Advancements in ToF-SIMS imaging for life sciences

Jia, Feifei; Zhao, Xia; Zhao, Yao

doi:10.3389/fchem.2023.1237408

Cited by 12 publications

(5 citation statements)

References 139 publications

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“…Advances in high-throughput metabolomics by mass spectrometry are in high demand in large-scale pharmaceutical and medical studies . ToF-SIMS has become a promising analytical tool of choice as a high-sensitive and label-free method for metabolomics and lipidomics in life sciences . Biofluids, such as blood plasma, serum, urine, salvia, etc., are collected efficiently and noninvasively from patients and widely used for metabolomic profiling to diagnose and monitor diseases. , Also, absolute quantitation is highly desirable for accurate disease diagnostics.…”

Section: Resultsmentioning

confidence: 99%

High-Throughput Quantitative Analysis of Amino Acids in Freeze-Dried Drops Using Time-of-Flight Secondary Ion Mass Spectrometry

Lim,

Lee,

Jin

et al. 2024

Anal. Chem.

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Time-of-flight secondary ion mass spectrometry (ToF-SIMS) has become a promising analytical tool for molecular profiling in biological applications. However, its ultrahigh vacuum environment and matrix effects hamper the absolute quantitation of solution samples. Herein, we present a rapid high-throughput platform for quantitative ToF-SIMS analysis of amino acids in matrix deposits formed from freeze-dried solution drops through ice sublimation on a parylene film microarray substrate. Droplets of the amino acid solutions, which were mixed with stable isotopelabeled phenylalanine (F*) of high concentration (10 mM), were loaded on wells of the microarray, then frozen and evaporated slowly below the freezing point, forming continuous solid-phase F* matrix deposits. The amino acids (≤500 μM), adequately well dispersed throughout the F* matrix deposits on each well, were quantitatively analyzed by ToF-SIMS in a rapid and high-throughput fashion. The lower limit of quantitation reached below 10 μM.

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Section: Resultsmentioning

confidence: 99%

High-Throughput Quantitative Analysis of Amino Acids in Freeze-Dried Drops Using Time-of-Flight Secondary Ion Mass Spectrometry

Lim,

Lee,

Jin

et al. 2024

Anal. Chem.

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“…Despite the fact that applications of SIMS in lipidomics starts much later than MALDI, developments in SIMS such as introduction of nanoSIMS or cluster ion beams (i.e., Bi 3+ Au 3+ , Au 9+ ) that are able to reduce secondary ions fragmentation has led to improved spatial resolution and analytical sensitivity [59,80,81]. Advances in TOF-SIMS were recently reviewed in detail by Jia et al [82] and the important role of SIMS in lipid imaging was recently proven by Ren et al [83]. A single-cell lipidomic study was performed using TOF-SIMS analysis of mammalian cells (cardiomyocytes (CMs)).…”

Section: Mass Spectrometry Imagingmentioning

confidence: 99%

Recent Analytical Methodologies in Lipid Analysis

Gerhardtova,

Jankech,

Majerova

et al. 2024

IJMS

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Lipids represent a large group of biomolecules that are responsible for various functions in organisms. Diseases such as diabetes, chronic inflammation, neurological disorders, or neurodegenerative and cardiovascular diseases can be caused by lipid imbalance. Due to the different stereochemical properties and composition of fatty acyl groups of molecules in most lipid classes, quantification of lipids and development of lipidomic analytical techniques are problematic. Identification of different lipid species from complex matrices is difficult, and therefore individual analytical steps, which include extraction, separation, and detection of lipids, must be chosen properly. This review critically documents recent strategies for lipid analysis from sample pretreatment to instrumental analysis and data interpretation published in the last five years (2019 to 2023). The advantages and disadvantages of various extraction methods are covered. The instrumental analysis step comprises methods for lipid identification and quantification. Mass spectrometry (MS) is the most used technique in lipid analysis, which can be performed by direct infusion MS approach or in combination with suitable separation techniques such as liquid chromatography or gas chromatography. Special attention is also given to the correct evaluation and interpretation of the data obtained from the lipid analyses. Only accurate, precise, robust and reliable analytical strategies are able to bring complex and useful lipidomic information, which may contribute to clarification of some diseases at the molecular level, and may be used as putative biomarkers and/or therapeutic targets.

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“…The Giant Gas Cluster Ion Beam-SIMS (GCIB-SIMS), leveraging its capability to capture molecular ions or substantial fragment ions, has demonstrated remarkable potential for mapping intact biomolecules within tissue and cell samples with a much higher mass range [36]. GCIB-SIMS operates by employing giant cluster ions as the primary ion beam, with these clusters typically comprising hundreds or even thousands of atoms and molecules.…”

Section: Sims Principlesmentioning

confidence: 99%

Secondary Ion Mass Spectral Imaging of Metals and Alloys

Shen,

Howard,

2024

Materials

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Secondary Ion Mass Spectrometry (SIMS) is an outstanding technique for Mass Spectral Imaging (MSI) due to its notable advantages, including high sensitivity, selectivity, and high dynamic range. As a result, SIMS has been employed across many domains of science. In this review, we provide an in-depth overview of the fundamental principles underlying SIMS, followed by an account of the recent development of SIMS instruments. The review encompasses various applications of specific SIMS instruments, notably static SIMS with time-of-flight SIMS (ToF-SIMS) as a widely used platform and dynamic SIMS with Nano SIMS and large geometry SIMS as successful instruments. We particularly focus on SIMS utility in microanalysis and imaging of metals and alloys as materials of interest. Additionally, we discuss the challenges in big SIMS data analysis and give examples of machine leaning (ML) and Artificial Intelligence (AI) for effective MSI data analysis. Finally, we recommend the outlook of SIMS development. It is anticipated that in situ and operando SIMS has the potential to significantly enhance the investigation of metals and alloys by enabling real-time examinations of material surfaces and interfaces during dynamic transformations.

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Advancements in ToF-SIMS imaging for life sciences

Cited by 12 publications

References 139 publications

High-Throughput Quantitative Analysis of Amino Acids in Freeze-Dried Drops Using Time-of-Flight Secondary Ion Mass Spectrometry

High-Throughput Quantitative Analysis of Amino Acids in Freeze-Dried Drops Using Time-of-Flight Secondary Ion Mass Spectrometry

Recent Analytical Methodologies in Lipid Analysis

Secondary Ion Mass Spectral Imaging of Metals and Alloys

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