Potentiometric assessment of Gram-negative bacterial permeabilization of tobramycin

El-Kosasy, Amira M.

doi:10.1016/j.jpba.2006.04.022

Cited by 4 publications

(3 citation statements)

References 25 publications

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“…The proposed sensors were also compared with other electrochemical sensors from literature for determination of TOBRA [13,47], as shown in Table 2. This comparison shows that C-dots-CPE is superior to the reported aptamer-based gold sensor and β-Cyclodextrinbased sensor concerning the LOD, working pH range, lifetime, and intraday precision.…”

Section: Performance Characteristics Of the Bare-cpe Agnp-cpe And C-dots-cpementioning

confidence: 99%

“…They are composed of graphite powder, an electroactive material, and a liquid binder, with the addition of nanoparticles as a new modification [9]. A literature survey revealed that electrochemical determination of TO-BRA was mainly performed through voltammetric sensors [10][11][12], and to date there is only one potentiometric sensor for the direct determination of TOBRA through a classical ion-selective electrode, where the sensing membrane was composed of β-cyclodextrin as ionophore for the evaluation of permeability of TOBRA in gram-negative bacteria [13]. Additionally, no carbon paste electrodes, whether bare or modified with nanoparticles, have been used for electrochemical determination of TOBRA.…”

Section: Introductionmentioning

confidence: 99%

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Influence of Nitrogen-Doped Carbon Dot and Silver Nanoparticle Modified Carbon Paste Electrodes on the Potentiometric Determination of Tobramycin Sulfate: A Comparative Study

et al. 2021

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Two inexpensive and simple methods for synthesis of carbon nanodots were applied and compared to each other, namely a hydrothermal and microwave-assisted method. The synthesized carbon nanodots were characterized using transmission electron microscopy (TEM), ultraviolet-visible (UV-Vis), photoluminescence (PL), Fourier transform-infrared spectroscopy (FTIR), and X-ray diffraction (XRD). The synthesized microwave carbon nanodots had smaller particle size and were thus chosen for better electrochemical performance. Therefore, they were used for our modification process. The proposed electrodes performance characteristics were evaluated according to the IUPAC guidelines, showing linear response in the concentration range 10−6–10−2, 10−7–10−2, and 10−8–10−2 M of tobramycin with a Nernstian slope of 52.60, 58.34, and 57.32 mV/decade for the bare, silver nanoparticle and carbon nanodots modified carbon paste electrodes, respectively. This developed potentiometric method was used for quantification of tobramycin in its co-formulated dosage form and spiked human plasma with good recovery percentages and without interference of the co-formulated drug loteprednol etabonate and excipients.

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Section: Performance Characteristics Of the Bare-cpe Agnp-cpe And C-dots-cpementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Influence of Nitrogen-Doped Carbon Dot and Silver Nanoparticle Modified Carbon Paste Electrodes on the Potentiometric Determination of Tobramycin Sulfate: A Comparative Study

et al. 2021

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“…Working on the determination of tobramycin with biosensor method has been started since 1997 with RNA aptamers [97], but after 9 years, the researchers were able to detect tobramycin with potentiometric measurements only in aqueous solutions [98].…”

Section: Tobramycinmentioning

confidence: 99%

An Overview of Different Methods for Aminoglycoside Residue Determination

Morovatdar

Khameneh

Khashyarmanesh

et al. 2021

JPRI

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Aminoglycosides (AGs) are chemical substances that exist in the bacteriologic category of traditional antibiotic (AB) therapy. The importance of the determination of AG as has been described in many situations by researchers. Because of the narrow therapeutic ranges of AGs, considerable efforts have been attributed to the analysis of AGs in pharmaceutical preparations, serum, and urine specimens for therapeutic drug monitoring purposes. Residues of ABs in many different cases like environment and human food, causes a major concern, as prolonged exposure to ABs is a serious health hazard, related to both side effects of prolonged use and the risk of developing bacterial resistance to various ABs. The major challenge is finding a sensitive and reliable method to determine AGs in a complex matrix. The microbiological assay was a simple and old method for the determination of AGs. Chromatography and spectroscopy methods are the main instrumental methods for analysis that have been employed for these purposes. Biosensor based instrumental systems have been recently used to determine the AG residues in many cases. Each of these methods has its advantages and disadvantages. This review summarizes different ways (microbiological methods, instrumental methods, and biosensor) for the determination of AGs in all cases. Different databases including PubMed, Scopus, and Web of Science with the words of AGs determination and related words for antimicrobial keywords searched without time limitation.

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Fluorescent probes to evaluate the physiological state and activity of microbial biocatalysts: A guide for prokaryotic and eukaryotic investigation

Cao-Hoang

Marechal

Lê-Thanh

et al. 2008

Biotechnology Journal

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Many fluorescent techniques are employed to evaluate the viability and activity of microbial cells used in biotechnology. These techniques are sometimes complex and the interpretation of results opened to misunderstanding. Moreover, new developments are constantly proposed especially concerning a more accurate evaluation of the state of the cells including eukaryotic microorganisms. This paper aims at presenting to biotechnologists unfamiliar with fluorescence the principles of these methods and the related possible pitfalls. It focuses on probes of the physical (integrity and fluidity) and energetical (intracellular pH and membrane potential) state of the cell membrane (bacterial and yeast cells) and presents also other probes (nucleic acids, respiration...) and new technical trends. The specificities of Gram-negative bacterial cells are also discussed.

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Potentiometric assessment of Gram-negative bacterial permeabilization of tobramycin

Cited by 4 publications

References 25 publications

Influence of Nitrogen-Doped Carbon Dot and Silver Nanoparticle Modified Carbon Paste Electrodes on the Potentiometric Determination of Tobramycin Sulfate: A Comparative Study

Influence of Nitrogen-Doped Carbon Dot and Silver Nanoparticle Modified Carbon Paste Electrodes on the Potentiometric Determination of Tobramycin Sulfate: A Comparative Study

An Overview of Different Methods for Aminoglycoside Residue Determination

Fluorescent probes to evaluate the physiological state and activity of microbial biocatalysts: A guide for prokaryotic and eukaryotic investigation

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