Towards a smartphone-aided electronic ELISA for real-time electrochemical monitoring

Pechlivanidis, Nikolaos G.; Papadimitriou, Konstantinos I.; Evans, D. A.; Vasilakis, Nikolaos; Prodromakis, Themis

doi:10.1109/iscas.2017.8050616

Cited by 3 publications

(2 citation statements)

References 9 publications

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“…Recent integration of microfluidics [16][17][18], immunosensors [19][20][21][22][23], and plasmonic sensors [24][25][26][27][28][29] has enabled the development of promising point-of-care devices for disease detection.…”

Section: Introductionmentioning

confidence: 99%

Label-free detection of exosomes using a surface plasmon resonance biosensor

et al. 2019

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The development of a sensitive and specific detection platform for exosome is highly desirable as they are believed to transmit vital tumour-specific information (mRNAs, microRNAs and proteins) to the remote cells for secondary metastasis. Herein, we report a simple method for the real-time and label free detection of clinically relevant exosomes using surface plasmon resonance (SPR) biosensor. Our method shows high specificity in detecting BT474 breast cancer cell derived exosomes particularly from complex biological samples (e.g., exosome spiked in serum). This approach exhibits high sensitivity by detecting as low as 8280 exosomes/µL which may potentially be suitable for clinical analysis. We believe that this label free and real time method along with the high specificity and sensitivity may potentially be useful for clinical settings.

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Section: Introductionmentioning

confidence: 99%

Label-free detection of exosomes using a surface plasmon resonance biosensor

et al. 2019

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“…Such integration inevitably favors the development of PoC platforms with smaller footprint and consequently development cost [ 46 ]. Combining both biochemistry and electronic biosensing on the same sensing platform may also reduce noise interference due to connectivity issues which could increase the signal-to-noise ratio (SNR) of the measurement [ 47 ].…”

Section: Introductionmentioning

confidence: 99%

A Novel Microfluidic Point-of-Care Biosensor System on Printed Circuit Board for Cytokine Detection

Evans

Papadimitriou

Vasilakis

et al. 2018

Sensors

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Point of Care (PoC) diagnostics have been the subject of considerable research over the last few decades driven by the pressure to detect diseases quickly and effectively and reduce healthcare costs. Herein, we demonstrate a novel, fully integrated, microfluidic amperometric enzyme-linked immunosorbent assay (ELISA) prototype using a commercial interferon gamma release assay (IGRA) as a model antibody binding system. Microfluidic assay chemistry was engineered to take place on Au-plated electrodes within an assay cell on a printed circuit board (PCB)-based biosensor system. The assay cell is linked to an electrochemical reporter cell comprising microfluidic architecture, Au working and counter electrodes and a Ag/AgCl reference electrode, all manufactured exclusively via standard commercial PCB fabrication processes. Assay chemistry has been optimised for microfluidic diffusion kinetics to function under continual flow. We characterised the electrode integrity of the developed platforms with reference to biological sampling and buffer composition and subsequently we demonstrated concentration-dependent measurements of H2O2 depletion as resolved by existing FDA-validated ELISA kits. Finally, we validated the assay technology in both buffer and serum and demonstrate limits of detection comparable to high-end commercial systems with the addition of full microfluidic assay architecture capable of returning diagnostic analyses in approximately eight minutes.

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