Granada Virus: a Natural Phlebovirus Reassortant of the Sandfly Fever Naples Serocomplex with Low Seroprevalence in Humans

Collao, Ximena; Palacios, Gustavo; Ory, Fernando de; Sanbonmatsu, Sara; Pérez-Ruiz, Mercedes; Navarro, José María; Molina, Ricardo; Hutchison, Stephen; Lipkin, W. Ian; Tenorio, Antonio; Sánchez-Seco, María Paz

doi:10.4269/ajtmh.2010.09-0697

Cited by 69 publications

(63 citation statements)

References 32 publications

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“…9 Although neutralization tests are considered more specific than IFA, negative results by the former do not exclude infection. Thus, positive results by IFA should not be always considered false positives when neutralization assays are negative.…”

Section: Discussionmentioning

confidence: 99%

“…9 Neurological processes were not included, because no phlebovirus other than TOSV was detected in any of 457 CSF samples received in our laboratory during the last 4 years. In the regional reference laboratory for diagnosis of viral meningitis and encephalitis (Andalusia, Spain), phleboviruses' investigation was routinely carried out in CSF samples by using a generic reversetranscription-nested polymerase chain reaction (PCR) 16 (data not shown).…”

Section: Discussionmentioning

confidence: 99%

“…The presence of this virus in phlebotomines has been reported later in other areas of Spain (Balearic Islands and Catalonia). 9 Phylogenetic analysis of the complete genome has confirmed that GRV belongs to SFNV serocomplex and is closely related to Massilia virus, another new phlebovirus described in southern France. 10 GRV infection rate in phlebotomines is approximately 0.2%, much higher than the rate of 0.05% reported for TOSV.…”

Section: Introductionmentioning

confidence: 97%

“…10 GRV infection rate in phlebotomines is approximately 0.2%, much higher than the rate of 0.05% reported for TOSV. 2,9 Whether GRV causes infections in humans is still unknown. This work focused on two main objectives.…”

Section: Introductionmentioning

confidence: 99%

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Clinic–Epidemiologic Study of Human Infection by Granada Virus, a New Phlebovirus within the Sandfly Fever Naples Serocomplex

Navarro-Marí

Gómez-Camarasa²,

Pérez-Ruiz³

et al. 2013

The American Journal of Tropical Medicine and Hygiene

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Abstract. Granada virus (GRV), a new phlebovirus within the Naples serocomplex, has been recently described in phlebotomine sandflies from Spain. The presence of anti-GRV immunoglobulin G (IgG) antibodies was investigated by indirect fluorescence assay (IFA) and neutralization test (NT) in 920 serum samples from the Granada population. By IFA, an overall GRV seroprevalence of 15.8% (N = 145) was observed, significantly increasing up to 65 years. NT was positive in 18% of anti-GRV IFA-positive samples. IgG antibodies against Toscana virus (TOSV), a hyperendemic phlebovirus within Granada province, were detected in 40% of anti-GRV-positive cases. Anti-GRV IgM antibodies were detected in 36 (6.6%) of 547 acute-phase serum samples from individuals with febrile illness, exanthema, and/or acute respiratory infection. All positives were anti-TOSV IgM-negative. GRV may infect humans, with most cases being asymptomatic. The codetection of anti-GRV and anti-TOSV IgG antibodies could be attributable to cross-reactivity or exposure to the same transmission vector.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Clinic–Epidemiologic Study of Human Infection by Granada Virus, a New Phlebovirus within the Sandfly Fever Naples Serocomplex

Navarro-Marí

Gómez-Camarasa²,

Pérez-Ruiz³

et al. 2013

The American Journal of Tropical Medicine and Hygiene

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“…21,22 In areas without circulation of other known phleboviruses (e.g., Toscana, Naples, Sicilian, Arumowot, and Punta Toro viruses), [23][24][25] the HAI has shown high sensitivity and specificity (compared with plaque reduction neutralization testing) for detection of RVFV infection in animals, with performance equal to that of an IgG enzyme-linked immunosorbent assay. 22 At the time of the study reported here, the HAI was the only assay used at the collaborating South African veterinary laboratory.…”

Section: Study Populationmentioning

confidence: 99%

Rift Valley Fever Virus Infection in African Buffalo (Syncerus caffer) Herds in Rural South Africa: Evidence of Interepidemic Transmission

LaBeaud¹,

Cross

Getz

et al. 2011

The American Journal of Tropical Medicine and Hygiene

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Abstract. Rift Valley fever virus (RVFV) is an emerging biodefense pathogen that poses significant threats to human and livestock health. To date, the interepidemic reservoirs of RVFV are not well defined. In a longitudinal survey of infectious diseases among African buffalo during [2000][2001][2002][2003][2004][2005][2006], 550 buffalo were tested for antibodies against RVFV in 820 capture events in 302 georeferenced locations in Kruger National Park, South Africa. Overall, 115 buffalo (21%) were seropositive. Seroprevalence of RVFV was highest (32%) in the first study year, and decreased progressively in subsequent years, but had no detectable impact on survival. Nine (7%) of 126 resampled, initially seronegative animals seroconverted during periods outside any reported regional RVFV outbreaks. Seroconversions for RVFV were detected in significant temporal clusters during 2001-2003 and in 2004. These findings highlight the potential importance of wildlife as reservoirs for RVFV and interepidemic RVFV transmission in perpetuating regional RVFV transmission risk. Sabie (2003Sabie ( -2006 regions of the park ( Figure 1 ). Briefly, from a defined sub-region of Kruger National Park having a buffalo population of approximately 3,000, 593 buffalo were captured at 302 georeferenced locations by using aerial or ground darting techniques, working in close collaboration with veterinarians in Kruger National Park.Captured animals were tested for exposure to infections and uniquely branded and given subcutaneous insertion of microchip tags for later identification. A subset (n = 167) were fitted with either radio collars or global positioning system collars for tracking and weekly to monthly follow-up. Because male and female buffalo have horns, it was impossible for a radio collar to come off an animal without tearing through the belting. We identified mortality events by the belting still being intact and the remains of a carcass. We assumed that a radio collar had fallen off if the belting was severed and no carcass was present after an extensive search of the area. 18 We estimated time since death (0-30 days) using the extent of carcass remains.18 During the study, there were 288 live repeat captures among the 593 buffalo studied and 55 documented deaths among the 167 tracked animals.Ethical approval. Institutional Animal Care and Use Committee approval was through University of California Berkeley #R217-0402 to Wayne M. Getz. This protocol was also reviewed and approved by the Animal Care and Use Committee for the South Africa National Parks.Animal assessment. Animals < 5 years of age were assigned to an age group on the basis of physical examination for incisor eruption pattern.19 Age of animals ≥ 5 years of age at the time of first examination was determined by visual comparison of horn development and body size to a calibrated photographic reference collection. 17,20 Four age groups were considered: youngest = 1-3 years, mid-low = 3-5 years, mid-high = 5-8 years, highest = more than 8 years.Testing for antibodies ...

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Sandmückenfieber – eine „vernachlässigte“ Krankheit

et al. 2018

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A 45-year-old woman presented at the outpatient department of a center for tropical diseases with fever, diarrhea, headache, myalgia, malaise, and an itchy papular rash. She had been on holiday with her family for 11 days in a mountain village in northern Cyprus. The place was infested with a lot of small, stinging flies or mosquitoes. She and her family became sick after they returned home. The physical examination was normal apart from the rash on the inside of the extremities. Significantly elevated transaminases and a slightly increased C‑reactive protein level were found in the blood examination. Considering the country of travel, the report of the "stinging flies" and the clinical presentation, sandfly fever was also taken into account as a differential diagnosis for the hepatitis. Antibodies to the sandfly fever Sicilian virus (SFSV) were detected. They showed the typical dynamics during the course of the illness and thus "pappataci fever" was diagnosed. The case report and a short review of up-to-date literature is meant encourage consideration of phlebovirus infection as a possible differential diagnosis in travelers or refugees suffering from severe febrile hepatitis and rash or aseptic viral meningitis after their stay in the Mediterranean area.

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Granada Virus: a Natural Phlebovirus Reassortant of the Sandfly Fever Naples Serocomplex with Low Seroprevalence in Humans

Cited by 69 publications

References 32 publications

Clinic–Epidemiologic Study of Human Infection by Granada Virus, a New Phlebovirus within the Sandfly Fever Naples Serocomplex

Clinic–Epidemiologic Study of Human Infection by Granada Virus, a New Phlebovirus within the Sandfly Fever Naples Serocomplex

Rift Valley Fever Virus Infection in African Buffalo (Syncerus caffer) Herds in Rural South Africa: Evidence of Interepidemic Transmission

Sandmückenfieber – eine „vernachlässigte“ Krankheit

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