Topical treatment of standardised burns with herbal remedies in model rats

Abstract

Experimental studies of burns require the use of different animal models. The aim of this work was to establish experimental model of thermal injuries and to evaluate the effects of topical agents on healing of the burn wounds. Forty female Wistar rats were randomly classified in 4 groups and isolated for 2 weeks before the onset of experiment. Animals were primarily anaesthetized with pentobarbital-sodium and then shaved (skin area of their back with diameters 5 cm x 5 cm). A round metal stamp with contact area of 5 cm2 and total weight of 100 g was heated up to 80 degrees C and then applied without additional pressure on the depilated skin of the back for 14 seconds. This procedure produced a standardized burn wound. Induced burn wounds were immediately drowned in the 4 degrees C- water for 3 s in order to maintain microcirculation. After the inducement of thermal injures, all rats were treated with 1% silver sulfadiazine cream, herbal topical preparations or were not treated at all. Burn wounds were treated twice a day until the healing completion. The result of treatment application was a significant reduction of burn wound diameters. Herbal topical preparations expressed positive therapeutic effects on the parameters of burn wounds. The efficiency of silver sulfadiazine cream in burn wound healing was significantly more expressed in comparison to healing process in control group of animals (p < or = 0,001). We conclude that herbal topical preparations efficiently caused the completion of burn wound healing process without scar formation.

Catalytic Room‐Temperature C−N Coupling of Amides and Isocyanates by Using Mechanochemistry

“…Despite recent progress and calls of pharmaceutical industries for development of cleaner and more efficient reactivity, most methods of amide synthesis or functionalization are tethered to traditional solution protocols. Consequently, we recognized the application of mechanochemical C−N coupling to amide substrates would be an advance in the green chemistry of amides and offer a new one‐step and room‐temperature route to carbamoyl amide moieties relevant in insecticides (Flucycloxuron, Triflumuron) and pharmaceuticals (Cefoperazone, Cabergoline, Glimepiride) …”

Sustained drug delivery to reduce the extent of burn progression

“…Full thickness burn models are ideal for testing of full thickness scaffolds (Ramakrishnan et al, 2022). For preliminary testing of topical agents, it is best to use partial or deep partial thickness burn models (Becic et al, 2005). The findings of 55°C burn condition reveals that the initial partial thickness burn has progressed in to a deep partial thickness burn.…”