Toxic effects of dexamethasone on mouse testicular germ cells

Orazizadeh, Mahmoud; Khorsandi, Layasadat; Hashemitabar, Mahmoud

doi:10.1111/j.1439-0272.2009.00985.x

Cited by 59 publications

(48 citation statements)

References 39 publications

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“…For this purpose we measured the external diameter and the lumen diameter of 150 seminiferous tubules per animal by fitting a graticule of a calibrated linear scale in the ×10 eyepiece of Leitz microscope at objective lens ×40 using of calibrated Motic software. For measuring the tubular diameter was subtracted from luminar diameter (Orazizadeh et al, 2010). TUNEL Assay.…”

Section: Animals-inberdmentioning

confidence: 99%

Protective Effect of Tribulus terrestris Hydroalcoholic Extract Against CisplatinInduced Apoptosis on Testis in Mice

et al. 2015

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SUMMARY:Cisplatin is an anti-cancer drug used in chemotherapy. One of the limiting side effects of cisplatin is decreasing genital gland function, azoospermia and oligospermia. Tribulus terrestris (TT) has been used as an aphrodisiac. The present study amid to investigate protective effect of TT hydroalcoholic extract against cisplatin-induced apoptosis on testis in mice. Male adult mice (n=30) were divided into control and 4 experimental groups (n=6). Control group received saline, first experimental group received cisplatin (5.5 mg/kg) and other three experimental group received cisplatin (5.5 mg/kg) and different doses of hydroalcoholic extact of TT (100, 300 and 500 mg/kg/i.p) respectively. Day after the last injection, histopathology and histomorphic analysis and also TUNEL assay on mice testis were performed. Weights of body and testis, seminiferous tubules diameter and apoptotic index were assessed. Data analysis was performed using one-way ANOVA followed by Tukeys' test. The results showed that cisplatin lead to a reduction in the weight of body and testes, and significantly increased apoptotic index compared to the control group (P<0.001), while in treated groups with TT, the weights of body and testis and seminiferous tubules diameter were significantly higher compared with cisplatin group (P<0.001), but apoptotic index did not show significant differences. The study demonstrates that extract of TT could protective effect of on cisplatin-induced apoptosis of testis and seminiferous tubules diameter that may be related to the presence of antioxidant components acting via a multitude of central and peripheral mechanisms

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Section: Animals-inberdmentioning

confidence: 99%

Protective Effect of Tribulus terrestris Hydroalcoholic Extract Against CisplatinInduced Apoptosis on Testis in Mice

et al. 2015

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“…The height of the seminiferous epithelium was calculated by subtracting the lumen diameter from the tubule diameter. For each animal 150 tubules were analyzed [23,24].…”

Section: Morphometrymentioning

confidence: 99%

The effect of zinc oxide nanoparticles on mouse spermatogenesis

Talebi

Khorsandi

Moridian³

2013

J Assist Reprod Genet

126

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Objective To evaluate the effects of zinc oxide nanoparticles on mouse spermatogenesis. Methods Thirty two adult male NMRI mice were used. Experimental Groups (ZNP-1-ZNP-3) received one of the following treatments daily for 35 days: 5, 50 and 300 mg/kg zinc oxide nanoparticles respectively. Control group received only distilled water. Epididymal sperm parameters, testicular histopathology, morphometric analysis and spermatogenesis assessments were performed for evaluation of the zinc oxide nanoparticles effects on testis. Results Epididymal sperm parameters including sperm number, motility and percentage of abnormality were significantly changed in 50 and 300 mg/kg zinc oxide nanoparticles treated mice (p<0.01). Histopathological criteria such as epithelial vacuolization, sloughing of germ and detachment were significantly increased in 50 and 300 mg/kg zinc oxide nanoparticles treated mice (p<0.001). 300 mg/kg zinc oxide nanoparticles induced formation of multinucleated giant cells in the germinal epithelium. 50 and 300 mg/kg zinc oxide nanoparticles also caused a significant decrease in seminiferous tubule diameter, seminiferous epithelium height and maturation arrest (p<0.001). Conclusion Zinc oxide nanoparticles act as testicular toxicant and further studies are needed to establish its mechanism of action upon spermatogenesis.

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“…4) Presence of multinucleated giant cells within the lumen the seminiferous epithelium (Orazizadeh et al, 2010).…”

Section: Sperm Parameters Assessmentmentioning

confidence: 99%

Maternal Water Deprivation Affects the Spermatogenesis of the Offspring Rats

2013

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SUMMARY:Prenatal stresses such as water deprivation affect developmental process of embryo. This study evaluated the effects of water deprivation in pregnant mother on histological parameters of testis of offspring. Pregnant rats were divided into two groups (control and experimental). In experimental animals, water was removed from the ewes for 48h at the end of third trimester of gestation (19-21th days). Histopathology and histomorphic analysis and also TUNEL assay on offspring's testes were performed at pubertal age (60 days). The sperm motility either compared between two groups. The results showed that prenatal water deprivation induced histopathological alteration such as epithelium vacuolization, sloughing and detachments (P<0.01). Morphometrical data showed that prenatal water deprivation decreased tubular diameter and reduce epithelium height (P<0.01). Johnsen's score showed poor spermatogenesis in experimental group (P=0.001). The percent of germ cell apoptosis was increased in offspring's testes of rats born to mothers exposed to stress during pregnancy (P=0.000). The increased number of Multinucleated cells in the seminiferous lumen (P=0.000) in parallel with decreased number of sertoli cells (P=0.03) showed adverse effect of prenatal water deprivation on blood testis barrier. Present study revealed that prenatal water deprivation had injurious effect on developmental process of testes that affects on both germ cells and sertoli cells and had noxious effect on sperm parameters. These data confirm that prenatal stress disrupts normal spermatogenesis of offspring.

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Toxic effects of dexamethasone on mouse testicular germ cells

Cited by 59 publications

References 39 publications

Protective Effect of Tribulus terrestris Hydroalcoholic Extract Against CisplatinInduced Apoptosis on Testis in Mice

Protective Effect of Tribulus terrestris Hydroalcoholic Extract Against CisplatinInduced Apoptosis on Testis in Mice

The effect of zinc oxide nanoparticles on mouse spermatogenesis

Maternal Water Deprivation Affects the Spermatogenesis of the Offspring Rats

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Toxic effects of dexamethasone on mouse testicular germ cells

Cited by 59 publications

References 39 publications

Protective Effect of Tribulus terrestris Hydroalcoholic Extract Against Cisplatin­Induced Apoptosis on Testis in Mice

Protective Effect of Tribulus terrestris Hydroalcoholic Extract Against Cisplatin­Induced Apoptosis on Testis in Mice

The effect of zinc oxide nanoparticles on mouse spermatogenesis

Maternal Water Deprivation Affects the Spermatogenesis of the Offspring Rats

Contact Info

Product

Resources

About

Protective Effect of Tribulus terrestris Hydroalcoholic Extract Against CisplatinInduced Apoptosis on Testis in Mice

Protective Effect of Tribulus terrestris Hydroalcoholic Extract Against CisplatinInduced Apoptosis on Testis in Mice