Fabrication of random and aligned electrospun nanofibers containing graphene oxide for skeletal muscle cells scaffold

Uehara, Thiers M.; Paino, Iêda Maria Martinez; Santos, Flávio Augusto Portela; Scagion, Vanessa P.; Corrêa, Daniel S.; Zucolotto, Valtencir

doi:10.1002/pat.4874

Cited by 24 publications

(24 citation statements)

References 38 publications

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“…Raman spectroscopy analyses confirmed the presence of GO/PCL nanofibers and DSPC monolayer/GO scaffolds, by investigating the functional GO groups as displayed in Figure 5. The bands at 1350 and 1600 cm −1 were observed, which are characteristics of the D and G bands from GO, respectively 32,40 . These resonances are more intense for PCL nanofibers scaffolds containing a larger amount of GO due to the change of physical–chemical surface after plasma treatment.…”

Section: Resultsmentioning

confidence: 92%

Nanostructured scaffolds containing graphene oxide for nanomedicine applications

Uehara

Migliorini

Facure

et al. 2021

Polymers for Advanced Techs

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The use of graphene oxide (GO) has become widespread due to its advantageous properties for applications in medical devices, including cell scaffolds and sensors.Investigations on the spectroscopic and electrochemical features of nanostructured cell scaffolds may be of interest to design novel scaffolds architectures aimed at understanding their interactions with healthy and cancer cells. In this study, we investigated the interactions between liver cancer cells and two GO-containing scaffold platforms, namely: cells membrane models containing GO in the form of Langmuir-Blodgett films, and GO-modified biodegradable polycaprolactone nanofibers. Sumfrequency generation spectroscopy revealed the presence and formation of an expanded phospholipid monolayer underneath GO, while scanning electron microscopy images revealed the morphology of the cells on the different surfaces. Electrochemical impedance spectroscopy was employed to evaluate the charge transfer resistance in different nanostructured scaffolds containing liver cancer cells. The nanosystems developed here can be applied to study the interactions between cells on polymer nanofibers and Langmuir-Blodgett films modified with GO for regenerative medicine.

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Section: Resultsmentioning

confidence: 92%

Nanostructured scaffolds containing graphene oxide for nanomedicine applications

Uehara

Migliorini

Facure

et al. 2021

Polymers for Advanced Techs

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“…[ 139–141 ] The surface modification can be tailored mainly through the concentration of the solution and the immersion time. [ 142 ] This method allows the functionalization of nano‐/microfibers with distinct nanostructures as Co 3 O 4, [ 143 ] AgNWs, [ 144 ] TiO 2 , [ 145,146 ] SiO 2 , [ 147 ] Al 2 O 3 , [ 148 ] ZnO, [ 132,149 ] AgPO 4, [ 150 ] AgNPs, [ 151,152 ] graphene oxide, [ 153 ] reduced graphene oxide (rGO), [ 154 ] CNW(cellulose nanowhiskers):Ag, [ 155 ] CNW:Au, [ 156 ] sugarcane bagasse fly ash (SBFA), [ 157 ] MWCNTs, [ 158 ] and AgNPs/rGO. [ 159 ]…”

Section: Post‐modification Methods For Micro/nanofibermentioning

confidence: 99%

“…The nanomaterial obtained improved the osteogenic differentiation of unrestricted somatic stem cells. Uehara and co‐workers [ 153 ] employed oxygen plasma treatment to modify the surface of poly(caprolactone) (PCL) nanofibers and improve graphene oxide anchoring, aiming to investigate the interaction of hybrid nanofibers with skeletal muscle cells designed for tissue engineering. In another work, the oxygen plasma was employed to increment PMMA nanofibers hydrophilicity by changing the duration of treatment (10 or 30 s), which facilitated the growth of silver nanoparticles on their surface.…”

Section: Post‐modification Methods For Micro/nanofibermentioning

confidence: 99%

Tailoring the Surface Properties of Micro/Nanofibers Using 0D, 1D, 2D, and 3D Nanostructures: A Review on Post‐Modification Methods

Schneider

Facure

Chagas

et al. 2021

Adv Materials Inter

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Micro‐ and nanofibers produced by electrospinning and solution blow spinning (SBS) are highly suitable platforms for diverse applications due to their advantageous properties, including the high surface area to volume ratio, high porosity, and flexibility. To render them additional functionalities, distinct pre‐ or post‐modification processes have been proposed for modifying such micro‐ and nanofibers with 0D, 1D, 2D, and 3D nanostructures. The pre‐modification requires the addition of 0D–3D nanostructures (or their precursors) into the polymeric phase before the spinning process, which can demand laborious solubilization and requires changes in experimental spinning parameters, with impact on morphology, spinnability, and properties. Post‐modification methods, on the other hand, enable the fabrication of composite fibers without the need to optimize the spinning parameters, allowing a simple and efficient surface modification. Herein, recent advances on post‐modification methods of spun fibers, including wet chemistry, grafting, crosslinking, click chemistry, oxidations, hydrolysis and reduction strategies, dip‐coating, layer‐by‐layer, electro/air‐spray, atomic layer deposition, and plasma techniques aiming at the surface functionalization with 0D–3D nanostructures are surveyed. Recent results, trends, and challenges on the application of such surface‐modified fibers for environmental, industrial, and medical applications, including as adsorbent and filtering membranes, catalysts for pollutants degradation, and wearable sensors are examined.

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“…Similar to the nerves, muscles (skeletal, cardiac, and smooth) have an electrical stimuli-responsive characteristic; thus, the utilization of conductive biomaterials was proposed. There was no difference in cell adhesion and cellular growth in a random and aligned GO electrospun mat, which was modified with oxygen plasma [ 43 ]. GO-polyurethane (PU) foam is considered to be a beneficial scaffold for myogenesis in skeletal tissue engineering.…”

Section: Development Of Tissues and Organs Using Graphene-based Materialsmentioning

confidence: 99%

Graphene Oxide: Opportunities and Challenges in Biomedicine

et al. 2021

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Desirable carbon allotropes such as graphene oxide (GO) have entered the field with several biomedical applications, owing to their exceptional physicochemical and biological features, including extreme strength, found to be 200 times stronger than steel; remarkable light weight; large surface-to-volume ratio; chemical stability; unparalleled thermal and electrical conductivity; and enhanced cell adhesion, proliferation, and differentiation properties. The presence of functional groups on graphene oxide (GO) enhances further interactions with other molecules. Therefore, recent studies have focused on GO-based materials (GOBMs) rather than graphene. The aim of this research was to highlight the physicochemical and biological properties of GOBMs, especially their significance to biomedical applications. The latest studies of GOBMs in biomedical applications are critically reviewed, and in vitro and preclinical studies are assessed. Furthermore, the challenges likely to be faced and prospective future potential are addressed. GOBMs, a high potential emerging material, will dominate the materials of choice in the repair and development of human organs and medical devices. There is already great interest among academics as well as in pharmaceutical and biomedical industries.

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Fabrication of random and aligned electrospun nanofibers containing graphene oxide for skeletal muscle cells scaffold

Cited by 24 publications

References 38 publications

Nanostructured scaffolds containing graphene oxide for nanomedicine applications

Nanostructured scaffolds containing graphene oxide for nanomedicine applications

Tailoring the Surface Properties of Micro/Nanofibers Using 0D, 1D, 2D, and 3D Nanostructures: A Review on Post‐Modification Methods

Graphene Oxide: Opportunities and Challenges in Biomedicine

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