Tobramycin

Dash, Alekha K.

doi:10.1016/s0099-5428(08)60703-0

Cited by 12 publications

(9 citation statements)

References 40 publications

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“…The WAXS curve (Figure , inset) for the PE/TbS complex showed broad maxima at Bragg spacing of 0.45 nm, which is consistent with a liquidlike ordering of phenyl groups. The broadened and low-intensity peaks also suggests that the two complexes were amorphous . Combining the X-ray scattering data (molecular ordering) and TEM observation (vesicle formation) for the two complexes, it is evident that the backbone hydrophobicity plays an important role in the determining biaqueous vesicle characteristics.…”

Section: Resultsmentioning

confidence: 90%

From Ultrathin Capsules to Biaqueous Vesicles

et al. 2005

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The layer-by-layer (LbL) adsorption of anionic polyelectrolytes (PE) and tobramycin sulfate (TbS) multilayers on zinc oxide core particles followed by the controlled core-removal process leads to the formation of ultrathin capsules, which gradually convert to biaqueous vesicles and emulsionlike systems depending on the hydrophilicity/hydrophobicity of the PE backbone, PE/TbS ratio, and Zn2+ concentration. The unique characteristics of the PE/TbS multilayer capsules result because of the formation of PE/TbS/H2O biphasic liquid systems unlike the other LbL capsular systems that form stiff PE coacervates when mixed together in water. This paper investigates the PE/TbS ultrathin capsule to biaqueous vesicle transition and its physicochemical properties.

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Section: Resultsmentioning

confidence: 90%

From Ultrathin Capsules to Biaqueous Vesicles

et al. 2005

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“…The very low absorptivity of TOB does not permit its direct quantification at low concentrations, particularly if there is need to estimate the drug in plasma or in biological fluids. 20 This problem could be solved by derivatizing the compound by a suitable fluorogenic reagent. Fluorescamine offers enhanced fluorescence detection of primary amine.…”

Section: Resultsmentioning

confidence: 99%

“…Chemically, TOB consists of amino sugars linked glycosidically with 1,3-diaminocyclohexane central ring 19. Like most carbohydrates, TOB lacks UV absorbing chromophores and does not possess native fluorescence, leading to a major challenge in the analysis of such a compound due to problematic detection 20. Therefore, derivatization with a suitable absorbance- enhancing or fluorescence-producing agent is required for the detection by chromatographic techniques.…”

Section: Introductionmentioning

confidence: 99%

“…19 Like most carbohydrates, TOB lacks UV absorbing chromophores and does not possess native fluorescence, leading to a major challenge in the analysis of such a compound due to problematic detection. 20 Therefore, derivatization with a suitable absorbance-enhancing or fluorescence-producing agent is required for the detection by chromatographic techniques. HPLC methods with fluorescence detection, after derivatization 21 or indirect fluorescence detection, based on ligand displacement 22 were previously employed.…”

Section: Introductionmentioning

confidence: 99%

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Utility of Experimental Design in Pre-Column Derivatization for the Analysis of Tobramycin by HPLC—Fluorescence Detection: Application to Ophthalmic Solution and Human Plasma

El‐Zaher

Mahrouse

2013

Anal Chem�Insights

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A novel, selective, and sensitive reversed phase high-performance liquid chromatography (HPLC) method coupled with fluorescence detection has been developed for the determination of tobramycin (TOB) in pure form, in ophthalmic solution and in spiked human plasma. Since TOB lacks UV absorbing chromophores and native fluorescence, pre-column derivatization of TOB was carried out using fluorescamine reagent (0.01%, 1.5 mL) and borate buffer (pH 8.5, 2 mL). Experimental design was applied for optimization of the derivatization step. The resulting highly fluorescent stable derivative was chromatographed on C18 column and eluted using methanol:water (60:40, v/v) at a flow rate of 1 mL min−1. A fluorescence detector (λex 390 and λem 480 nm) was used. The method was linear over the concentration range 20–200 ng mL−1. The structure of the fluorescent product was proposed, the method was then validated and applied for the determination of TOB in human plasma. The results were statistically compared with the reference method, revealing no significant difference.

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“…Thus, it is strongly basic and exists as polycations at physiologic pH and as sulfates in pharmaceutical formulations [15]. Its broad spectrum of activity against many common pathogens of both Gram-positive and Gram-negative types was reported [16]. The mechanism of uptake of aminoglycosides across the outer membrane of E. coli was reevaluated [17].…”

Section: Introductionmentioning

confidence: 99%

Potentiometric assessment of Gram-negative bacterial permeabilization of tobramycin

El-Kosasy

2006

Journal of Pharmaceutical and Biomedical Analysis

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Tobramycin

Cited by 12 publications

References 40 publications

From Ultrathin Capsules to Biaqueous Vesicles

From Ultrathin Capsules to Biaqueous Vesicles

Utility of Experimental Design in Pre-Column Derivatization for the Analysis of Tobramycin by HPLC—Fluorescence Detection: Application to Ophthalmic Solution and Human Plasma

Potentiometric assessment of Gram-negative bacterial permeabilization of tobramycin

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