Toxic chemicals-induced genotoxicity detected by random amplified polymorphic DNA (RAPD) in bean (Phaseolus vulgaris L.) seedlings

Cenkci, Süleyman; Yıldız, Mustafa; Çiğerci, İbrahim Hakkı; Konuk, Muhsin; Bozdağ, Ahmet

doi:10.1016/j.chemosphere.2009.05.001

Cited by 94 publications

(69 citation statements)

References 40 publications

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“…This variable correlations can be explained by a range of influencing factors, including the diversity among samples, variable reaction kinetics, presence and activity of enzymes responsible for mediating oxidative stress, (Jaio and Wang 2000;Wu et al 2004;Ozgen et al 2006) and etc. In the samples treated with Ni and Cd as non-redox metals, correspondingly to previously published facts we detected high levels of (Enan 2006;Kekec et al 2010;Cenkci et al 2009). The RAPD, PCR based assay described here is fast, reliable, and easy to conduct in any laboratory for assessment of environmental hazardous metals on plants.…”

Section: Resultssupporting

confidence: 87%

Mineral nutrient imbalance, total antioxidants level and DNA damage in common bean (Phaseolus vulgaris L.) exposed to heavy metals

Ackova

Panovska

Ruskovska

et al. 2013

Physiol Mol Biol Plants

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The present study aimed to analyze the biological effects induced by bioaccumulation of metals in common bean (Phaseolus vulgaris L.). Effects of mineral nutrient imbalance, total antioxidants level and DNA damage induced by accumulation of heavy metals, were investigated in bean seedlings treated with two selected metal concentrations for 7 days. Metal content is analyzed by inductively coupled plasma -atomic emission spectrometer (ICP-AES), for total antioxidants level assessment the Ferric-Reducing Antioxidant Power (FRAP) assay is used and Random Amplified Polymorphic DNA (RAPD) method was applied for investigation of DNA damages. The increasing metal concentration in the treatment medium changed synchronously metal content in samples, and decreased total antioxidant activity in all samples with exception only for samples treated with Ni and Cd. The obtained "DNA fingerprints" demonstrated that the increasing metal concentrations induced changes in RAPD profiles (disappearance and/or appearance of bands in comparison with untreated control samples). The highest number of missing bands was observed in samples treated with zinc (total 4 bands) and nickel (total 4 bands) at both concentrations. These results suggested that mineral nutrient imbalance is involved in changes of antioxidant levels and DNA damages of the seedlings, which may help to understand the mechanism of metal toxicity in plants.

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Section: Resultssupporting

confidence: 87%

Mineral nutrient imbalance, total antioxidants level and DNA damage in common bean (Phaseolus vulgaris L.) exposed to heavy metals

Ackova

Panovska

Ruskovska

et al. 2013

Physiol Mol Biol Plants

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“…A major source of the mutation load in living organisms is the oxidative DNA damages, with more than one hundred oxidative DNA adducts 8 . Recently, several selective and sensitive assays developed, such as restriction fragment length polymorphism, quantitative traits loci, random amplified polymorphic DNA (RAPD), amplified fragment length polymorphisms, simple sequence repeat, variable number of tandem repeats for DNA analysis in ecogenotoxicology as a result of the advances in molecular biology 9 . RAPD-PCR is one of the most feasible methods or techniques used for detection of DNA damage and mutations comparing DNA fingerprints from untreated and treated samples 10,11 .…”

Section: Introductionmentioning

confidence: 99%

Alterations in Biomarkers Associated with Sterility in Pectinophora gossypiella (Saunders) Induced by Gamma Irradiation

Ali

Moustafa

Sayed

2017

Braz. arch. biol. technol.

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“…This technique has been successfully used to study genotoxicity in different plants (Liu et al, 2005;Cenkci et al, 2009;Kumari et al, 2009;Liu et al, 2009). The lack of a published genomic DNA sequence for C. pepo prompted the use of this technique.…”

Section: Introductionmentioning

confidence: 99%

Random amplified polymorphic DNA reveals that TiO2 nanoparticles are genotoxic to Cucurbita pepo

Moreno-Olivas

Gant

Johnson

et al. 2014

J. Zhejiang Univ. Sci. A

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Titanium dioxide nanoparticles (TiO 2 NPs) are used in cosmetics, sunscreens, paints, and toothpaste, among other applications. These NPs are very stable and can be transported and dispersed in wastewater and biosolids. Animal species have shown negative reactions to TiO 2 NPs. However, little is known about their toxicity in plants, specifically the possibility of genotoxic effects. In this study, we used a random amplified polymorphic DNA (RAPD) technique to study the genotoxic effects of TiO 2 NPs on hydroponically cultivated zucchini (Cucurbita pepo) plants. Seeds were allowed to germinate for 7 d and plants were selected at random for individual and population studies. Four plants were selected for the individual study and 18 for the population study. RAPD profiles of TiO 2 NPs treated plants showed differences in band intensity, loss of bands, or appearance of new bands, compared to untreated plants. To the authors' knowledge, this is the first report of the genotoxic potential of TiO 2 NPs in zucchini.

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Toxic chemicals-induced genotoxicity detected by random amplified polymorphic DNA (RAPD) in bean (Phaseolus vulgaris L.) seedlings

Cited by 94 publications

References 40 publications

Mineral nutrient imbalance, total antioxidants level and DNA damage in common bean (Phaseolus vulgaris L.) exposed to heavy metals

Mineral nutrient imbalance, total antioxidants level and DNA damage in common bean (Phaseolus vulgaris L.) exposed to heavy metals

Alterations in Biomarkers Associated with Sterility in Pectinophora gossypiella (Saunders) Induced by Gamma Irradiation

Random amplified polymorphic DNA reveals that TiO2 nanoparticles are genotoxic to Cucurbita pepo

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