Towards a better testicular sperm extraction: novel sperm sorting technologies for non-motile sperm extracted by microdissection TESE

Mangum, Cathy L; Patel, Darshan P.; Jafek, Alexander; Samuel, Reichel; Jenkins, Timothy G.; Aston, Kenneth I.; Gale, Bruce K.; Hotaling, James M.

doi:10.21037/tau.2019.08.36

Cited by 24 publications

(21 citation statements)

References 30 publications

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“…Whereas in secondary form, non-surgical treatment may be a viable option, in primary one, surgical sperm retrieval is the main treatment. There are various sperm retrieval techniques with their own advantage and disadvantage (13). (E.g.…”

Section: Introductionmentioning

confidence: 99%

Success rate and ART outcome of microsurgical sperm extraction in non obstructive azoospermia: A retrospective study

Vahidi¹,

Horoki²,

Talkhooncheh³

et al. 2021

IJRM

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Background: The management of non-obstructive azoospermia (NOA) disease relies on microdissection testicular sperm extraction (micro-TESE). Few studies have assessed the role of micro-TESE in men with NOA in our country. Objective: The aim of the current study was to investigate the success rate of micro- TESE. Materials and Methods: This retrospective descriptive-analytical study was conducted on 463 men with NOA in Yazd Reproductive Sciences Institute during September 2017 through September 2019. Sperm were retrieved and frozen according to the rapid sperm freezing protocol. After preparing the oocyte of the male partner’s spouse, sperms were thawed and then entered the intracytoplasmic sperm injection process. The clinical pregnancy of individuals was confirmed via ultrasound. Demographic data were extracted from medical records. Results: The success rate of micro-TESE was 38% and successful fertilization, biochemical pregnancy, clinical pregnancy, and live birth were observed in 111 (85.4%), 29 (22.3%), 29 (22.3%) and 14 (10.7%) men, respectively. A significant difference was seen between the two groups, regarding age (p = 0.01). In addition, the mean follicle-stimulating hormone in men with positive micro-TESE was significantly lower than in men with negative micro-TESE (p = 0.02). Conclusion: The success of pregnancy in couples with NOA managed via micro-TESE was significant. The study found that the success rate of micro-TESE was higher in older men and in those with lower follicle-stimulating hormone levels. Key words: Azoospermia, Fertilization, Microdissection, Testicular.

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Section: Introductionmentioning

confidence: 99%

Success rate and ART outcome of microsurgical sperm extraction in non obstructive azoospermia: A retrospective study

Vahidi¹,

Horoki²,

Talkhooncheh³

et al. 2021

IJRM

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“…In a pilot study, testicular spermatozoa could be isolated using this technique in cases of NOA where sperm recovery could not be achieved in previous TESE attempts [ 121 ]. However, alterations in cell viability due to fluorophores and antibodies, the cost of the system, high cell loss, and its time-consuming nature are potential limiting factors for the use of FACS in testicular cell selection for ICSI [ 122 ].…”

Section: Processing and Selection Of Surgically Retrieved Sperm For Icsimentioning

confidence: 99%

Sperm Selection Procedures for Optimizing the Outcome of ICSI in Patients with NOA

Aydos

2021

JCM

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Retrieving spermatozoa from the testicles has been a great hope for patients with non-obstructive azoospermia (NOA), but relevant methods have not yet been developed to the level necessary to provide resolutions for all cases of NOA. Although performing testicular sperm extraction under microscopic magnification has increased sperm retrieval rates, in vitro selection and processing of quality sperm plays an essential role in the success of in vitro fertilization. Moreover, sperm cryopreservation is widely used in assisted reproductive technologies, whether for therapeutic purposes or for future fertility preservation. In recent years, there have been new developments using advanced technologies to freeze and preserve even very small numbers of sperm for which conventional techniques are inadequate. The present review provides an up-to-date summary of current strategies for maximizing sperm recovery from surgically obtained testicular samples and, as an extension, optimization of in vitro sperm processing techniques in the management of NOA.

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“…In addition, sperm from these samples often have different morphologic abnormalities. Applications for purifying testicular sperm are currently being developed (46,48). Hopefully, microfluidics and/or other approaches to automation will have the potential to improve the speed and efficiency of purifying rare sperm in order to rapidly identify high-quality sperm for ICSI, thus replacing the extremely time consuming and labor intensive manual procedure.…”

Section: Essential Pointsmentioning

confidence: 99%

“…The authors reported that this method was potentially useful for treating raw semen and sorting rare sperm samples. Even sperm obtained by testicular sperm extraction can be isolated in this way (48). De Wagenaar et al (62) demonstrated that boar sperm could be separated on a dielectrophoretic field based on a morphologic anomaly, cytoplasmic droplets on the flagellum.…”

Section: Essential Pointsmentioning

confidence: 99%

Are we approaching automated assisted reproductive technology? Sperm analysis, oocyte manipulation, and insemination

Casciani¹,

Galliano

Franasiak

et al. 2021

F&S Reviews

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Assisted reproductive technology (ART) procedures are mostly performed manually and require meticulous attention to details and precision in handling and timing. Automation of ART procedures would allow achieving standardization and reducing performance variability, the cost of medical treatment, and the risk of human error. Some ART diagnostic devices are already available and offer objective tools of evaluation. However, automation of the entire spectrum of ART procedures is yet to come and can only be imagined as a platform capable of integrating all the separate technologies, successfully interconnecting them to guarantee a continued chain of custody of the gametes and embryos. The present update seeks to review the current potential for automation within the in vitro fertilization laboratory, with attention to sperm and oocyte manipulation and selection and to oocyte insemination with standard in vitro fertilization or intracytoplasmic sperm injection. An electronic search of PubMed was performed to identify articles in English that addressed automation in ART. Studies were classified in categories as randomized controlled trials, prospective controlled trials, prospective noncontrolled trials, retrospective studies, and experimental studies. Research and development data from the authors are included.

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Towards a better testicular sperm extraction: novel sperm sorting technologies for non-motile sperm extracted by microdissection TESE

Cited by 24 publications

References 30 publications

Success rate and ART outcome of microsurgical sperm extraction in non obstructive azoospermia: A retrospective study

Success rate and ART outcome of microsurgical sperm extraction in non obstructive azoospermia: A retrospective study

Sperm Selection Procedures for Optimizing the Outcome of ICSI in Patients with NOA

Are we approaching automated assisted reproductive technology? Sperm analysis, oocyte manipulation, and insemination

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