Toxicity of pesticides toward human immune cells U-937 and HL-60

Barbasz, Anna; Kreczmer, Barbara; Skórka, Magdalena; Czyżowska, Agnieszka

doi:10.1080/03601234.2020.1777059

Cited by 15 publications

(11 citation statements)

References 34 publications

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“…Overall, these data document the safety of low WD doses, with no induction of inflammatory features. The induction of inflammation is a common effect of many chemicals used in the agri-food industry, and chronic exposure could affect the well-being of workers [21][22][23]. Therefore, the absence of inflammatory reactions found in our study, strengthens the safe profile of diluted WD.…”

Section: The Exposure Of Cells To Wd Did Not Induce An Inflammatory P...supporting

confidence: 72%

“…To this purpose, we assessed the effect of increasing concentrations of sweet chestnut WD on human cell models, representative of epidermis, mucosal membranes, and other cells on which WD could interact following exposure or absorption. Indeed, most of the chemical products manipulated in the agri-food industry are assimilated transcutaneously or by inhalation, causing inflammation and hazard to health [21][22][23]. We thus investigated the behavior, after exposure (short and prolonged) to WD, of an immortalized keratinocyte cell model (HaCaT) that represents the major cell type of the epidermis [24], of an epidermoid carcinoma (A431), extensively used for studies on the effect of xenobiotics in human skin and mucosa [25][26][27] and of normal human dermal fibroblasts (NHDF), stromal cells involved in the control of skin homeostasis and a balanced wound healing process [28].…”

Section: Introductionmentioning

confidence: 99%

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Characterization of the Safety Profile of Sweet Chestnut Wood Distillate Employed in Agriculture

Filippelli

Ciccone

Morbidelli

2021

Safety

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In organic agriculture, synthetic pesticides and treatments are substituted by natural remedies with interesting success for product yield and environmental outcomes, but the safety of these bio-based products needs to be assessed in vertebrate and human models. Therefore, in this paper we assessed the safety profile of sweet chestnut (Castanea sativa) wood distillate (WD) on the different cellular components of tissues implied in transcutaneous absorption. We investigated the viability of different cell lines mimicking the skin (HaCaT keratinocytes), mucosa (A431), connective (normal human dermal fibroblasts, NHDF) and vascular (human umbilical vein endothelial cells, HUVEC) tissues after exposure to increasing concentrations (0.04–0.5%, v/v, corresponding to 1:2800–1:200 dilutions) of WD. A short exposure to increasing doses of WD was well tolerated up to the highest concentration. Instead, following a prolonged treatment, a concentration dependent cytotoxic effect was observed. Notably, a different behavior was found with the various cell lines, with higher sensitivity to cytotoxicity by the cells with higher proliferation rate and reduced doubling time (human keratinocytes). Moreover, to exclude an inflammatory effect at the not cytotoxic WD concentrations, the expression of the main inducible markers of inflammation, cyclooxygenase-2 (COX-2) and microsomal prostaglandin E synthase-1 (mPGES-1), were assessed, and no improvement was found both after brief and prolonged exposure. In conclusion, our data exclude any inflammatory and cytotoxic effect at the lowest WD concentrations, namely 0.07% and 0.04%, mimicking some recommended dilutions of the product and the potential exposure doses for the operators in agriculture. Nevertheless, higher concentrations showed a safe profile for short time usage, but caution should be used by farmers following persistent product exposure.

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Section: The Exposure Of Cells To Wd Did Not Induce An Inflammatory P...supporting

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Characterization of the Safety Profile of Sweet Chestnut Wood Distillate Employed in Agriculture

Filippelli

Ciccone

Morbidelli

2021

Safety

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“…The altered number of leukocytes was also associated to an increase in the production of the inflammatory cytokine IL-6 in exposed farmers [52]. In another in-vitro study, performed by Barbasz et al [53], the potential toxic effect of three pesticides, including glyphosate, was tested on two human immune-cell lines: the human histiocytic lymphoma cell line (U-937) and the human promyelocytic cell line (HL-60). The concentration of pesticide chosen to treat cell lines was equivalent to the amount to which farmers or the general population are usually exposed.…”

Section: Glyphosate-induced Effects In Blood Cellsmentioning

confidence: 99%

“…From a cell-vitality analysis, it was shown that cells, treated for 24 h with 3600 µg/mL of glyphosate, survived only in 20% of cases as compared with controls, thus corroborating previous results on the cytotoxicity of this pesticide. Lipid peroxidation was also assessed by determining the MDA concentration: the MDA concentration in the U-937 cell line increases four times more than the control group, suggesting that glyphosate cytotoxicity could be mediated by the induction of strong oxidative stress [53].…”

Section: Glyphosate-induced Effects In Blood Cellsmentioning

confidence: 99%

Pleiotropic Outcomes of Glyphosate Exposure: From Organ Damage to Effects on Inflammation, Cancer, Reproduction and Development

Marino

Mele

Viggiano

et al. 2021

IJMS

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Glyphosate is widely used worldwide as a potent herbicide. Due to its ubiquitous use, it is detectable in air, water and foodstuffs and can accumulate in human biological fluids and tissues representing a severe human health risk. In plants, glyphosate acts as an inhibitor of the shikimate pathway, which is absent in vertebrates. Due to this, international scientific authorities have long-considered glyphosate as a compound that has no or weak toxicity in humans. However, increasing evidence has highlighted the toxicity of glyphosate and its formulations in animals and human cells and tissues. Thus, despite the extension of the authorization of the use of glyphosate in Europe until 2022, several countries have begun to take precautionary measures to reduce its diffusion. Glyphosate has been detected in urine, blood and maternal milk and has been found to induce the generation of reactive oxygen species (ROS) and several cytotoxic and genotoxic effects in vitro and in animal models directly or indirectly through its metabolite, aminomethylphosphonic acid (AMPA). This review aims to summarize the more relevant findings on the biological effects and underlying molecular mechanisms of glyphosate, with a particular focus on glyphosate's potential to induce inflammation, DNA damage and alterations in gene expression profiles as well as adverse effects on reproduction and development.

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“…Moreover, the scope of this industrial pollution’s impact is quoted to be vast in nature and is one of the leading causes of deforestation (Murshed et al 2020 ), natural resource depletion (Hussain et al 2020 ), elimination of ecology (Sejati et al 2020 ), rising global temperatures (Mahmood et al 2020 ), melting glaciers (Kumar et al 2020 ), rising sea levels, floods (Yoo and Suh 2020 ), frequent natural disasters (Chandio et al 2020 ), crops destruction and rising food insecurities (Gupta et al 2020 ), and rising health issues due to Covid-19 as well (Coccia 2020 ). This is mainly because of the weak immunity levels of people, as a result of exposure to industrial pollution over a long period of time (Barbasz et al 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Mapping green technologies literature published between 1995 and 2019: a scientometric review from the perspective of the manufacturing industry

Usmani

Wang

Ahmad

et al. 2021

Environ Sci Pollut Res

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This study presents a comprehensive literature review and gives an insight into the increasing research trends that are based on the discipline of green technology (GTs) in the manufacturing industry. Prior research in this field indicates that there is a scarcity of research on the topic. Therefore, this study seeks to draft a multi-perspective literature review that is based on GTs in the manufacturing industry. Moreover, to make this analysis more detailed, the science-mapping technique and the quantitative approach were also applied on 5734 bibliographic references that were extracted from the web of science. Ultimately, the focus of the research is to understand the tendencies and trends in journals, institutions, and the main areas of research, along with the integration style of these elements in the previous literature that has been written on the subject of GTs. This technique also helps to fill in the research gap, address the limitations of existing literature, and shed light on the various possible directions this could lead to for future research. The implications of this research offer wide directives for editors, researchers, research institutions, policymakers, and practitioners.

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Toxicity of pesticides toward human immune cells U-937 and HL-60

Cited by 15 publications

References 34 publications

Characterization of the Safety Profile of Sweet Chestnut Wood Distillate Employed in Agriculture

Characterization of the Safety Profile of Sweet Chestnut Wood Distillate Employed in Agriculture

Pleiotropic Outcomes of Glyphosate Exposure: From Organ Damage to Effects on Inflammation, Cancer, Reproduction and Development

Mapping green technologies literature published between 1995 and 2019: a scientometric review from the perspective of the manufacturing industry

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