Microarray Technology as a Universal Tool for High-Throughput Analysis of Biological Systems

Abstract

Over the last years microarray technology has become one of the principal platform technologies for the highthroughput analysis of biological systems. Starting with the construction of first DNA microarrays in the 1990s, microarray technology has flourished in the last years and many different new formats have been developed. Peptide and protein microarrays are now applied for the elucidation of interaction partners, modification sites and enzyme substrates. Antibody microarrays are envisaged to be of high importance for the high-throughput determination of protein abundances in translational profiling approaches. First cell microarrays have been constructed to transform microarray technology from an in vitro technology to an in vivo functional analysis tool. All of these approaches share a common prerequisite: the solid support on which they are generated. The demands on this solid support are thereby as manifold as the applications themselves. This review is aimed to display the recent developments in surface chemistry and derivatization, and to summarize the latest developments in the different application areas of microarray technology.

Functionalizable low-fouling coatings for label-free biosensing in complex biological media: advances and applications

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Large-Scale Protein-Protein Interaction Detection Approaches: Past, Present and Future

“…Using interaction domains for PPI studies is justifi ed by the fact that protein binding domains are usually restricted to short amino acid sequences. Thus, domains or synthetic peptides representing a protein binding site often show the same biological activity as the protein itself (88). However, it may be possible that the exposition of the entire domain outside of its native protein context may open additional interaction possibilities not normally observed for the protein in question.…”

An equipment-free polydimethylsiloxane microfluidic spotter for fabrication of microarrays

“…1 They enable rapid, highly parallel analysis of target molecules in an unknown sample. A microarray-based analytical strategy is quicker and more convenient than serial testing for individual analytes, and it has been successfully applied to a variety of biological assays, such as sequencing by hybridization, 2 mutation detection, 3 assessment of gene copy number, 4 comparative genome hybridization, 5 drug discovery, 6 expression analysis, 7 immunoassays, 8 and proteomic analysis.…”