“…Abamectin was suspected causing adverse effects in the aquatic environment, even in low concentrations, as it presents high toxicity to aquatic organisms, such as fish and microcrustaceans (Tisler, Erzen, 2006). Several different methods have been reported in scientific literature for the analyses of abamectin, such as, high performance liquid chromatography (HPLC) (Kulik et al, 2011), liquid chromatography coupled to mass spectrometry (LC-MS) (Valenzuela et al, 2000;Pozo et al, 2003;Yoshii et al, 2004;Xiaolin et al, 2006;Thompson et al, 2009;Rübensam et al, 2011;Tao et al, 2012;Campillo et al, 2013;Ismail et al, 2013), HPLC with fluorescence (Diserens, Henzelin,1999;Souza et al, 2003;Kolar et al, 2004;Borges et al, 2008;Cerkvenik-Flajsa et al, 2010;Xie et al, 2011;Rahman et al, 2013), and UHPLC with fluorescence (Liu et al, 2001;Romero-González et al, 2011). The aim of this research was to validate a HPLC method for quantitative determination of abamectin homologs (H 2 B 1a and H 2 B 1b ) in gel formulation, as this drug is widely used in veterinary practice.…”