Random amplified polymorphic DNA reveals that TiO2 nanoparticles are genotoxic to Cucurbita pepo

Moreno-Olivas, Fabiola; Gant, Vincent U.; Johnson, Kyle L.; Peralta-Videa, José R.; Gardea‐Torresdey, Jorge L.

doi:10.1631/jzus.a1400159

Cited by 42 publications

(14 citation statements)

References 24 publications

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“…This is in contrast to Moreno-Olivas et al (2014) who observed n TiO 2 -induced genotoxicity in hydroponically cultivated zucchini. As the size of n TiO 2 they reported is comparable to that used in our work, the different results obtained can be explained by (i) the different cultivation systems (Petri dishes vs. full nutrient solution in hydroponics) and (ii) the n TiO 2 concentration used by Moreno-Olivas et al (2014 ; 10-fold smaller). The latter potentially prevents the formation of big NP agglomerates, making them more bioavailable.…”

Section: Discussioncontrasting

confidence: 99%

Evidence of Phytotoxicity and Genotoxicity in Hordeum vulgare L. Exposed to CeO2 and TiO2 Nanoparticles

et al. 2015

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Engineered nanoscale materials (ENMs) are considered emerging contaminants since they are perceived as a potential threat to the environment and the human health. The reactions of living organisms when exposed to metal nanoparticles (NPs) or NPs of different size are not well known. Very few studies on NPs–plant interactions have been published, so far. For this reason there is also great concern regarding the potential NPs impact to food safety. Early genotoxic and phytotoxic effects of cerium oxide NPs (nCeO2) and titanium dioxide NPs (nTiO2) were investigated in seedlings of Hordeum vulgare L. Caryopses were exposed to an aqueous dispersion of nCeO2 and nTiO2 at, respectively 0, 500, 1000, and 2000 mg l-1 for 7 days. Genotoxicity was studied by Randomly Amplified Polymorphism DNA (RAPDs) and mitotic index on root tip cells. Differences between treated and control plants were observed in RAPD banding patterns as well as at the chromosomal level with a reduction of cell divisions. At cellular level we monitored the oxidative stress of treated plants in terms of reactive oxygen species (ROS) generation and ATP content. Again nCeO2 influenced clearly these two physiological parameters, while nTiO2 were ineffective. In particular, the dose 500 mg l-1 showed the highest increase regarding both ROS generation and ATP content; the phenomenon were detectable, at different extent, both at root and shoot level. Total Ce and Ti concentration in seedlings was detected by ICP-OES. TEM EDSX microanalysis demonstrated the presence of aggregates of nCeO2 and nTiO2 within root cells of barley. nCeO2 induced modifications in the chromatin aggregation mode in the nuclei of both root and shoot cells.

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Section: Discussioncontrasting

confidence: 99%

Evidence of Phytotoxicity and Genotoxicity in Hordeum vulgare L. Exposed to CeO2 and TiO2 Nanoparticles

et al. 2015

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“…48 - 50 Yusefi-Tanha et al 51 found that small sized CuO nanoparticles (25 nm diameter) were more phytotoxic than large sized CuO nanoparticles (50 nm and 250 nm) to Soybean. Moreno-Olivas et al 52 reported that nanoparticles interaction with plant cell could lead to change in plant genes expression. Higher concentration of nanoparticles is toxic to plants as López-Moreno et al 53 reported that high dose of CeO 2 nanoparticles damaged the DNA structure in soybean.…”

Section: Discussionmentioning

confidence: 99%

Biosynthesis of ZnO Nanoparticles Using Bacillus Subtilis: Characterization and Nutritive Significance for Promoting Plant Growth in Zea mays L

et al. 2020

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Nano-fertilizer(s), an emerging field of agriculture, is alternate option for enhancement of plant growth replacing the synthetic fertilizers. Zinc oxide nanoparticles (ZnO NPs) can be used as the zinc source for plants. The present investigation was carried out to assess the role of ZnO NPs in growth promotion of maize plants. Biosynthesized ZnO NPs (using Bacillus sp) were characterized using Scanning Electron Microscope (SEM), Transmission Electron Microscope (TEM), X-ray diffraction (XRD) and Zeta potential. Different concentrations of ZnO NPs (2, 4, 8, 16 mg/L) were explored in pot culture experiment. Size of ZnO NPs ranged between 16 and 20 nm. A significant increase in growth parameters like shoot length (61.7%), root length (56.9%) and significantly higher level of protein was observed in the treated plants. The overall pattern for growth biomarkers including the protein contents was maximum at 8 mg/L of ZnO NPs. It was observed that application of biosynthesized ZnO NPs has improved majority of growth biomarkers including plant growth parameters, protein contents and leaf area. Therefore, biosynthesized ZnO NPs could be considered as an alternate source of nutrient in Zn deficient soils for promoting the modern agriculture.

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“…RAPD has been used to confirm the genotoxic effect of ZnO and CuO NP on buckwheat ( Fagopyrum esculentum ) seedlings ( Comrey and Lee, 2013 ). Additionally, RAPD analysis of zucchini ( C. pepo ) plants treated with TiO 2 NP exhibited DNA changes compared to the control plants ( Moreno-Olivas et al, 2014 ). Genotoxicity of CeO 2 and ZnO NP on soybean seedlings was demonstrated by the appearance of new DNA bands through RAPD analysis by López-Moreno et al (2010) .…”

Section: Discussionmentioning

confidence: 99%

Copper Nanoparticles Induced Genotoxicty, Oxidative Stress, and Changes in Superoxide Dismutase (SOD) Gene Expression in Cucumber (Cucumis sativus) Plants

et al. 2018

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With the increased use of metal nanoparticles (NPs), their access to the food chain has become a main concern to scientists and holds controversial social implications. This research particularly sheds light on copper nanoparticles (CuNP), as they have been commonly used in several industries nowadays. In this study, we investigated the phytotoxicity of CuNP on cucumber (Cucumis sativus) plants grown hydroponically. Atomic Absorption Spectroscopy (AAS), X-Ray Fluorescence (XRF), and Scanning Electron Microscopy (SEM) analysis confirmed that C. sativus treated with CuNP accumulated CuNP in the plant tissues, with higher levels in roots, with amounts that were concentration dependent. Furthermore, genotoxicity was assessed using Random amplified polymorphic DNA (RAPD) technique, and our results showed that CuNP caused genomic alterations in C. sativus. Phenotypical, physiological, and biochemical changes were assessed by determining the CuNP treated plant’s total biomass, chlorophyll, H2O2 and MDA contents, and electrolyte leakage percentage. The results revealed notable adverse phenotypical changes along with decreased biomass and decreased levels of the photosynthetic pigments (Chlorophyll a and b) in a concentration-dependent manner. Moreover, CuNP induced damage to the root plasma membrane as determined by the increased electrolyte leakage. A significant increase in H2O2 and MDA contents were detected in C. sativus CuNP treated plants. Additionally, copper-zinc superoxide dismutase (Cu-Zn SOD) gene expression was induced under CuNP treatment. Overall, our results demonstrated that CuNP of 10–30 nm size were toxic to C. sativus plants. This finding will encourage the safe production and disposal NPs. Thus, reducing nano-metallic bioaccumulation into our food chain through crop plants; that possesses a threat to the ecological system.

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Random amplified polymorphic DNA reveals that TiO2 nanoparticles are genotoxic to Cucurbita pepo

Cited by 42 publications

References 24 publications

Evidence of Phytotoxicity and Genotoxicity in Hordeum vulgare L. Exposed to CeO2 and TiO2 Nanoparticles

Evidence of Phytotoxicity and Genotoxicity in Hordeum vulgare L. Exposed to CeO2 and TiO2 Nanoparticles

Biosynthesis of ZnO Nanoparticles Using Bacillus Subtilis: Characterization and Nutritive Significance for Promoting Plant Growth in Zea mays L

Copper Nanoparticles Induced Genotoxicty, Oxidative Stress, and Changes in Superoxide Dismutase (SOD) Gene Expression in Cucumber (Cucumis sativus) Plants

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