Lipidomic mass spectrometry and its application in neuroscience

Enriquez-Algeciras, Mabel; Bhattacharya, Sanjoy K.

doi:10.4331/wjbc.v4.i4.102

Cited by 10 publications

(7 citation statements)

References 61 publications

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“…The analyses were performed using LipidSearch 4.1 (Thermo Fisher Scientific) and MZmine 2.9 with LIPID MAPS derived database. Quantification utilized class‐specific standards in a 2‐step process developed for automated lipid quantification . These concentrations were normalized to total protein content.…”

Section: Methodsmentioning

confidence: 99%

Endogenous ocular lipids as potential modulators of intraocular pressure

Edwards

Arcuri

Wang

et al. 2020

J Cellular Molecular Medi

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Elevated intraocular pressure (IOP) is a risk factor in glaucoma, a group of irreversible blinding diseases. Endogenous lipids may be involved in regulation of IOP homeostasis. We present comparative fold analysis of phospholipids and sphingolipids of aqueous humour and trabecular meshwork from human control vs primary openangle glaucoma and mouse control (normotensive) vs ocular hypertensive state. The fold analysis in control vs disease state was based on ratiometric mass spectrometric data for above classes of lipids. We standardized in vitro assays for rapid characterization of lipids undergoing significant diminishment in disease state. Evaluation of lipids using in vitro assays helped select a finite number of lipids that may potentially expand cellular interstitial space embedded in an artificial matrix or increase fluid flow across a layer of cells. These assays reduced a number of lipids for initial evaluation using a mouse model, DBA/2J with spontaneous IOP elevation. These lipids were then used in other mouse models for confirmation of IOP lowering potential of a few lipids that were found promising in previous assessments. Our results provide selected lipid molecules that can be pursued for further evaluation and studies that may provide insight into their function. K E Y W O R D Saqueous humour, glaucoma, lipidomics, lipids, trabecular meshwork | 3857 EDWARDS Et Al.

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Section: Methodsmentioning

confidence: 99%

Endogenous ocular lipids as potential modulators of intraocular pressure

Edwards

Arcuri

Wang

et al. 2020

J Cellular Molecular Medi

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“…54 There is also research on neurotrophic factor intraocular implants, 55 as well as efforts towards discovering lipid entities capable of neuronal regeneration. 56…”

Section: Ophthalmologymentioning

confidence: 99%

A Multidisciplinary Consensus for Clinical Care and Research Needs for Sturge-Weber Syndrome

Torre

Luat

Juhász

et al. 2018

Pediatric Neurology

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“…Auxiliary gas (Argon) was set to five arbitrary units. Settings for analyses of different phospholipid classes were established based on previous studies [Enriquez‐Algeciras and Bhattacharya, ]. The 0.1% formic acid (FA) was used as an additive method for analyses of lipids in the positive ion mode only (i.e.,, for analyses of PC but not for PS).…”

Section: Methodsmentioning

confidence: 99%

Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy

Suárez

Piqueras

Correa

et al. 2017

J of Cellular Biochemistry

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Climatic droplet keratopathy (CDK) is an acquired degenerative disease predominantly affecting males over 40 years old. It results in progressive corneal opacities usually affecting both eyes. CDK is multifactorial and its etiology remains unknown. Our recent findings are consistent with CDK pathology being driven by environmental factors with oxidative stress playing an important role (for example, contributing to lipid peroxidation) rather than climate factors. The changes in corneal lipid composition affected by environmental factors remain understudied. The purpose of this study was to systematically investigate phospholipids profile [phosphatidylcholine (PC) and phosphatidylserine (PS)] in corneas from CDK patients using tandem mass spectrometry. Samples from CDK areas and from non-affected areas were obtained from patients diagnosed with CDK who underwent cataract surgery, were subjected to lipid extraction using a modified Bligh and Dyer method; protein concentrations were determined using the Bradford’s method. Lipids were identified and subjected to ratiometric quantification using TSQ Quantum Access Max triple quadrupole mass spectrometer, using appropriate class specific lipid standards. All phospholipid classes showed lower total amounts in affected areas compared to control areas from CDK’s corneas. Comparative profiles of two phospholipid classes (PC, PS) between CDK areas and control areas showed several common species between them. We also found a few unique lipids that were absent in CDK areas compared to controls and vice versa. Lower amount of phospholipids in CDK areas compared to control areas could be attributed to the lipid peroxidation in the affected corneal regions as a consequence of increased oxidative stress.

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Lipidomic mass spectrometry and its application in neuroscience

Cited by 10 publications

References 61 publications

Endogenous ocular lipids as potential modulators of intraocular pressure

Endogenous ocular lipids as potential modulators of intraocular pressure

A Multidisciplinary Consensus for Clinical Care and Research Needs for Sturge-Weber Syndrome

Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy

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