Toxic effects of broflanilide exposure on development of zebrafish (Danio rerio) embryos and its potential cardiotoxicity mechanism

Duan, Manman; Zhang, Jie; Liu, Jia; Qian, Le; Chen, Xiangguang; Zhao, Feng; Zhao, Wentian; Zhong, Zhaomin; Yang, Yang; Wang, Chengju

doi:10.1016/j.envpol.2021.117481

Cited by 28 publications

(12 citation statements)

References 50 publications

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“…In the present study, a zebrafish larva with morphologically abnormal development did not have an inflated swim bladder even at 72 hph, which suggests that abnormal larvae may not achieve swim bladder inflation. In zebrafish larvae, abnormal development is often induced not only by thyroid hormone‐disrupting chemicals but also by exposure to various other chemicals, such as broflanilide (Duan et al, 2021), ethylparaben (Merola et al, 2020), fluxapyroxad (Lin et al, 2021), linuron (Maharaj et al, 2020), propylparaben (Perugini et al, 2019), 4‐epianhydrotetracycline (Wang et al, 2020), and chlorinated anilines (Horie, Yamagishi, et al, 2017), which induce edema, body curvature, or non‐inflation of the swim bladder. Taken together with these studies, our results indicate that when zebrafish are used for in vivo assays to detect thyroid hormone‐disrupting activity, the test period is shorter compared with the use of Japanese medaka, but to evaluate the effect of chemical substances on swim bladder inflation, it is necessary to determine whether the effect is due to thyroid disturbance or abnormal embryo development.…”

Section: Discussionmentioning

confidence: 99%

Effect of thyroid hormone‐disrupting chemicals on swim bladder inflation and thyroid hormone‐related gene expression in Japanese medaka and zebrafish

Horie

Nomura

Okamoto

et al. 2022

J of Applied Toxicology

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We compared the influence of thyroid hormone-disrupting chemicals (heptafluorobutanoic acid, PFBA and tris[1, phosphate, TDCPP) and thyroid hormone (3,3 0 ,5-triiodo-L-thyronine, T3) on swim bladder inflation and thyroid hormone-related gene expression in Japanese medaka and zebrafish. The swim bladder of most larvae had inflated at 4 h post hatching (hph) in Japanese medaka and at 48 hph in zebrafish in controls. In both fish species, the swim bladder inflation was inhibited in larvae exposed to PFBA (lowest observed effect concentration [LOEC] in medaka: 40 mg/L; in zebrafish: 80 mg/L), TDCPP (LOEC in medaka: 1 mg/L; in zebrafish: 0.5 mg/L), and T3 (no inhibition in Japanese medaka; LOEC in zebrafish: 7.5 μg/L). We also examined the influence of PFBA, TDCPP, and T3 on the expression of thyroid stimulating hormone subunit beta (tshβ) or thyroid hormone receptor alpha (trα) and beta (trβ). No changes were observed in the expression of genes after PFBA and TDCPP exposure; however, T3 exposure upregulated trα and trβ expression in both fish species. When the results were compared between Japanese medaka and zebrafish, swim bladder inflation in both species was found to be inhibited by exposure to thyroid hormone-disrupting chemicals. Our results show that inhibition of the swim bladder inflation at 4 hph in Japanese medaka and 48 hph in zebrafish is a potential indicator of thyroid hormone-disturbing activity of chemicals.

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Section: Discussionmentioning

confidence: 99%

Effect of thyroid hormone‐disrupting chemicals on swim bladder inflation and thyroid hormone‐related gene expression in Japanese medaka and zebrafish

Horie

Nomura

Okamoto

et al. 2022

J of Applied Toxicology

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“…Thus, it is important to clarify in advance the similarities, differences, and potential cross resistance between cyproflanilide and other commonly used insecticides. Broflanilide, which is chemically similar to cyproflanilide, has been reported to cause symptoms of toxicity in zebrafish such as reduced heart rate and shortened larval body length [29]. In FAW, sublethal doses of broflanilide can cause failure of ecdysis, reduced body length of larvae, malformation of pupae, and vestigial wing formation in adults [30].…”

Section: Discussionmentioning

confidence: 99%

The transcriptomic profile of Spodoptera frugiperda differs in response to a novel insecticide, cyproflanilide, compared to chlorantraniliprole and avermectin

et al. 2023

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Background Cyproflanilide is a novel chemical that is already undergoing insecticide registration in China and has been categorized as a member of group 30 by the IRAC. Since it was first detected in 2019, the fall armyworm (FAW), Spodoptera frugiperda, has become a serious pest in China. Our laboratory and field efficacy trials indicated that cyproflanilide exhibits high larvicidal activity against FAW. However, the effect of cyproflanilide against FAW remains unknown. And it is worth exploring further before the cyproflanilide becomes commercially available. Results We found larvae exposed to cyproflanilide had significantly shorter body length and higher death rates compared to control larvae. Additionally, we found surviving larvae had a significantly longer developmental period compared to control larvae. The potential molecular mechanisms of cyproflanilide against FAW were investigated using comparative transcriptomic analyses on larval samples subjected to three insecticide treatments, including cyproflanilide and two other commonly used insecticides against FAW in China, chlorantraniliprole and avermectin. We found that several subunits of the γ-aminobutyric acid receptor (GABAR), a possible target protein of cyproflanilide, were significantly up-regulated at the transcriptional level during cyproflanilide-induced stress. Additionally, between the control and cyproflanilide-treated samples, we identified 131 differentially expressed genes (DEGs) associated with detoxification metabolism. Of these, we found four P450 genes that were significantly up-regulated under cyproflanilide stress but were not DEGs when exposed to chlorantraniliprole and avermectin, or 23 other pesticides from previous reports. Furthermore, we discovered an interesting gene aggregation region for insect cuticle proteins (CPs) on the 18th chromosome, which is likely related to FAW cross-resistance to cyproflanilide and avermectin. Conclusions Our results contribute to a greater understanding of the mechanisms by which cyproflanilide affects FAW. Additionally, we identified the similarities and differences in transcriptomic profiling of FAW between the novel insecticide cyproflanilide and two other commonly used insecticides.

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“…The heart of zebrafish basically develops and has a regular heartbeat 48 h after fertilization. The heart rate change is an important factor to evaluate cardiotoxicity ( Duan et al, 2021 ). The pericardial region is widely used as a biomarker of zebrafish cardiotoxicity ( Mitchell et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Study on chemical constituents of Folium Artemisiae argyi Carbonisatum, toxicity evaluation on zebrafish and intestinal hemostasis

Wang

Shao

et al. 2022

Saudi Pharmaceutical Journal

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Toxic effects of broflanilide exposure on development of zebrafish (Danio rerio) embryos and its potential cardiotoxicity mechanism

Cited by 28 publications

References 50 publications

Effect of thyroid hormone‐disrupting chemicals on swim bladder inflation and thyroid hormone‐related gene expression in Japanese medaka and zebrafish

Effect of thyroid hormone‐disrupting chemicals on swim bladder inflation and thyroid hormone‐related gene expression in Japanese medaka and zebrafish

The transcriptomic profile of Spodoptera frugiperda differs in response to a novel insecticide, cyproflanilide, compared to chlorantraniliprole and avermectin

Study on chemical constituents of Folium Artemisiae argyi Carbonisatum, toxicity evaluation on zebrafish and intestinal hemostasis

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