Total creatine in muscle: imaging and quantification with proton MR spectroscopy.

Bottomley, Paul A.; Lee, Yung Hsiang; Weiss, Robert G.

doi:10.1148/radiology.204.2.9240527

Cited by 74 publications

(75 citation statements)

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“…The concentration of creatine is rather stable in muscular tissue (approximately 30 mmol/kg wet weight) and shows little inter-individual and regional variations (97)(98)(99), despite the fact that creatine concentration changes slightly with nutrition (100,101). In addition, creatine signals did not vary before and for sufficiently long period after exercise in an MR study (65).…”

Section: Calibration By the Creatine Signalmentioning

confidence: 99%

Role of proton MR for the study of muscle lipid metabolism

et al. 2006

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1 H-MR spectroscopy (MRS) of intramyocellular lipids (IMCL) became particularly important when it was recognized that IMCL levels are related to insulin sensitivity. While this relation is rather complex and depends on the training status of the subjects, various other influences such as exercise and diet also influence IMCL concentrations. This may open insight into many metabolic interactions; however, it also requires careful planning of studies in order to control all these confounding influences. This review summarizes various historical, methodological, and practical aspects of 1 H-MR spectroscopy (MRS) of muscular lipids. That includes a differentiation of bulk magnetic susceptibility effects and residual dipolar coupling that can both be observed in MRS of skeletal muscle, yet affecting different metabolites in a specific way. Fitting of the intra-(IMCL) and extramyocellular (EMCL) signals with complex line shapes and the transformation into absolute concentrations is discussed. Since the determination of IMCL in muscle groups with oblique fiber orientation or in obese subjects is still difficult, potential improvement with high-resolution spectroscopic imaging or at higher field strength is considered. Fat selective imaging is presented as a possible alternative to MRS and the potential of multinuclear MRS is discussed. 1 H-MRS of muscle lipids allows non-invasive and repeated studies of muscle metabolism that lead to highly relevant findings in clinics and patho-physiology.

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Section: Calibration By the Creatine Signalmentioning

confidence: 99%

Role of proton MR for the study of muscle lipid metabolism

et al. 2006

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“…In human lower leg muscle the amount of tCr is 36.2 mmol/kg wet weight by MRS, which is consistent with biopsy analysis. 81 It should be emphasized, however, that Cr/PCr is anisotropically oriented to muscle fibers in human skeletal muscle as concluded from incomplete averaging of dipolar coupling. 56 The lack of motional narrowing confirms restricted mobility of Cr.…”

Section: In Vivo Applicationsmentioning

confidence: 99%

Magnetization transfer MRS

Leibfritz¹,

Dreher²

2001

NMR in Biomedicine

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This review deals with magnetization transfer (MT) effects observed in in vivo NMR spectroscopy. The basic experimental methods of MT experiments, the underlying kinetic mechanisms as well as the evaluation of measured data by fits to two-or three-pool models are described. Experimental results of both 31 P and 1 H in vivo MRS are reviewed showing the potential of MT experiments to characterize kinetic equilibrium reactions. This includes reactions where all involved components are MR visible, as well as situations where one indirectly measures pools of bound spins which cannot directly be observed in vivo. In particular, MT effects are described which have been observed in in vivo 1 H NMR spectra measured on the animal or human brain or on skeletal muscle. Possible mechanisms for the strong MT effects observed for the signals of creatine/phosphocreatine, lactate, alcohol and other metabolites are discussed. It is also emphasized that MT effects caused by water suppression techniques may lead to systematic errors in the quantification of in vivo 1 H NMR spectra.

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“…The still very low signal intensities of creatine might lead to further fitting errors and therefore affect the final quantification precision. Signal amplitudes of the ERETIC reference can be freely chosen, and additionally, the ERETIC reference can be applied to all conditions including pathological cases, whereas the concentration of creatine may change in muscle disorders and literature values for muscular creatine content in healthy subjects also vary between 27.2 and 36.2 mmol/kg ww (25). In comparison to the external reference standard, the ERETIC method has proven to be more reliable ( Table 1).…”

Section: Discussionmentioning

confidence: 99%

Optically transmitted and inductively coupled electric reference to access in vivo concentrations for quantitative proton‐decoupled ¹³C magnetic resonance spectroscopy

Chen

Pavan

Heinzer-Schweizer

et al. 2011

Magnetic Resonance in Med

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This report describes our efforts on quantification of tissue metabolite concentrations in mM by nuclear Overhauser enhanced and proton decoupled 13 C magnetic resonance spectroscopy and the Electric Reference To access In vivo Concentrations (ERETIC) method. Previous work showed that a calibrated synthetic magnetic resonance spectroscopy-like signal transmitted through an optical fiber and inductively coupled into a transmit/receive coil represents a reliable reference standard for in vivo 1 H magnetic resonance spectroscopy quantification on a clinical platform. In this work, we introduce a related implementation that enables simultaneous proton decoupling and ERETIC-based metabolite quantification and hence extends the applicability of the ERETIC method to nuclear Overhauser enhanced and proton decoupled in vivo 13 C magnetic resonance spectroscopy. In addition, ERETIC signal stability under the influence of simultaneous proton decoupling is investigated. The proposed quantification method was cross-validated against internal and external reference standards on human skeletal muscle. The ERETIC signal intensity stability was 100.65 6 4.18% over 3 months including measurements with and without proton decoupling. Glycogen and unsaturated fatty acid concentrations measured with the ERETIC method were in excellent agreement with internal creatine and external phantom reference methods, showing a difference of 1.85 6 1.21% for glycogen and 1.84 6 1.00% for unsaturated fatty acid between ERETIC and creatinebased quantification, whereas the deviations between external reference and creatine-based quantification are 6.95 6 9.52% and 3.19 6 2.60%, respectively. Magn Reson Med 67:1-7, 2012. V C 2011 Wiley Periodicals, Inc. Key words:13 C magnetic resonance spectroscopy; glycogen quantification; inductive coupling; electric reference to access in vivo concentrations; unsaturated fatty acid concentration Millimolar (mM) concentrations of metabolites in specific tissues are of fundamental interest to the understanding of metabolism in clinical diagnostics and physiological studies. Magnetic resonance spectroscopy (MRS) is a powerful technique for this purpose because of the direct proportionality of signal intensities in spectra and the amount of resonating nuclei (1,2). Although the internal water reference method has become standard for the quantification of metabolite concentrations in mM (absolute quantification) by 1 H MRS (3), absolute quantification by heteronuclear MRS is a particularly difficult but important task. The goal of this study was to establish a reliable and stable method that enables absolute quantification of metabolite concentrations by in vivo 13 C MRS in physiological and pathological conditions. Unlike in 1 H MRS, internal reference standards are not easily available for in vivo 13 C MRS. The internal water reference method is unreliable, because the transmit and receive B 1 field patterns in the related proton measurement differ from the B 1 fields in the heteronuclear measurement. This problem ...

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Total creatine in muscle: imaging and quantification with proton MR spectroscopy.

Abstract: H-1 MR spectroscopy can be used to image and noninvasively quantify total creatine in human muscle. Its use could improve the understanding of the role of altered creatine metabolism in muscle disease and aid quantification of the response to creatine therapies.

Cited by 74 publications

References 0 publications

Role of proton MR for the study of muscle lipid metabolism

Role of proton MR for the study of muscle lipid metabolism

Magnetization transfer MRS

Optically transmitted and inductively coupled electric reference to access in vivo concentrations for quantitative proton‐decoupled ¹³C magnetic resonance spectroscopy

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Total creatine in muscle: imaging and quantification with proton MR spectroscopy.

Abstract: H-1 MR spectroscopy can be used to image and noninvasively quantify total creatine in human muscle. Its use could improve the understanding of the role of altered creatine metabolism in muscle disease and aid quantification of the response to creatine therapies.

Cited by 74 publications

References 0 publications

Role of proton MR for the study of muscle lipid metabolism

Role of proton MR for the study of muscle lipid metabolism

Magnetization transfer MRS

Optically transmitted and inductively coupled electric reference to access in vivo concentrations for quantitative proton‐decoupled 13C magnetic resonance spectroscopy

Contact Info

Product

Resources

About

Optically transmitted and inductively coupled electric reference to access in vivo concentrations for quantitative proton‐decoupled ¹³C magnetic resonance spectroscopy