Toxicity Evaluation of Yoyo 'Cleanser' Bitters and Fields Swedish Bitters Herbal Preparations following Sub-Chronic Administration in Rats

Abstract

Problem statement:Owing to numerous adverse effects of most orthodox pharmaceuticals, the use of herbal remedies in treatment and prevention of diseases is gaining much attention, especially in developing countries. The increase patronage of these remedies is often predicated on the assumption that they are harmless since they are prepared from natural sources. However, toxicity related to herbal medicines is becoming widely recognized. Yoyo 'Cleanser' Bitters (YB) and Fields Swedish Bitters (SB) are herbal preparations largely consumed in Nigeria because of their high publicity in the various national news media. This study evaluated their toxicity potentials following sub-chronic administration in rats. Approach: Thirty-five Wistar rats were divided into five groups of 7 rats/group. Control received normal saline while the experimental groups were treated separately with 15 and 30 mL kg −1 of YB and SB respectively. Treatment was given daily by gavage for 30 days. Body weights were measured weekly and rats were sacrificed 24 h after last treatment. Blood was collected by cardiac puncture for biochemical and haematological assessment. Vital organs were also excised and weighed. Results: YB and SB did not significantly affect creatinine, blood urea nitrogen, Na + ion but significantly (p<0.01) decreased K + ion concentration when compared with control. Both herbal preparations produced moderate increases in alanine and aspartate aminotransferase activities, with YB (30 mL kg −1 ) significantly (p<0.05) increasing the latter. YB and SB did not significantly alter total cholesterol, triglycerides and albumin but SB (30 mL kg −1 ) significantly (p<0.05) decreased total protein when compared with control. SB significantly (p<0.05) elevated white blood cell count while other haematological parameters, vital organs and body weights were not significantly affected by both herbal preparations. Conclusion: YB and SB possess the risk of inducing hypokalaemia. The potential to also increase markers of liver function calls for cautious use in conditions associated with liver impairment.

Yoyo Bitters, a polyherbal formulation influenced some biochemical parameters in Wistar rats

“…However, no correlations were found between ALP and PO 4 3 -levels in rat serum, kidney and liver (r-value = 0.025, 0.008, 0.045, respectively). Our observation is in agreement with a previous report by Ekor et al (5) , which demonstrated that administration of Yoyo Bitters to rats for 30 days did not signifi cantly affect rat plasma and tissue levels of protein, ALP, creatinine, K + and Na + .…”

The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety

“…Numerous and irrefutable cases of poisoning have been reported in the literature ( Vanherweghem and Degaute, 1998 ; Cosyns et al, 1999 ; Ernst, 2002 ). The toxicity evaluation of the polyherbal formula, Yoyo “Cleanser” Bitters®, conducted recently in our laboratory ( Ekor et al, 2010 ), was prompted by an unpublished case report of a young male adult who had been on self-medication with this herbal product and was subsequently admitted to the hospital on account of liver failure. Yoyo “Cleanser” Bitters® is one of the herbal remedies that is widely advertised in the various Nigerian media and as such has gained so much public acceptance over time and continues to enjoy increased patronage among consumers, especially in the southwestern part of the country.…”

Lipid Profile and Oxidative Stress Markers in Wistar Rats following Oral and Repeated Exposure to Fijk Herbal Mixture

“…The popular belief that herbal remedies are without toxic or undesirable effects has however often been proven otherwise [1, 5, 9]. …”

“…Furthermore, the general belief that herbal medicines are natural, effective, and without adverse effects has immensely contributed to the upsurge in the patronage of herbal formulations [ 6 – 8 ]. The popular belief that herbal remedies are without toxic or undesirable effects has however often been proven otherwise [ 1 , 5 , 9 ].…”