Sperm Selection Procedures for Optimizing the Outcome of ICSI in Patients with NOA

Aydos, Kaan; Aydos, Oya Sena

doi:10.3390/jcm10122687

Cited by 19 publications

(18 citation statements)

References 264 publications

(313 reference statements)

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“…According to the World Health Organization laboratory manual for the examination and processing of human semen [ 23 ], HOST is advised in cases of asthenozoospermia to collect suitable spermatozoa for ICSI. Some studies suggested that HOST should be employed in a subsequent ICSI cycle following total fertilization failure [ 80 ].…”

Section: Advanced Methodsmentioning

confidence: 99%

Sperm Selection for ICSI: Do We Have a Winner?

Baldini

Ferri

Baldini

et al. 2021

Cells

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In assisted reproductive technology (ART), the aim of sperm cells’ preparation is to select competent spermatozoa with the highest fertilization potential and in this context, the intracytoplasmic sperm injection (ICSI) represents the most applied technique for fertilization. This makes the process of identifying the perfect spermatozoa extremely important. A number of methods have now been developed to mimic some of the natural selection processes that exist in the female reproductive tract. Although many studies have been conducted to identify the election technique, many doubts and disagreements still remain. In this review, we will discuss all the sperm cell selection techniques currently available for ICSI, starting from the most basic methodologies and continuing with those techniques suitable for sperm cells with reduced motility. Furthermore, different techniques that exploit some sperm membrane characteristics and the most advanced strategy for sperm selection based on microfluidics, will be examined. Finally, a new sperm selection method based on a micro swim-up directly on the ICSI dish will be analyzed. Eventually, advantages and disadvantages of each technique will be debated, trying to draw reasonable conclusions on their efficacy in order to establish the gold standard method.

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Section: Advanced Methodsmentioning

confidence: 99%

Sperm Selection for ICSI: Do We Have a Winner?

Baldini

Ferri

Baldini

et al. 2021

Cells

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“…Like the HOS test, STFT and LAISS could give false positives for frozen sperm as the flagellum could spontaneously curl after freezing-thawing [ 53 54 ]. These two techniques, STFT and LAISS, have not been described in recent years, probably because their costs in terms of learning time (STFT) or in terms of equipment (LAISS) might limit their routine use in ART laboratories [ 53 54 57 ].…”

Section: Management Of Necrozoospermiamentioning

confidence: 99%

“…Therefore, there are several techniques to test the vitality of immobile spermatozoa. Most studies have focused on activating substances such as pentoxifylline (PTX) or theophylline describing their role in activating flagellar movement of immotile spermatozoa (for a review see [ 53 54 57 ]). PTX is a 3′ 5′ nucleotidase phosphodiesterase inhibitor that improves sperm motility by increasing intracellular cyclic adenosine monophosphate [ 58 ].…”

Section: Management Of Necrozoospermiamentioning

confidence: 99%

“…Theophylline, which belongs to the same family, has been studied less frequently since its first clinical trial in 2011 [ 60 ]. Since then, several studies have shown the effectiveness of these substances in inducing sperm motility in immotile but alive spermatozoa (for a review see [ 54 57 ]). Their safety has been demonstrated in several studies (for a review see [ 53 54 57 ]).…”

Section: Management Of Necrozoospermiamentioning

confidence: 99%

See 1 more Smart Citation

Sperm Vitality and Necrozoospermia: Diagnosis, Management, and Results of a Global Survey of Clinical Practice

Agarwal

Sharma

Gupta

et al. 2022

World J Mens Health

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Sperm vitality testing is a basic semen examination that has been described in the World Health Organization (WHO) Laboratory Manual for the Examination and Processing of Human Semen from its primary edition, 40 years ago. Several methods can be used to test sperm vitality, such as the eosin-nigrosin (E-N) stain or the hypoosmotic swelling (HOS) test. In the 6th (2021) edition of the WHO Laboratory Manual, sperm vitality assessment is mainly recommended if the total motility is less than 40%. Hence, a motile spermatozoon is considered alive, however, in certain conditions an immotile spermatozoon can also be alive. Therefore, the differentiation between asthenozoospermia (pathological decrease in sperm motility) and necrozoospermia (pathological decrease in sperm vitality) is important in directing further investigation and management of infertile patients. The causes leading to necrozoospermia are diverse and can either be local or general, testicular or extra-testicular. The andrological management of necrozoospermia depends on its etiology. However, there is no standardized treatment available presently and practice varies among clinicians. In this study, we report the results of a global survey to understand current practices regarding the physician order of sperm vitality tests as well as the management practices for necrozoospermia. Laboratory and clinical scenarios are presented to guide the reader in the management of necrozoospermia with the overall objective of establishing a benchmark ranging from the diagnosis of necrozoospermia by sperm vitality testing to its clinical management.

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“…The outcome of mTESE is greatly affected by the accuracy in testicular sperm processing techniques. Kaan Aydos and Oya Sena Aydos reviewed the available sperm selection procedures, as well as the different approaches to testicular sperm cryopreservation, providing valuable suggestions for embryologists and clinicians about how to effectively handle testicular specimens and testicular sperm to maximize the outcome of mTESE [ 11 ].…”

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confidence: 99%