Toxicity of a synthetic phenolic antioxidant, butyl hydroxytoluene (BHT), in vertebrate model zebrafish embryo (
            <i>Danio rerio</i>
            )

Sarmah, Raktim; Bhagabati, Sarada Kanta; Dutta, Rajdeep; Nath, Dipanka; Pokhrel, Hemanta; Mudoi, Lawonu Prasad; Sarmah, Nishi; Sarma, Jiten; Ahmed, Abdul Malik; Nath, Rupam Jyoti; Ingtipi, Lucy; Kuotsu, Kedolhouse

doi:10.1111/are.14732

Cited by 33 publications

(11 citation statements)

References 38 publications

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“…Previous research have shown the toxicity of BHT in the development of zebrafish embryos/larvae [ 33 , 43 ]. Other studies have shown that BHT and these transformation products present an endocrine-disrupting effect, which promotes the development of tumors and cancer in humans [ 9 , 32 , 33 ] and may also have a potential teratogenic effect on aquatic organisms [ 44 ].…”

Section: Antioxidantsmentioning

confidence: 99%

Detection of Synthetic Antioxidants: What Factors Affect the Efficiency in the Chromatographic Analysis and in the Electrochemical Analysis?

Gonçalves-Filho

Souza

2022

Molecules

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Antioxidants are food additives largely employed to inhibit oxidative reactions in foodstuffs rich in oils and fat lipids, extending the shelf life of foodstuffs and inhibiting alterations in color, flavor, smell, and loss of nutritional value. However, various research has demonstrated that the inadequate use of synthetic antioxidants results in environmental and health problems due to the fact that some of these compounds present toxicity, and their presence in the human body, in high concentrations, is related to the development of some cancer types and other diseases. Therefore, the development of analytical methods for identifying and quantifying synthetic antioxidants in foodstuffs is fundamental to quality control and in ensuring consumer food safety. This review describes the recent chromatographic and electrochemical techniques used in the detection of synthetic phenolic antioxidants in foodstuffs, highlighting the main characteristics, advantages and disadvantages of these methods, and specific typical features, which include extraction methods for sample preparation and materials used in the working electrode construction, considering chromatographic and voltammetric methods, since these specific features influence the efficiency in the analysis.

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Section: Antioxidantsmentioning

confidence: 99%

Detection of Synthetic Antioxidants: What Factors Affect the Efficiency in the Chromatographic Analysis and in the Electrochemical Analysis?

Gonçalves-Filho

Souza

2022

Molecules

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“…Previous studies in developmental zebrafish have demonstrated conflicting results regarding BHT-induced toxicity. Multiple studies suggest BHT is toxic, with a 96-h LC50 ranging from ~20–60 µM and larval behavior being affected at as low as 1 µM [ 57 , 61 ]; however, a separate study did not find significant lethality up to 200 µM, though physical malformations were observed below 100 µM [ 58 ]. The inconsistency between our findings and those of previous studies may be attributed to variable exposure conditions, including chorion status and exposure in pools versus individual wells, both of which can affect the actual internal dose of a chemical [ 62 , 63 , 64 ].…”

Section: Discussionmentioning

confidence: 99%

A Comparative Multi-System Approach to Characterizing Bioactivity of Commonly Occurring Chemicals

Rivera

Wilson

Kim

et al. 2022

IJERPH

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A 2019 retrospective study analyzed wristband personal samplers from fourteen different communities across three different continents for over 1530 organic chemicals. Investigators identified fourteen chemicals (G14) detected in over 50% of personal samplers. The G14 represent a group of chemicals that individuals are commonly exposed to, and are mainly associated with consumer products including plasticizers, fragrances, flame retardants, and pesticides. The high frequency of exposure to these chemicals raises questions of their potential adverse human health effects. Additionally, the possibility of exposure to mixtures of these chemicals is likely due to their co-occurrence; thus, the potential for mixtures to induce differential bioactivity warrants further investigation. This study describes a novel approach to broadly evaluate the hazards of personal chemical exposures by coupling data from personal sampling devices with high-throughput bioactivity screenings using in vitro and non-mammalian in vivo models. To account for species and sensitivity differences, screening was conducted using primary normal human bronchial epithelial (NHBE) cells and early life-stage zebrafish. Mixtures of the G14 and most potent G14 chemicals were created to assess potential mixture effects. Chemical bioactivity was dependent on the model system, with five and eleven chemicals deemed bioactive in NHBE and zebrafish, respectively, supporting the use of a multi-system approach for bioactivity testing and highlighting sensitivity differences between the models. In both NHBE and zebrafish, mixture effects were observed when screening mixtures of the most potent chemicals. Observations of BMC-based mixtures in NHBE (NHBE BMC Mix) and zebrafish (ZF BMC Mix) suggested antagonistic effects. In this study, consumer product-related chemicals were prioritized for bioactivity screening using personal exposure data. High-throughput high-content screening was utilized to assess the chemical bioactivity and mixture effects of the most potent chemicals.

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“…The toxic effects of food additives on zebrafish were mainly found to be in the form of dose‐dependent developmental toxicity (Sarmah et al., 2020), cardiotoxicity (Huang et al., 2021), and reproductive toxicity (Bereketoglu & Pradhan, 2019). Azo dyes are widely used as colorants in the food industry, especially in candy; thus, these dyes may have more effects on children than adults.…”

Section: Research Progress In the Toxicological Assessment Of Hazards...mentioning

confidence: 99%

Zebrafish as model organisms for toxicological evaluations in the field of food science

Hou

Liu

Qin

et al. 2023

Comp Rev Food Sci Food Safe

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Food safety has long been an area of concern. The selection of stable and efficient model organisms is particularly important for food toxicology studies. Zebrafish (Danio rerio) are small model vertebrates, and 70% of human genes have at least one zebrafish ortholog. Zebrafish have advantages as model organisms due to their short life cycle, strong reproductive ability, easy rearing, and low cost. Zebrafish embryos have the advantage of being sensitive to the breeding environment and thus have been used as biosensors. Zebrafish and their embryos have been widely used for food toxicology assessments. This review provides a systematic and comprehensive summary of food toxicology studies using zebrafish as model organisms. First, we briefly introduce the multidimensional mechanisms and structure–activity relationship studies of food toxicological assessment. Second, we categorize these studies according to eight types of hazards in foods, including mycotoxins, pesticides, antibiotics, heavy metals, endocrine disruptors, food additives, nanoparticles, and other food‐related ingredients. Finally, we list the applications of zebrafish in food toxicology studies in line with future research prospects, aiming to provide a valuable reference for researchers in the field of food science.

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Toxicity of a synthetic phenolic antioxidant, butyl hydroxytoluene (BHT), in vertebrate model zebrafish embryo ( Danio rerio )

Cited by 33 publications

References 38 publications

Detection of Synthetic Antioxidants: What Factors Affect the Efficiency in the Chromatographic Analysis and in the Electrochemical Analysis?

Detection of Synthetic Antioxidants: What Factors Affect the Efficiency in the Chromatographic Analysis and in the Electrochemical Analysis?

A Comparative Multi-System Approach to Characterizing Bioactivity of Commonly Occurring Chemicals

Zebrafish as model organisms for toxicological evaluations in the field of food science

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