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DNA oxidative damage is one of the main concerns being implicated in severe cell alterations, promoting different types of human disorders and diseases. For their characteristics, male gametes are the most sensitive cells to the accumulation of damaged DNA. We have recently reported the relevance of arginine residues in the Cu(II)-induced DNA breakage of sperm H1 histones. In this work, we have extended our previous findings investigating the involvement of human sperm nuclear basic proteins on DNA oxidative damage in healthy males presenting copper and chromium excess in their semen. We found in 84% of those males an altered protamines/histones ratio and a different DNA binding mode even for those presenting a canonical protamines/histones ratio. Furthermore, all the sperm nuclear basic proteins from these samples that resulted were involved in DNA oxidative damage, supporting the idea that these proteins could promote the Fenton reaction in DNA proximity by increasing the availability of these metals near the binding surface of DNA. In conclusion, our study reveals a new and unexpected behavior of human sperm nuclear basic proteins in oxidative DNA damage, providing new insights for understanding the mechanisms related to processes in which oxidative DNA damage is implicated.

The originality of form is one of the new trends that can be identified in the current design of tall buildings. In this design trend, the so-called diagrid structures, which represent the latest mutation of tubular structures, play a major role due to their inherent esthetic quality, structural efficiency and geometrical versatility. In this paper, an overview on application of such typology to high-rise buildings is carried out; in particular, in the first part of the paper, the peculiarities of diagrid systems are described: starting from the analysis of the internal forces arising in the single diagrid module due to vertical and horizontal loads, the resisting mechanism of diagrid buildings under gravity and wind loads is described, and recent researches and studies dealing with the effect of geometry on the structural behavior are discussed. In the second part of the paper, a comparative analysis of the structural performance of some recent diagrid tall buildings, characterized by different number of stories and different geometries, namely the Swiss Re building in London, the Hearst Headquarters in New York and the West Tower in Guangzhou, is carried out, and some general design remarks are derived

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The procedures and formulations suggested in literature for the design of diagrid structures start from the assumption that diagonal sizing process is governed by the stiffness requirements, as usually occurs for other, less efficient, structural types, and that member strength demand is automatically satisfied by the cross section resulting from the stiffness requirements. However, thanks to the high rigidity of the diagonalized façade, strength requirements can be of paramount importance and even be the governing design criterion. In this paper, stiffness and strength design criteria for diagrid structures are examined and translated in simplified formulae for quick member sizing. The application of the two approaches for the design of a 100-storey building model, carried out for different diagrid geometrical patterns, gives the opportunity of discussing the relative influence of stiffness and strength on the design outcomes, in terms of resulting diagonal cross sections and steel weight, as well as on the structural performance