Total internal reflection–based module for fluorescence and absorbance detection

Verschooten, Tom; Ottevaere, Heidi; Vervaeke, Michael; Erps, Jürgen Van; Thienpont, Hugo

doi:10.1117/1.jmm.13.3.033001

Cited by 7 publications

(23 citation statements)

References 11 publications

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“…Misalignment of the chip should hence not deviate the excitation beam. (iii) The system presented in our previous work [11,12] was highly sensitive to presence of air bubbles. In case one or more air bubbles were present, the measurement had to be repeated.…”

Section: Optofluidic Chip Designmentioning

confidence: 97%

Optofluidic multi-measurement system for the online monitoring of lubricant oil

Verschooten

Callewaert

Ciaccheri

et al. 2015

Meas. Sci. Technol.

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We show a detection system that simultaneously allows absorbance (ABS), laser-induced fluorescence (LIF) and scattering detection excited by two different laser sources at 405 nm and 450 nm. The heart of the system consists of a mass manufacturable polymer optofluidic chip. The chip is mounted in an optical detection assembly that aligns the chip to the rest of the system, seals the chip from leakage, fixes the position and connects the channels to the rest of the fluidic system. The fluidics exhibit a reduced susceptibility to perturbations caused by air bubbles, this is accomplished by making use of a serpentine channel layout. For coumarin 480, detection limits of 100 nM and 10 pM are observed for ABS and LIF respectively. An effective detection range of 4000 down to 1 nephelometric turbidity units is shown for the detection of scattered light. The viscous behaviour of the sample is analysed by a secondary FFT processing step of which the result is further processed by multivariate data analysis. This allows the identification of samples and prediction of their quality parameters. We apply this system for the monitoring of lubricant oil, demonstrating its ability to compete with spectroscopic detection techniques. The low-cost approach and multi-measurement architecture shown in this paper pave the way for miniaturized on-line monitoring of liquids in an industrial environment.

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Section: Optofluidic Chip Designmentioning

confidence: 97%

Optofluidic multi-measurement system for the online monitoring of lubricant oil

Verschooten

Callewaert

Ciaccheri

et al. 2015

Meas. Sci. Technol.

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“…Because the reaction principle is based on enzyme inhibition, temperature should be considered . For the process of reflection absorbance detection, the more appropriate the wavelength of the detected light the better . When the liquid solution flows inside the chip using surface tension, the influence of gravity should not be ignored .…”

Section: Microfluidic Detection Device Driven By Liquid Surface Tensionmentioning

confidence: 99%

“…According to the color of the reaction product in the pesticide test, the illumination detector should be determined to optimize the reflection absorption effect. Only the appropriate wavelength range can truly capture the proportional relationship between the pesticide concentration and the light absorption . Therefore, the test was carried out on a paper‐based microfluidic chip using pesticide sample reagents at concentrations of 0.1, 0.15, 0.25, 0.3, and 0.4 mg/L.…”

Section: Microfluidic Detection Device Driven By Liquid Surface Tensionmentioning

confidence: 99%

“…Obviously, the temperature and the wavelength of the detected light still have a significant influence on the detection accuracy of the microfluidic chip platform driven by the micromotor , and the effect is similar to the microfluidic chip driven by surface tension. While the former chip has some other special influencing factors that need our attention.…”

Section: Microfluidic Detection Device Driven By Liquid Surface Tensionmentioning

confidence: 99%

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A review on microfluidics in the detection of food pesticide residues

Jie

et al. 2019

Electrophoresis

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This paper briefly explains the food safety problems related to pesticide residues and introduces microfluidics technology as a pesticide residue detection method. Three mainstream microfluidic detection devices are detailed: one driven by liquid surface tension, one by motor siphon drive, and one by centrifugal force. The advantages and disadvantages of each are considered in an analysis of future trends in microfluidic technology for pesticide detection.

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“…This mask can stop protons with an energy of up to 16.5 MeV, 14 enabling irradiation of PMMA substrates that are up to 2 mm thick. 15 The concept of DPW is based on the fact that irradiating high-molecular-weight PMMA with highly energetic protons will break the long polymer chains. This can be exploited because it changes the physical and chemical material properties of the unexposed bulk material, allowing these irradiated zones to be selectively etched away.…”

Section: Prototyping By Deep Proton Writingmentioning

confidence: 99%

Replication of self-centering optical fiber alignment structures using hot embossing

et al. 2016

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With the demand for broadband connectivity on the rise due to various services like video-on-demand and cloud computing becoming more popular, the need for better connectivity infrastructure is high. The only futureproof option to supply this infrastructure is to deploy "fiber to the home" (FTTH) networks. One of the main difficulties with the deployment of FTTH is the vast amount of single-mode fiber (SMF) connections that need to be made. Hence there is a strong need for components which enable high performance, robust and easy-touse SMF connectors. Since large-scale deployment is the goal, these components should be mass-producible at low cost. We discuss a rapid prototyping process on the basis of hot embossing replication of a self-centering alignment system (SCAS) based on three micro-springs, which can position a SMF independently of its diameter. This is beneficial since there is a fabrication tolerance of up to ±1 µm on a standard G.652 SMF's diameter that can lead to losses if the outer diameter is used as a reference for alignment. The SCAS is first prototyped with deep proton writing (DPW) in polymethylmethacrylate (PMMA) after which it is glued to a copper substrate with an adhesive. Using an electroforming process, a nickel block is grown over the PMMA prototype followed by mechanical finishing to fabricate a structured nickel mould insert. Even though the mould insert shows nonideal and rounded features it is used to create PMMA replicas of the SCAS by means of hot embossing. The SCAS possesses a central opening in which a bare SMF can be clamped, which is designed with a diameter of 121 µm. PMMA replicas are dimensionally characterized using a multisensor coordinate measurement machine and show a central opening diameter of 128.3 ± 2.8 µm. This should be compared to the central opening diameter of the DPW prototype used for mould formation which was measured to be 120.5 µm. This shows that the electroforming and subsequent replication process is possible for complex micro-scale components and could be accurate after optimisation. We characterized the sidewall roughness of PMMA replicas using a noncontact optical profiler, resulting in a root-mean-square roughness of 48 nm over an area of 63.7 µm×47.8 µm. This low sidewall roughness is especially important in the replication of high aspect ratio structures to facilitate demoulding since the sidewalls cause the most friction with the mould insert.

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Total internal reflection–based module for fluorescence and absorbance detection

Cited by 7 publications

References 11 publications

Optofluidic multi-measurement system for the online monitoring of lubricant oil

Optofluidic multi-measurement system for the online monitoring of lubricant oil

A review on microfluidics in the detection of food pesticide residues

Replication of self-centering optical fiber alignment structures using hot embossing

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