Protective Effect of Tribulus terrestris Hydroalcoholic Extract Against Cisplatin­Induced Apoptosis on Testis in Mice

Abstract

SUMMARY:Cisplatin is an anti-cancer drug used in chemotherapy. One of the limiting side effects of cisplatin is decreasing genital gland function, azoospermia and oligospermia. Tribulus terrestris (TT) has been used as an aphrodisiac. The present study amid to investigate protective effect of TT hydroalcoholic extract against cisplatin-induced apoptosis on testis in mice. Male adult mice (n=30) were divided into control and 4 experimental groups (n=6). Control group received saline, first experimental group received cisplatin (5.5 mg/kg) and other three experimental group received cisplatin (5.5 mg/kg) and different doses of hydroalcoholic extact of TT (100, 300 and 500 mg/kg/i.p) respectively. Day after the last injection, histopathology and histomorphic analysis and also TUNEL assay on mice testis were performed. Weights of body and testis, seminiferous tubules diameter and apoptotic index were assessed. Data analysis was performed using one-way ANOVA followed by Tukeys' test. The results showed that cisplatin lead to a reduction in the weight of body and testes, and significantly increased apoptotic index compared to the control group (P<0.001), while in treated groups with TT, the weights of body and testis and seminiferous tubules diameter were significantly higher compared with cisplatin group (P<0.001), but apoptotic index did not show significant differences. The study demonstrates that extract of TT could protective effect of on cisplatin-induced apoptosis of testis and seminiferous tubules diameter that may be related to the presence of antioxidant components acting via a multitude of central and peripheral mechanisms

Tribulus terrestris Hydroalcoholic Extract Administration Effects on Reproductive Parameters and Serum Level of Glucose in Diabetic Male Rats

“…These data were similar to other studies (Shenoy & Ramesh;Suthagar et al, 2009;Kianifard et al, 2011). The weight gain in diabetic rats that were treated with T. terrestris confirmed Keshtmand et al (2015). The study showed that weight loss caused by diabetes can be compensated by T. terrestris extract first time.…”

“…The serum samples were then kept in deep freezer (-18 ºC) until measurement of testosterone hormone by ELISA method. Also, the testes were separated and weighed separately and then preserved in neutral buffered formalin 10 % (Keshtmand et al, 2015;Moradi et al, 2015).…”

“…To determine the motility, one drop of the sperm cells suspension was placed on the chamber and the motile and immotile sperm cells were analyzed by microscope with magnification 400X (Keshtmand et al, 2015).…”

Hepatoprotective Effects of Tribulus terrestris Hydro-Alcholic Extract on Non-Alcoholic Fatty Liver- Induced Rats

“…Further, administration of T. terrestris extract could significantly inhibit the gluconeogenesis and influence glycometabolism on normal mice and also reduces the level of triglyceride and the content of cholesterol in the plasma (Keshtmand et al, 2015). In previous works we showed the efficacy of T. terrestris extract on kidney injury induced by cisplatin and also side effects of diabetes on testis, sperm parameters and sexual hormones (Keshtmand et al, 2014;Keshtmand et al, 2015;Raoofi et al, 2015).…”

Tribulus terrestris Hydroalcoholic Extract Effect on Cisplatin-Induced Apoptosis in Mice Kidney

“…Then, fresh fruits of T. terrestris L were dried in shade at room temperature (25±2 ºC) and its extract was obtained through the percolati on method. Extracting method was described previously (Keshtmand et al, 2015). With this method, approximately 200 g of the dry plant specimen was ground and added to 400 mL of 70 % ethanol and were left to macerate at room temperature for 4 h. Then, the tinctured grains were extracted by percolation method and extract concentrated in a vacuum and dried in the flat surface.…”