Toxicological Assessment of Ammonia Exposure on Carassius auratus red var. Living in Landscape Waters

Hao, Ming; Zuo, Qiting; Zhang, Wei; Feng, Yakun; Wang, Li; Yu, Luji; Zhang, Xu; Li, Jing; Huang, Zehan

doi:10.1007/s00128-019-02728-5

Cited by 7 publications

(1 citation statement)

References 35 publications

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“…Our current study found that ammonia decreased the GSH content in fish macrophages. Similar results were also detected in the hepatopancreas of Carassius auratus red var (Hao et al, 2019) and juvenile turbot (Scophthalmus maximus) (Jia et al, 2017). Thus, we speculated that ammonia stress might induce GSH depletion to interfere the normal function of GPX4, resulting in the elevation of ROS level in fish macrophages.…”

Section: Ta B L E 1 Real-time Pcr Primer Sequencessupporting

confidence: 77%

Ammonia triggers ferroptosis in macrophages of yellow catfish Pelteobagrus fulvidraco

Gan

Yao

Zhang

et al. 2021

Aquaculture Research

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An in vitro experiment was carried out to investigate the effect of ammonia on the macrophages of yellow catfish Pelteobagrus fulvidraco from a ferroptotic point of view.The fish macrophages were cultured with 0.00, 0.12, 0.23 and 0.46 mg/L ammonia for 24 h. The result showed that ammonia decreased the cell viability, reduced glutathione (GSH) contents and increased the reactive oxygen species (ROS) levels of fish macrophages. Meanwhile, the mRNA levels of systemX − c and ferritin light chain1 (FTL1) were down-regulated when ammonia concentration was elevated up to 0.12 mg/L.Compared with control, the mRNA level of glutathione peroxidase 4 (GPX4) was upregulated when ammonia was elevated up to 0.12 mg/L, but was down-regulated when ammonia reached 0.46 mg/L. Compared with control, the mRNA level of lipoxygenase (LOX)-12 was up-regulated when ammonia was elevated up to 0.12 mg/L, but was down-regulated when ammonia reached 0.23 mg/L, and the mRNA levels of LOX-5, LOX-15b and transferrin Receptor 1 (TFR1) were up-regulated when ammonia was elevated up to 0.12 mg/L, but was down-regulated when ammonia reached 0.23 mg/L. In conclusion, our study found that ammonia could trigger ferroptosis in the macrophage of fish.

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Section: Ta B L E 1 Real-time Pcr Primer Sequencessupporting

confidence: 77%