzida pelo método baseado na quantificação de tiol solúvel em ácido, antioxidante vitamina E por cromatografia líquida de alta eficiência, minerais selênio, zinco, cobre, magnésio por espectrometria de massa com fonte plasma acoplado, e sorologia do anticorpo glicolipídio fenólico I pelo método Enzyme-Linked Immunosorbent Assay. Foi utilizado teste não paramétrico de Mann-Whitney para comparar as variáveis quantificadas neste estudo entre os diferentes grupos, e correlação de Pearson para verificar associação dessas variáveis com o anticorpo. O critério de significância adotado foi de p<0,05. Resultados Houve diferença significativa para o malondialdeído (p<0,001) e vitamina E (p<0,001) no grupo controle comparado aos grupos com hanseníase, multibacilar e paucibacilar. No entanto, essas mesmas variáveis não diferiram entre os grupos multibacilar e paucibacilar (p=0,495 e p=0,920 respectivamente). A glutationa reduzida foi superior no grupo controle em relação ao grupo com hanseníase (p=0,012) e multibacilar (p=0,001), no entanto não diferiu do grupo paucibacilar (p=0,920). Quando comparada com os multibacilares e paucibacilares, a glutationa reduzida também não diferiu (p=0,063). Quanto aos minerais, todos se apresentaram dentro da normalidade, exceto o magnésio, cujos níveis foram deficientes em todos os pacientes do estudo. Não foi possível observar correlação do anticorpo glicolipídio fenólico I com as demais variáveis. Conclusão Os pacientes paucibacilares parecem possuir maior defesa antioxidante de glutationa reduzida, semelhante à de indivíduos saudáveis. Baixos níveis de vitamina E nos pacientes com hanseníase são sugestivos dos benefícios de suplementação. As alterações metabólicas observadas não evidenciaram relação com a sorologia do glicolipídio fenólico I.

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Trace Elements Analysis of Urine and Hair in Tuberculous Pleurisy

Cited by 3 publications

References 19 publications

Diagnostic value of T-Spot TB combined with INF-γ and IL-27 in tuberculous pleurisy

Diagnostic value of T-Spot TB combined with INF-γ and IL-27 in tuberculous pleurisy

Comparison of sodium, potassium, calcium, magnesium, zinc, copper and iron concentrations of elements in 24-h urine and spot urine in hypertensive patients with healthy renal function

Estresse oxidativo e micronutrientes na hanseníase

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