Protein Expression Profiling of Breast Cancer Cells by Dissociable Antibody Microarray (DAMA) Staining

Song, Xi; Fu, Guanyuan; Yang, Xufen; Jiang, Zhong; Wang, Yingjian; Zhou, G.T.

doi:10.1074/mcp.m700115-mcp200

Cited by 36 publications

(30 citation statements)

References 31 publications

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“…Our data are in full agreement with in vitro studies that compared TYK2 levels in different breast cancer cell lines and found them to be higher in ERα(+) cell lines (T47D, MCF7, ZR-75-1) ) when compared to ERα(−) lines (MDA-MB-231, BT549, HS578T, MDA-MB-435S), and immortalized normal lines (MCF10A, MCF12A, and Hs578Bst) [31]. Interestingly, among all breast cancer cell lines analyzed in the latter study, TYK2 levels were the lowest in BT549, a cell line derived from an invasive ductal tumor that had metastasized to 3 of 7 regional lymph nodes [31]. …”

Section: Discussionsupporting

confidence: 90%

Non-receptor tyrosine kinase 2 reaches its lowest expression levels in human breast cancer during regional nodal metastasis

Sang

Man

Sung

et al. 2011

Clin Exp Metastasis

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Almost half of breast Ductal Carcinoma in situ are likely to remain non threatening in situ lesions with no invasion to the surrounding stroma and no metastases. The majority of focal disruptions in myoepithelial (ME) cell layers indicative of invasion onset were found to be overlying epithelial cell clusters with no or substantially reduced estrogen receptor α (ERα) expression. Here we report the down-regulation of Tyrosine kinase-2 (TYK2) and up-regulation of Strumpellin expression, among other proteins in ERα(−) cells located at disrupted ME layers compared to adjacent ERα(+) cells overlying an intact myoepithelial layer. ERα(+) and ERα(−) cells were microdissected from the same in vivo human breast cancer tissues, proteins were extracted and separated utilizing Differential in-Gel Electrophoresis (DIGE) followed by trypsin digestion, MALDI-TOF analysis, and protein identification. Proteins expressed by ERα(−) cell clusters were found to express higher levels of strumpellin that binds to Valosin-containing protein (VCP) to slow-down wound closure and promote growth; and lower levels of TYK2, a jak protein necessary for lineage specific differentiation. TYK2 levels were further analyzed by immunohistochemistry (IHC) in a cohort composed of 70 patients with broad clinical characteristics. TYK2 levels were minimal in TxN1M0 breast cancers which is the stage where the initial regional lymph node metastasis is observed. Our data highlight the role of TYK2 downregulation in breast cancer cell de-differentitation and initiation of regional metastasis. In addition, the aggressiveness of the ERα(−) cell clusters compared to ERα(+) ones present in the same duct of the same patient was confirmed.

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Section: Discussionsupporting

confidence: 90%

Non-receptor tyrosine kinase 2 reaches its lowest expression levels in human breast cancer during regional nodal metastasis

Sang

Man

Sung

et al. 2011

Clin Exp Metastasis

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“…Contrasting data were recently found by a dissociable antibody microarray (DAMA), a technique that combines protein microarrays with traditional immunostaining, on normal and cancer breast cell lines showing that p107 is one of the upregulated proteins in cancer compared to normal cells (119). Data were confirmed by Western blot analysis and statistical analysis leading the authors to consider p107 a candidate biomarker for breast cancer diagnosis.…”

Section: P107supporting

confidence: 50%

Rb family proteins in gastric cancer (Review)

Cito¹,

Pentimalli

Forte

et al. 2010

Oncol Rep

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Abstract. Gastric cancer is one of the most diffuse neoplastic pathologies in the world whose environmental and molecular causes, although deeply investigated, have not been completely clarified. Besides some well-established etiological factors, such as Helicobacter pylori and E-cadherin mutations, investigations on other possible causes gave contrasting results. Rb family proteins (including pRb/p105, pRb2/p130 and p107) are involved in cell cycle regulation and their function and/or expression is often lost in various kinds of tumours such as lung, bladder, breast and brain cancer. The consequences of RB inactivation in tumours can be very different depending on the context and the type of cancer. Recent evidence indicates that Rb status correlates with a different therapeutic response according to the tumour type and the therapeutic agent. Studies performed on Rb family proteins in gastrointestinal tract tumours suggest that these proteins have an important role in these cancer types. However, owing to contrasting results, further investigation is required to assess whether the expression of Rb family proteins can potentially be used as a prognostic or predictive factor in gastric cancer.

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“…Tomasson and colleagues describe a potential association of Tyk2 germ-line sequence changes to acute myelogenous leukemia pathogenesis. Elevated Tyk2 expression levels detected in breast cancer cell lines prompted the authors to propose Tyk2 as a potential biomarker for breast cancer diagnosis (22). Moreover, loss of Tyk2 expression and signaling has been shown to inhibit the invasiveness of human prostate cancer cells (23) and of leukemic B cells in a murine model system of human Burkitt's lymphoma (24).…”

Section: Introductionmentioning

confidence: 99%

Identification of an Indispensable Role for Tyrosine Kinase 2 in CTL-Mediated Tumor Surveillance

et al. 2008

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We showed previously that Tyk2 À/À natural killer cells lack the ability to lyse leukemic cells. As a consequence, the animals are leukemia prone. Here, we show that the impaired tumor surveillance extends to T cells. Challenging Tyk2 À/À mice with EL4 thymoma significantly decreased disease latency. The crucial role of Tyk2 for CTL function was further characterized using the ovalbumin-expressing EG7 cells. Tyk2 À/À OT-1 mice developed EG7-induced tumors significantly faster compared with wild-type (wt) controls. In vivo assays confirmed the defect in CD8

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Protein Expression Profiling of Breast Cancer Cells by Dissociable Antibody Microarray (DAMA) Staining

Cited by 36 publications

References 31 publications

Non-receptor tyrosine kinase 2 reaches its lowest expression levels in human breast cancer during regional nodal metastasis

Non-receptor tyrosine kinase 2 reaches its lowest expression levels in human breast cancer during regional nodal metastasis

Rb family proteins in gastric cancer (Review)

Identification of an Indispensable Role for Tyrosine Kinase 2 in CTL-Mediated Tumor Surveillance

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