Toscana Virus in Spain

Sanbonmatsu‐Gámez, Sara; Pérez-Ruiz, Mercedes; Collao, Ximena; Sánchez-Seco, María Paz; Márquez, Fernando José; Rosa-Fraile, Manuel; Navarro-Marí, José María; Tenorio, Antonio

doi:10.3201/eid1111.050851

Cited by 95 publications

(104 citation statements)

References 28 publications

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“…A collection of 248 human sera previously described in Sanbonmatsu-Gamez and others 14 was assayed. The serum collection corresponds to healthy individuals from the metropolitan area of Granada collected in 2003.…”

Section: Methodsmentioning

confidence: 99%

Granada Virus: a Natural Phlebovirus Reassortant of the Sandfly Fever Naples Serocomplex with Low Seroprevalence in Humans

Collao

Palacios²,

Ory³

et al. 2010

The American Society of Tropical Medicine and Hygiene

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Abstract.A new member of the phlebovirus genus, tentatively named Granada virus, was detected in sandflies collected in Spain. By showing the presence of specific neutralizing antibodies in human serum collected in Granada, we show that Granada virus infects humans. The analysis of the complete genome of Granada virus revealed that this agent is likely to be a natural reassortant of the recently described Massilia virus (donor of the long and short segments) with a yet unidentified phlebovirus (donor of the medium segment).

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Section: Methodsmentioning

confidence: 99%

Granada Virus: a Natural Phlebovirus Reassortant of the Sandfly Fever Naples Serocomplex with Low Seroprevalence in Humans

Collao

Palacios²,

Ory³

et al. 2010

The American Society of Tropical Medicine and Hygiene

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“…In endemic regions, such as countries in the Mediterranean basin, exposure to SFVs is common as revealed by serosurveys, and TOSV infections account for a high proportion of human aseptic meningitis (Eitrem et al 1991a, Nicoletti et al 1996, Hemmersbach-Miller et al 2004, Sanbonmatsu-Gamez et al 2005.…”

mentioning

confidence: 99%

“…In acute cases, methods for the diagnosis of TOSV infections include direct detection of the virus in patient samples either by culture or by nucleic acid amplification techniques and virus serology (Dionisio et al 2003, Hemmersbach-Miller et al 2004, Sanbonmatsu-Gamez et al 2005. Investigation of TOSV-specific immunoglobulins is also important for identifying virus exposure in healthy subjects from endemic countries or in regions where the sandfly vectors are present (Dionisio et al 2003).…”

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confidence: 99%

Performance of Various Commercial Assays for the Detection of Toscana Virus Antibodies

Ergünay

Litzba

Lô

et al. 2011

Vector-Borne and Zoonotic Diseases

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Introduction: Sandfly fever virus (SFV) serotypes sandfly fever Naples virus, sandfly fever Sicilian virus, and sandfly fever Cyprus virus cause febrile diseases, whereas Toscana virus (TOSV) is responsible for aseptic meningoencephalitis. Diagnosis and surveillance of TOSV depend heavily on virus serology, and various commercial assays utilizing various antigen sources and formats have been available. The aim of this study was to perform comparative evaluation of commercially available serological assays for anti-TOSV immunoglobulins. Materials and Methods: A collection of 120 sera from healthy blood donors from an endemic region, previously identified to be reactive for antibodies against various SFV serotypes by indirect immunofluorescence test (IIFT), was reevaluated for IgG/IgM via IIFT, enzyme-linked immunosorbent assay, and an immunoblot assay manufactured by Euroimmun, Diesse, and Mikrogen, respectively. Virus neutralization test (VNT) was performed for 99 sera using standard TOSV, sandfly fever Sicilian virus, and sandfly fever Naples virus strains. Results: A total of 89 samples (74.2%) were reactive for TOSV IgG in at least one of the commercial assays, and 31 samples (31.3%) were reactive in VNT for various SFV serotypes. Average percentage agreements among commercial assays and between VNT and the commercial assays were noted as 57.8% and 62.6%, respectively. No significant correlation between assay results and VNT titers was observed. SFV IgM antibodies were detected in a total of eight samples (6.7%) via IIFT, which were nonreactive in enzyme-linked immunosorbent assay and VNT. Discussion: Commercial diagnostic immunoassays displayed slight to fair agreement for TOSV IgG as assessed via kappa and percentage agreement values. The results could only be confirmed via virus neutralization in a portion of the samples, and overall agreement between the commercial assays and VNT was slight. Commercial assays such as immunoblot can be used in addition to VNT for confirmation of TOSV exposure.

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“…Later the virus was also isolated from the sand fly P. perfiliewi (Valassina et al 2003). However, P. perniciosus is probably the most common insect vector of the Toscana virus, being one of the most abundant sand flies in southern Europe (Maroli et al 1994;Sanbonmatsu-Gámez et al 2005). With tourists, returning from Mediterranean countries, the virus has been imported to further European countries such as Germany, Sweden and Switzerland (Cusi et al 2010).…”

Section: Bunyaviridaementioning

confidence: 99%