Toward optimized antibody microarrays: a comparison of current microarray support materials

Angenendt, Philipp; Glökler, Jörn; Murphy, Derek; Lehrach, Hans; Cahill, Dolores J.

doi:10.1016/s0003-2697(02)00257-9

Cited by 304 publications

(191 citation statements)

References 12 publications

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“…HydroGel slides performed best with regard to detection limit, but showed greater variations than nongel-coated slides. Hydrogel slides were demonstrated with CV of 11-37% for the intrafield analysis and 15-60% for the interfiled analysis [12]. In contrast, in our experiment, the CV for the intra- array and the interarray were 1.3-5.3% and 8.7-39% respectively, at different capture antibody concentrations.…”

Section: Reliability Of the Systemcontrasting

confidence: 70%

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Construction of an antibody microarray based on agarose‐coated slides

Liu

Zhang

et al. 2007

Electrophoresis

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Construction of an antibody microarray based on agarose-coated slidesThe antibody microarray, a high-throughput multiplex immunoassay method, has become a significant tool for quantitative proteomics studies. We describe here the strategies for optimizing the condition of antibody microarray building based on agarose-coated slides. In this study, modified glass slides were robotically printed with capture antibodies against monocyte chemoattractant protein 1 (MCP-1), then dilutions of the cytokine were applied to the arrays, and the protein was detected with biotin-labeled antibody coupled with Cy3-conjugated streptavidin. Thus a protein profiling microarray based on sandwich immunoassay has been established. Various factors in the production of antibody microarrays were analyzed: the capture antibody concentrations, shelf life of the postprinting slides, blocking buffers, and reproducibility of the system. A calibration curve with a correlation coefficient of 0.9995 was established which suggested that the matrix can retain arrayed proteins in near-quantitative fashion. The results revealed high signal uniformity and reproducibility with regard to intra-array (1.3%) and the interarray (8.7%) variation at the capture antibody concentration of 125 mg/mL. Besides, the printed arrays could be stored for at least two months without any apparent change of the performance parameters.

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Section: Reliability Of the Systemcontrasting

confidence: 70%

“…Angenendt et al [12] screened 11 different array surfaces including plain nongel-coated glass or plastic surfaces and gel-coated surfaces (self-assembled polyacrylamidecoated slides and HydroGel slides). HydroGel slides performed best with regard to detection limit, but showed greater variations than nongel-coated slides.…”

Section: Reliability Of the Systemmentioning

confidence: 99%

Construction of an antibody microarray based on agarose‐coated slides

Liu

Zhang

et al. 2007

Electrophoresis

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show abstract

“…The ultimate goal of developing this technology is to construct ordered arrays of individual proteins for biochemical study at the molecular level. A number of different sources of peptides and proteins have been used for protein microarray manufacture, including synthetic peptides (7,8), recombinant proteins (9 -11), and monoclonal and polyclonal antibodies (12)(13)(14). Microarrays have been utilized to study protein expression profiles (15)(16)(17), protein-protein interactions (18,19), drug analysis (11,20), and the diagnosis of diseases such as cancer, food allergies, and infection by pathogenic viruses and bacteria (21)(22)(23)(24).…”

mentioning

confidence: 99%

Protein Expression Profiling of Breast Cancer Cells by Dissociable Antibody Microarray (DAMA) Staining

Song

Yang

et al. 2008

Molecular & Cellular Proteomics

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“…PLL provides a very flexible substrate for many different applications including classical cDNA applications, as well as the immobilization of oligonucleotides [58], peptides and proteins [59,60], antigens [61], and antibodies [62,63]. PLL slides were successfully applied as a solid support of a siRNA microarray used for cell transfection [64].…”

Section: Molecular Architecturementioning

confidence: 99%

Microarray Technology as a Universal Tool for High-Throughput Analysis of Biological Systems

Sobek¹,

Bartscherer²,

Jacob³

et al. 2006

CCHTS

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109

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Over the last years microarray technology has become one of the principal platform technologies for the highthroughput analysis of biological systems. Starting with the construction of first DNA microarrays in the 1990s, microarray technology has flourished in the last years and many different new formats have been developed. Peptide and protein microarrays are now applied for the elucidation of interaction partners, modification sites and enzyme substrates. Antibody microarrays are envisaged to be of high importance for the high-throughput determination of protein abundances in translational profiling approaches. First cell microarrays have been constructed to transform microarray technology from an in vitro technology to an in vivo functional analysis tool. All of these approaches share a common prerequisite: the solid support on which they are generated. The demands on this solid support are thereby as manifold as the applications themselves. This review is aimed to display the recent developments in surface chemistry and derivatization, and to summarize the latest developments in the different application areas of microarray technology.

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Toward optimized antibody microarrays: a comparison of current microarray support materials

Cited by 304 publications

References 12 publications

Construction of an antibody microarray based on agarose‐coated slides

Construction of an antibody microarray based on agarose‐coated slides

Protein Expression Profiling of Breast Cancer Cells by Dissociable Antibody Microarray (DAMA) Staining

Microarray Technology as a Universal Tool for High-Throughput Analysis of Biological Systems

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