Toxicological effects of graphene oxide on adult zebrafish (Danio rerio)

Souza, José Paulo de; Baretta, Jéssica F.; Santos, Flávio Augusto Portela; Paino, Iêda Maria Martinez; Zucolotto, Valtencir

doi:10.1016/j.aquatox.2017.02.017

Cited by 98 publications

(55 citation statements)

References 32 publications

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“…Six months old adult zebrafish (both male and female) were exposed to sub-lethal concentrations (2, 10, and 20 mg/L) of GO (thickness 0.1 nm, average area 0.58 μm 2 ) either for 24 or 72 h as short-term exposure (for apoptosis, necrosis, DNA damage and oxidative stress) or for 14 days for chronic assay (histopathology) (Souza et al 2017) in fasting conditions. In short-term exposure, as a result of significant induction of ROS, the number of apoptotic and necrotic cells in gills were increased.…”

Section: Toxic Effects Of Gpnmentioning

confidence: 99%

“…Due to excellent morphological and biological features, Lin et al (2013) proposed to use zebrafish ( Danio rerio ) as an in vivo model for the study of environmental health and safety (EHS) aspects of engineered nanomaterials and nano-related products. Since then several excellent studies based on accumulation, morphology, behavior, neurological disorders, molecular mechanisms, and other potential targets of GPN have been conducted on embryos, larvae, adults, and transgenic zebrafish (Liu et al 2014; Chen et al 2015a,b; Jiang et al 2015; Mu et al 2015; Wang et al 2015; Ren et al 2016; d’Amora et al 2017; Li et al 2017; Lu et al 2017; Soares et al 2017; Souza et al 2017; Zhang et al 2017a; Zhang et al, 2017b). Studies have also been carried out with other fish species (Japanese medaka; Oryzias latipes ) (Mullick Chowdhury et al 2014; Li et al 2014).…”

Section: Introductionmentioning

confidence: 99%

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Graphene-Based Nanomaterials Toxicity in Fish

Dasmahapatra

Dasari

Tchounwou

2018

Reviews of Environmental Contamination and Toxicology

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Due to their unique physicochemical properties, graphene-based nanoparticles (GPN) constitute one of the most promising types of nanomaterials used in biomedical research. GPN have been used as polymeric conduits for nerve regeneration, carriers for targeted drug delivery, and in the treatment of cancer via photo-thermal therapy. Moreover, they have been used as tracers to study the distribution of bioactive compounds used in healthcare. Due to their extensive use, GPN released into the environment would probably pose a threat to living organisms and ultimately to human health. Their accumulation in the aquatic environment creates problems to aquatic habitats as well as to food chains. Until now the potential toxic effects of GPN are not properly understood. Despite agglomeration and long persistence in the environment, GPN are able to cross the cellular barriers successfully, entered into the cells and able to interact with all most all the cellular sites including the plasma membrane, cytoplasmic organelles, and nucleus. Their interaction with DNA creates more potential threats to both the genome and epigenome. In this brief review, we focused on fish, mainly zebrafish (Danio rerio), as a potential target animal of GPN toxicity in the aquatic ecosystem.

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Section: Toxic Effects Of Gpnmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Graphene-Based Nanomaterials Toxicity in Fish

Dasmahapatra

Dasari

Tchounwou

2018

Reviews of Environmental Contamination and Toxicology

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“…Fabrication of GO was carried out following the Hummers method using graphite provided by Nacional de Grafite (Brazil). Details on the GO synthesis could be found in Reference .…”

Section: Methodsmentioning

confidence: 99%

Fabrication of random and aligned electrospun nanofibers containing graphene oxide for skeletal muscle cells scaffold

Uehara

Paino

Santos

et al. 2020

Polymers for Advanced Techs

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Graphene oxide (GO)-based materials have been explored in biomedical applications as active engineered materials for diagnosis and therapy. Although a large number of studies have been carried out in the last years, aspects involving the orientation and elongation of cells on GO immobilized on polymeric nanofibers are still scarce. We investigated the interactions between skeletal muscle cells and GO immobilized on random and aligned electrospun nanofibers of poly(caprolactone) (PCL), a biocompat-

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“…Gills are responsible for gas exchange, carbon dioxide release, and the exchange of salt and water, and play a major role in the excretion of nitrogenous waste products, primarily ammonia (25). Structural damage induced by GO therefore makes the fish vulnerable to osmotic deregulation as well as respiratory difficulties (37). In another study, GO administration was found to induce dosedependent lung toxicity, granulomatous abrasions and injuries, and inflammatory cell penetration (30).It has been demonstrated that the increased mucus layer and cell hyperplasia may constitute a barrier for NP uptake by the gills (34).…”

Section: Graphene Expression Multiple Histological Changes (A) Seconmentioning

confidence: 99%

Histopathological Changes (Gills and Liver) and Clinical Signs of Common Carp, Cyprinus Carpio L. Exposed to Graphene Nanoparticles

Khalel¹

2019

IQ J Agr Scs

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This study was conducted to investigate the effect of graphene nanoparticles on histopathological changes and clinical signs of common carp Cyprinus carpio. A total of 48 fish were used in the experiment with an average weight 68.44 g. Fish were exposed to graphene nanoparticles at concentrations of 10 and 20 mg / L for the T1 and T2 respectively, as well as to control group (without graphene nanoparticles). Fish exposed to graphene showed no abnormal behavior and no mortality satisfactory clinical signs in the experimental fish. Histopathological changes of gills showed fusion with shorting of the secondary lamellae, lifting up secondary lamellae beside necrosis and hyperplasia of the epithelial cells of secondary lamellae in gills. On the other hand, there was necrosis of hepatocytes, dilation of sinusoids, hydrobic degeneration, hepatocellular vacuolation, hypertrophy and hydrobic of degeneration in liver. The present study was suggested to investigate of the graphene nanoparticles effects on histopathological changes and clinical signs to clarify a clear representation about on fish and its danger of its accumulation in aquatic environment.

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Toxicological effects of graphene oxide on adult zebrafish (Danio rerio)

Cited by 98 publications

References 32 publications

Graphene-Based Nanomaterials Toxicity in Fish

Graphene-Based Nanomaterials Toxicity in Fish

Fabrication of random and aligned electrospun nanofibers containing graphene oxide for skeletal muscle cells scaffold

Histopathological Changes (Gills and Liver) and Clinical Signs of Common Carp, Cyprinus Carpio L. Exposed to Graphene Nanoparticles

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