Topical formulation containing Ilex Paraguariensis extract increases metalloproteinases and myeloperoxidase activities in mice exposed to UVB radiation

Cuelho, Camila Helena Ferreira; Alves, Geórgia de Assis Dias; Lovatto, Marina Ortiz; Bonilha, Iuri França; Barbisan, Fernanda; Cruz, Ivana Beatrice Mânica da; Oliveira, Sara Marchesan; Fachinetto, Roselei; Canto, Gizele Scotti do; Manfron, Melânia Palermo

doi:10.1016/j.jphotobiol.2018.10.004

Cited by 18 publications

(14 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Use of a chemotherapeutic that works by a different mechanism, with 4,5-diCQA, should produce synergistic inhibitory effect on prostate cancer. It is also worth noting that studies so far reported in literature with YM extracts demonstrated no inhibitory activity on normal cells such as noncancerous CCD-18Co cell line [7] and fibroblasts [10]. The results from our studies with NIH-3T3 and MC-3T3 cells further confirmed the lack of toxicity on normal cells which would provide a higher therapeutic index for 4,5-diCQA on prostate cancer.…”

Section: Discussionsupporting

confidence: 80%

See 1 more Smart Citation

Inhibition of Prostate Cancer Cells by 4,5-Dicaffeoylquinic Acid through Cell Cycle Arrest

et al. 2019

View full text Add to dashboard Cite

Prostate cancer is a major cause of cancer-related mortality in men. Even though current therapeutic management has contributed to reducing mortality, additional intervention strategies are warranted to further improve the outcomes. To this end, we have investigated the efficacy of dicaffeoylquinic acids, ingredients in Yerba Mate (Ilex paraguariensis), an evergreen cultivated in South America, the leaves of which are used to prepare a tea/coffee-like drink. Of the various analogs tested, 4,5-dicaffeoylquinic acid (4,5-diCQA) was the most active molecule against DU-145 prostate cancer cells with a 50% inhibitory concentration (IC50) of 5 μM. 4,5-diCQA was active both under normoxic and hypoxic conditions. The effect of 72-hour treatment on DU-145 cells persisted for an extended time period as assessed by clonogenic assay. Mechanistic studies revealed that the toxic effect was not due to induction of programmed cell death but through cell cycle arrest at S phase. Additionally, 4,5-diCQA did not impact PI3K/MAPK signaling pathway nor did it affect the depolarization of the mitochondrial membrane. 4,5-diCQA-induced accumulation of cells in the S-phase also seems to negatively impact Bcl-2 expression. 4,5-diCQA also exhibited inhibitory activity on LNCaP and PC-3 prostate cancer cells suggesting that it has therapeutic potential on a broad range of prostate cancers. Taken together, the novel inhibitory activity and mechanism of action of 4,5-diCQA opens up potential therapeutic options for using this molecule as monotherapy as well as in combinatorial therapies for the clinical management of prostate cancer.

show abstract

Section: Discussionsupporting

confidence: 80%

“…Multiple studies have focused on the biological activity of the YM extracts. Cuelho et al, in mice studies, observed chemoprotective effect of YM extract containing polyphenols on UVB exposure [10]. YM consumption also lowered the total blood cholesterol in subjects with dyslipidemia [11].…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Prostate Cancer Cells by 4,5-Dicaffeoylquinic Acid through Cell Cycle Arrest

et al. 2019

View full text Add to dashboard Cite

show abstract

“…In addition, the lipid peroxidation process leads to the expression of phospholipases and cyclooxygenase with subsequent production of prostaglandins, which are responsible for epithelial inflammation. 3,4 The damages caused by radicals can be controlled by antioxidant compounds, which may have an endogenous origin, such as glutathione peroxidase, glutathione dismutase, catalase, and superoxide dismutase or exogenous, mainly supplied by diet and the use of topical products. 12 The main classes of compounds with antioxidant activity include vitamins, carotenoids, and phenolic compounds.…”

Section: Free R Ad I C Al S and Their Ac Ti On On E Arly Ag Ingmentioning

confidence: 99%

“…Reactive oxygen species take part in several degenerative processes to the organism . Epidemiological and experimental studies have provided an effective evidence of a critical association between free radicals and the development of early aging . Some applied antioxidants are able to block the harmful effects of free radicals as a prophylactic measure, resulting in normal production of structural proteins of the skin .…”

Section: Introductionmentioning

confidence: 99%

Polyphenols as natural antioxidants in cosmetics applications

Cherubim

Martins

Fariña

et al. 2019

J of Cosmetic Dermatology

206

View full text Add to dashboard Cite

Reactive oxygen species take part in several degenerative processes to the organism. 1 Epidemiological and experimental studies have provided an effective evidence of a critical association between free radicals and the development of early aging. 2,3 Some applied antioxidants are able to block the harmful effects of free radicals as a prophylactic measure, resulting in normal production of structural proteins of the skin. 1,4 Polyphenolic compounds are substances widely distributed in nature, related to the plant survival and its species perpetuation. Several studies associated the chemical structure of phenolic compounds with antioxidant activity. Phenolic nucleus presence acts out as an efficient sensor of reactive species, which also reduces and chelates ferric ions that catalyze lipid peroxidation. 5

show abstract

“…Supplementation of topical antioxidant is an attractive approach for prevention and treatment oxidative stress-mediated skin diseases 14 . For example, using in-vivo animal model, Cuelho et al, 2018 15 provided evidence that topical Yerba mate extracts rich in polyphenols, flavonoids, and tannins formulation having antioxidant activity was protective against acute UV-B exposure as shown by a decreased in myeloperoxidase and metalloproteinase-2 activities. Recently, beneficial effects of topical antioxidant have been shown by a non-invasive method on the volar forearm of the 25 Japanese volunteers 14 .…”

Section: Fig 4: In-vitro Shed Snake Skin Penetration Of Garcinia Mangmentioning

confidence: 99%

Untitled

2019

IJPSR

View full text Add to dashboard Cite

Garcinia mangostana L. pericarp (GMP) has been known as a traditional medicine in Asian countries. The bioactive from GMP has been recognized to be related to complex phenolic compounds linked with free radical scavenging activity. This research aimed to study the effects of topical formulation types (gel, oil in water cream and water in oil cream) of GMP extract on the in-vitro release and in-vitro shed snake skin penetration. The formulations were evaluated for viscosity and pH. The extent of GMP extracts released and penetrated shed snake skin were evaluated based on stable radical 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and expressed by Radical Scavenging activity equivalent GMP Extract concentration (RSGMPE). Mangostin skin penetration was quantified using UV spectrophotometric method. GMP extract exhibited significantly higher DPPH scavenging activity compared to α-mangostin which is known as the major constituent in the GMP extract. RSGMPE released and penetrated through the skin was significantly influenced by the type of formulations. GMP extract loaded gel provided highest RSGMPE amount transported through the skin than that retained in the shed snake skin membrane. On the contrary, total mangostin was found more retained in the shed snake skin membrane. When loaded in the gel, pure α-mangostin showed similar invitro shed snake skin penetration profile. GMP extract is potential to be developed used for topical applications with suitable formulation strategy for skin targetting.

show abstract

Topical formulation containing Ilex Paraguariensis extract increases metalloproteinases and myeloperoxidase activities in mice exposed to UVB radiation

Cited by 18 publications

References 43 publications

Inhibition of Prostate Cancer Cells by 4,5-Dicaffeoylquinic Acid through Cell Cycle Arrest

Inhibition of Prostate Cancer Cells by 4,5-Dicaffeoylquinic Acid through Cell Cycle Arrest

Polyphenols as natural antioxidants in cosmetics applications

Untitled

Contact Info

Product

Resources

About