ToF‐SIMS spectrometry to observe fatty acid profiles of breast tissues in broiler chicken subjected to varied vegetable oil diet

Abstract

This study is aimed to observe changes in fatty acid profiles by time of flight secondary ion mass spectrometry (ToF‐SIMS) in breast muscle tissues of broilers. Four different groups were identified. The source of fat in group I was soy oil (rich in linoleic acid, ω‐6), group II received linseed oil (ω‐3), and the third group was fed a mixture of the two mentioned oils. Broilers in the control group were fed with beef tallow, used in mass commercial production. The results reveal that the use of vegetable oils in animal nutrition determines the lipid profile of fatty acids. ToF‐SIMS measurements showed that the lipid profile of muscle fibers and intramuscular fat reflect the composition of fats used as feed additives. In both structures, the ratio of ω‐6/ω‐3 fatty acids, which is most favorable for human health, was found in the groups in which a mixture of vegetable oils and a supplement of linseed oil were used.

Correlations between first order echotextural characteristics and chemical composition of pectoralis major muscles in broiler chickens receiving different dietary fat supplements

“…At present, the assessment of meat quality in poultry facilities is typically carried out post-mortem. This allows for high accuracy but restricts the ability of the producers to make "real-time decisions" and improve meat quality using nutritional and sanitary or technological adjustments (Pena-Gonzalez, 2017;Bogucka et al, 2018;Sugiharto et al, 2018;Marzec et al, 2020). Moreover, both the quality control in commercial farms as well as genetic selection of birds require constant monitoring of meat productivity traits; phenotypic data combined with whole genome data can provide "deeper insight into the control of growth and body/carcass composition of farm animals" and "add extra value for genomic selection" (Petracci and Baeza, 2010).…”

Advancements in ToF-SIMS imaging for life sciences

“…This capability has been demonstrated in several studies, where ToF-SIMS was applied to visualize the metabolites in different cell types and identify potential biomarkers for cell type classification, such as unsaturated diacylglycerol, oleic acid, and linoleic acid, in combination with immunofluorescence ( Song et al, 2020 ). Another study investigated fatty acid profiles in breast muscle tissues of broilers, revealing the impact of fed additives on the composition of fats in the tissues ( Marzec et al, 2020 ).…”

Analytical Methodologies for Agrometallomics: A Critical Review

“…For another, TOF-SIMS not only renders inorganic elemental information but also reflects organic composition characteristics. Cryo-TOF-SIMS analysis was employed to verify the distribution of Al, K, hydrangea blue-complex, and copigment in sepal tissues of one plant; TOF-SIMS was proposed to show the lipid profile of muscle fibers and intramuscular fat reflecting the composition of fats used as feed additives or the information on both volatile and nonvolatile compounds to classify the characterization of coffee . In addition, SIMS also reveals the diverse isotopic composition or ratio information , as well as chemical speciation of different oxidation states of some transition metals …”