Toward fingerprinting cellular lipidomes directly from biological samples by two-dimensional electrospray ionization mass spectrometry

Han, Xianlin; Yang, Jingyue; Cheng, Hua; Ye, Hongping; Gross, Richard W.

doi:10.1016/j.ab.2004.04.004

Cited by 212 publications

(138 citation statements)

References 34 publications

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“…Precursor ion scanning (PIS) and neutral loss scanning (NLS) for head group survey (HGS) and fatty acid survey (FAS), respectively, are typically performed in the positive or negative ion modes. PIS and NLS of the polar head groups of PLs are valuable techniques for detecting most molecular species within the same class of PLs [29][30][31][32][33][34][35]. PIS of carbonic anions was also very useful for identifying molecular species of PLs with specified fatty acyl chains as reported by Ekroos et al [29].…”

Section: Analytical Systems By Mass Spectrometry In Lipidomics J235mentioning

confidence: 82%

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Lipidomics for Elucidation of Metabolic Syndrome and Related Lipid Metabolic Disorder

2012

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Lipidomics is an essential technique to elucidate the recent diseases such as metabolic syndrome in which lipid metabolic disorder is considered as one of the main physiological changes [1][2][3][4]. Among them, lipid oxidation is the most prominent event as a result of oxidative stress that is thought to be a major physiological process occurring in the progress of these diseases during aging of the human life [5][6][7]. Thus, a basic analytical system is very important especially for the analysis of the distribution of polyunsaturated fatty acid (PUFA) containing lipids in several organs where the increase in and the existence of their oxidation and accumulation of their oxidized metabolites such as hydroperoxides, hydrooxides, ketons, epoxides, aldehydes, and carboxylated species are expected.Sources of PUFA in mammalian tissues are exclusively regulated by external factors, especially food supply. Thus, both qualitative and quantitative states of lipid supply from foods for mammalians are extremely important to the progress of these diseases resulting from lipid metabolic disorder including lipid oxidation.Recent progress of lipidomics has made it possible to know exact distribution of lipid molecular species containing saturated, monounsaturated, and polyunsaturated fatty acids (SFA, MUFA, and PUFA) even in the states of individual classes in phospholipids (PLs) and triglycerides (TGs) [8][9][10][11][12]. Also, their specified localizations are proved to reflect physiological functions of these molecular species within different individual organs and tissue regions.Molecular species of PLs in individual organs have been proved to be strictly regulated by localization of phospholipases, acyltransferases, and acyl-CoA concentration specifically regulated by acyl-CoA synthetases, fatty acid elongases, and desaturases. These facts have been revealed recently by the application of global analysis by mass spectrometry (MS). Recent methods in lipidomics by mass spectrometry have been very rapidly progressing, making global and comprehensive analyses of individual lipid molecular species in tissues possible [8][9][10][11][12]. As a result, Lipidomics, First Edition. Edited by Kim Ekroos.

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Section: Analytical Systems By Mass Spectrometry In Lipidomics J235mentioning

confidence: 82%

“…[29][30][31][32][33][34][35] There are two types of structure-related focused analysis by ESIMS/MS. Precursor ion scanning (PIS) and neutral loss scanning (NLS) for head group survey (HGS) and fatty acid survey (FAS), respectively, are typically performed in the positive or negative ion modes.…”

Section: Analytical Systems By Mass Spectrometry In Lipidomics J235mentioning

confidence: 99%

Lipidomics for Elucidation of Metabolic Syndrome and Related Lipid Metabolic Disorder

2012

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“…Identification of A-type ceramides in the stratum corneum of human skin Direct infusion of lipid extracts into a tandem mass spectrometer has been used for ceramide profiling in various samples such as mouse liver [27], rat brain tissues and cerebral endothelial cells [25], human plasma [28], and human skin fibroblasts cell [29]. In our previous study, this method was applied to N-type ceramide analysis in the skin stratum corneum [24].…”

Section: General Ms/ms Fragmentation Pattern Of A-type Ceramidesmentioning

confidence: 99%

Lipidomic platform for structural identification of skin ceramides with α-hydroxyacyl chains

Shon

Lee

et al. 2016

Anal Bioanal Chem

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Skin ceramides are sphingolipids consisting of sphingoid bases, which are linked to fatty acids via an amide bond. Typical fatty acid acyl chains are composed of α-hydroxy fatty acid (A), esterified ω-hydroxy fatty acid (EO), non-hydroxy fatty acid (N), and ω-hydroxy fatty acid (O). We recently established a lipidomic platform to identify skin ceramides with non-hydroxyacyl chains using tandem mass spectrometry. We expanded our study to establish a lipidomic platform to identify skin ceramides with α-hydroxyacyl chains. Tandem mass spectrometry analysis of A-type ceramides using chip-based direct infusion nanoelectrospray-mass spectrometry showed the characteristic fragmentation pattern of both acyl and sphingoid units, which can be applied for structural identification of ceramides. Based on the tandem mass spectrometry fragmentation patterns of A-type ceramides, comprehensive fragmentation schemes were proposed. Our results may be useful for identifying A-type ceramides in the stratum corneum of human skin.

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“…Electrospray ionization mass spectrometry enables the identification and quantification of the cellular or tissue lipidomes directly from crude extracts of cell, tissue or organ samples [21][22][23] . The mass spectrometers that resolve fragments of entrant precursor ions of biomolecules in space domains enable identification and quantification in a class specific manner, referred to as automated shotgun lipid profiling [24] .…”

Section: Class Specific Analyses Of Lipids On Mass Analyzer Where Framentioning

confidence: 99%

Lipidomic mass spectrometry and its application in neuroscience

Enriquez-Algeciras¹,

Bhattacharya²

2013

WJBC

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can be regarded to as mass analyzers. There are those that separate and analyze the product ion fragments in space (spatial) and those which separate product ions in time in the same space (temporal). Databases and standardized instrument parameters have further aided the capabilities of the spatial instruments while recent advances in bioinformatics have made the identification and quantification possible using temporal instruments.© 2013 Baishideng Publishing Group co., Limited. All rights reserved.Key words: Mass spectrometry; Lipidomics; Phospholipids; Serial signaling; Neuroscience Core tip: Mass spectrometry offers a degree of simplicity and sophistication to the biological sciences. In this review we are focusing on its application towards the analysis of lipids in neuroscience. Lipids have a variety of functions, they surround neurons, provide insulation for transmission of signals, an environment for facilitating motility and migration of astrocytes and other cell types, among many other functions. Recent advances in mass spectrometry have enabled quantification of lipids directly extracted from complex biological mixtures in the neuronal system with the help of databases, standardized instrument parameters and bioinformatics. In this review, we intend to highlight all recent efforts with an emphasis on its application to neuroscience.Enriquez-Algeciras M, Bhattacharya SK. Lipidomic mass spectrometry and its application in neuroscience. World J Biol Chem 2013; 4(4): 102-110 Available from:

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Toward fingerprinting cellular lipidomes directly from biological samples by two-dimensional electrospray ionization mass spectrometry

Cited by 212 publications

References 34 publications

Lipidomics for Elucidation of Metabolic Syndrome and Related Lipid Metabolic Disorder

Lipidomics for Elucidation of Metabolic Syndrome and Related Lipid Metabolic Disorder

Lipidomic platform for structural identification of skin ceramides with α-hydroxyacyl chains

Lipidomic mass spectrometry and its application in neuroscience

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