Tomato Chlorotic Spot Virus (TCSV) Putatively Incorporated a Genomic Segment of Groundnut Ringspot Virus (GRSV) Upon a Reassortment Event

Silva, João Marcos Fagundes; Oliveira, A. S. de; Almeida, Mariana Martins Severo de; Kormelink, Richard; Nagata, Tatsuya; Resende, Renato O.

doi:10.3390/v11020187

Cited by 8 publications

(8 citation statements)

References 31 publications

(39 reference statements)

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“…It is worth mentioning that GRSV isolates grouped in two distinct clusters, one containing only GRSV isolates and another in which GRSV isolates intercalate with TCSV isolates. These observations confirm the recent proposal by Silva et al (2019) that TCSV represents the first reported interspecific reassortant orthotospovirus genome.…”

Section: Phylogenetic Analyses Based On the M Rna Segmentsupporting

confidence: 92%

“…New reassortant genomes were detected neither in Brazil nor in the Dominican Republic by analyzing the genome composition of the orthotospovirus isolates. However, based on the recent work performed by Silva et al (2019), the TCSV found in this study, most likely is a reassortant species.…”

Section: Phylogenetic Analyses Based On the M Rna Segmentsupporting

confidence: 49%

“…Using the primers designed for specific detection of the L, M and S segments of GRSV, TCSV and TSWV (Table 1), no new (reassortant) genome rearrangement was detected among the orthotospoviruses assessed. However, the recently proposed reassortant TCSV genome (Silva et al 2019) was present in both countries. It is worth noting that out of 62 samples that were collected in San Jose de Ocoa in the Dominican Republic and further analyzed, 17 from tomato plants and 15 from sweet pepper plants were positive for both PVY and TSWV, as confirmed by ELISA and/or RT-PCR (Table 3).…”

Section: Resultsmentioning

confidence: 96%

“…NSm genes of TSWV isolates were also included for comparison. By using the GRSV and TCSV genome sequences previously made available by Silva et al (2019), phylogenetic trees were built with protein sequences derived from the M RNA segments. In the trees based on protein sequences of the S and L segments, TCSV and GRSV isolates clearly clustered separately, forming two independent groups (data not shown).…”

Section: Phylogenetic Analyses Based On the M Rna Segmentmentioning

confidence: 99%

“…According to recent studies, the evolution of orthotospoviruses was mainly driven by the occurrence of intra-and inter-specific viral genome rearrangements for adaptation to different environmental conditions (Margaria et al 2007;Webster et al 2011Webster et al , 2015Tentchev et al 2011;Silva et al 2019). In attempting to develop defense strategies against phytosanitary problems, knowledge of the causal agents and their variability is indispensable, which in turn is crucial for development of efficient tools to elucidate their biological characteristics and their interactions in the environment.…”

Section: Introductionmentioning

confidence: 99%

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Analyses of orthotospovirus populations and dispersion under different environmental conditions in Brazil and in the Dominican Republic

Martínez

Almeida

Rodriguez³

et al. 2019

Trop. plant pathol.

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Orthotospoviruses (genus Orthotospovirus, family Tospoviridae) are amongst the most devastating plant viruses worldwide, causing severe damage to many economically important vegetable crops, such as tomato and sweet pepper. Monitoring virus populations is an important step for estimating virus damage and epidemiology, and gaining insights into the adaptation processes undergone by orthotospoviruses. Here, we studied the orthotospovirus populations infecting vegetable crops in Brazil and the Dominican Republic, including species diversity, genome comparison and phylogenetic analyses. Comparisons of virus populations showed that in Brazil, which is considered a center of orthotospovirus diversity, groundnut rinspot virus (GRSV) is prevalent, infecting 41% of the plants, whereas tomato spotted wilt virus (TSWV) and tomato chlorotic spot virus (TCSV) were present in 4% and 9% of the samples, respectively. In the Dominican Republic, which can be considered an environment with low orthotospovirus diversity, 55% of the samples were infected with TSWV, 11% showed TCSV infection and no GRSV was detected. The occurrence of mixed infection was low in Brazil, at only 5%, but no mixed infection was detected in the Dominican Republic. The low rates of mixed infections may prevent the emergence of genomes resulting from reassortment. Indeed, no reassortant viruses were detected in either country, except for TCSV, recently proposed as representing a reassortant orthotospovirus species.

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Section: Phylogenetic Analyses Based On the M Rna Segmentsupporting

confidence: 92%

Section: Phylogenetic Analyses Based On the M Rna Segmentsupporting

confidence: 49%

Section: Resultsmentioning

confidence: 96%

Section: Phylogenetic Analyses Based On the M Rna Segmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Analyses of orthotospovirus populations and dispersion under different environmental conditions in Brazil and in the Dominican Republic

Martínez

Almeida

Rodriguez³

et al. 2019

Trop. plant pathol.

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Orthotospovirus disease epidemic: molecular characterization and incidence in peanut crops

Breuil¹,

Dottori²,

Bejerman³

et al. 2020

J Plant Pathol

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Natural Infection of Cichorium intybus (Asteraceae) by Groundnut Ringspot Virus (Genus Orthotospovirus) Isolates in Brazil

et al. 2022

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Leaf chicory (Cichorium intybus L.) is a nutritionally rich vegetable used in regional cuisine in Brazil. Plants of C. intybus displaying symptoms (viz. chlorotic and necrotic ringspots, mosaic, and leaf deformation) similar to that induced by orthotospoviruses (genus Orthotospovirus, family Tospoviridae) were observed in three fields (≈ 0.2 ha each) in Gama County, in the Federal District, Brazil, from September 2016 to January 2020 in plants of the cultivars ‘Folha-Larga’ and ‘Spadona’ (Fig. 1). Incidence of symptomatic plants was nearly 10% in each field. Transmission electron microscopic examination of thin sections from symptomatic leaf samples showed typical membrane-bounded orthotospovirus particles within cisternae of spongy parenchymal cells (Fig 2). Two individual leaf samples per field were collected and submitted to dot enzyme-linked immunosorbent assay with polyclonal antisera against N protein of tomato spotted wilt virus (TSWV), groundnut ringspot virus (GRSV) and tomato chlorotic spot virus (TCSV). Symptomatic samples strongly reacted only against GRSV antibodies. Total RNA was extracted (Trizol®, Sigma) from all six samples and used as template in RT-PCR assays. The primer J13 (5’-CCCGGATCCAGAGCAAT-3’) was employed for cDNA synthesis using M-MLV reverse transcriptase. PCR assays were done with the primer pair BR60/BR65 (Eiras et al., 2001) to obtain ≈ 500 bp fragment of untranslated region and partial N gene in the S RNA segment from each sample. Purified RT-PCR products of two randomly selected individual samples were directly sequenced (GenBank MW467981 and MZ126602) and their BLASTn analyses displayed 99 to 100% nucleotide identity to GRSV isolates previously reported infecting C. endivia L. in Brazil (Jorge et al., 2021). Our analyses combining N protein serology and N-gene sequencing (both directed to the S RNA segment) allowed us to confirm the GRSV infection of C. intybus, but the potential reassortant nature of these isolates (Webster et al., 2015; Silva et al., 2019) are unknown since their M RNA segments were not characterized. Individual leaf extracts (in phosphate buffer, pH 7.0) of the sequenced isolates were mechanically inoculated onto ten seedlings of two C. intybus cultivars (‘Folha Larga’ and ‘Pão-de-Açúcar’) and three plants each of the indicator hosts Capsicum chinense PI 159236, Nicandra physalodes; Nicotiana rustica; Datura stramonium; and tomato cv. Santa Clara. Systemic chlorotic and necrotic ringspots, mosaic, and leaf deformation developed in the indicator hosts and infection by GRSV was confirmed via serological assays 20 days after inoculation. However, no symptoms and no serological reaction to GRSV antibodies were observed on the C. intybus cultivars even after two successive mechanical inoculations. This transmission failure might be due to factors such as the requirement of the thrips vector(s), physicochemical barriers in the foliage or the presence of non-mechanically transmissible helper agent(s) necessary to ensure GRSV infection of C. intybus. The natural infection of C. intybus by a not fully characterized orthotospovirus (mostly likely TSWV) has been observed since 1938 in Brazil (Kitajima, 2020). Our report of GRSV infecting C. intybus is thus confirming previous speculations that similar symptoms in this vegetable crop were induced by orthotospovirus infection in Brazil. References: Eiras, M. et al. 2001. Fitopatol. Bras. 26: 170. Jorge, T. S. et al. 2021. Plant Dis. 105: 714. Kitajima, E.W. 2020. Biota Neotrop. 20: e2019932. Silva, J. M. F. et al. 2019. Viruses 11: 187. Webster, C.G. et al. 2015. Phytopathology 105: 388.

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Tomato Chlorotic Spot Virus (TCSV) Putatively Incorporated a Genomic Segment of Groundnut Ringspot Virus (GRSV) Upon a Reassortment Event

Cited by 8 publications

References 31 publications

Analyses of orthotospovirus populations and dispersion under different environmental conditions in Brazil and in the Dominican Republic

Analyses of orthotospovirus populations and dispersion under different environmental conditions in Brazil and in the Dominican Republic

Orthotospovirus disease epidemic: molecular characterization and incidence in peanut crops

Natural Infection of Cichorium intybus (Asteraceae) by Groundnut Ringspot Virus (Genus Orthotospovirus) Isolates in Brazil

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