2013
DOI: 10.4137/aci.s11612
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Utility of Experimental Design in Pre-Column Derivatization for the Analysis of Tobramycin by HPLC—Fluorescence Detection: Application to Ophthalmic Solution and Human Plasma

Abstract: A novel, selective, and sensitive reversed phase high-performance liquid chromatography (HPLC) method coupled with fluorescence detection has been developed for the determination of tobramycin (TOB) in pure form, in ophthalmic solution and in spiked human plasma. Since TOB lacks UV absorbing chromophores and native fluorescence, pre-column derivatization of TOB was carried out using fluorescamine reagent (0.01%, 1.5 mL) and borate buffer (pH 8.5, 2 mL). Experimental design was applied for optimization of the d… Show more

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Cited by 19 publications
(13 citation statements)
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“…Analytical methods for this substance are described in both the EP [12] and United States Pharmacopoeia (USP) [13] as well as in the literature. Derivatization and subsequent UV or fluorescence detection is a well-known approach for analyzing aminoglycosides [12,14,15,13,16,17]. There are, however, some major drawbacks with this approach including unstable products and/or incomplete derivatization.…”
Section: Tobramycin Sulfatementioning
confidence: 99%
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“…Analytical methods for this substance are described in both the EP [12] and United States Pharmacopoeia (USP) [13] as well as in the literature. Derivatization and subsequent UV or fluorescence detection is a well-known approach for analyzing aminoglycosides [12,14,15,13,16,17]. There are, however, some major drawbacks with this approach including unstable products and/or incomplete derivatization.…”
Section: Tobramycin Sulfatementioning
confidence: 99%
“…Frequently named ion-pair-reagents are sodium octanesulfonate [10] and fluorinated carboxylic acids like trifluoroacetic acid [21] or heptafluorobutyric acid (HFBA) [22,23]. Detection strategies include diode-array, fluorescence [12,14,15,13,16,17], refractometry [9], evaporative light scattering (ELSD) [21,23], mass spectrometry (MS) [24] and pulsed electrochemical detection (PED) [11,25]. Each of these detection methods has its own advantages and disadvantages.…”
Section: Tobramycin Sulfatementioning
confidence: 99%
“…The most common derivatizing agent used in the bioanalysis of aminoglycosides was o-phthalaldehyde; reported by 24 articles for the derivatization of gentamicin, amikacin, kanamycin, netilmicin, isepamicin, sisomicin, neomycin, tobramycin, dibekacin, astromicin, and micronomicin [6,14,20,[22][23][24][25][26]28,36,37,43,44,48,50,52,53,55,[57][58][59]61,62,66]. Dansyl chloride for the derivatization of netilmicin [21]; 1-fluoro-2,4-dinitrobenzene for the derivatization of geneticin, gentamicin, and amikacin [29,38,41,45,56,64]; 7-fluoro-4-nitrobenz-2-oxa-1,3diazole for the derivatization of amikacin [74]; benzene sulphonyl chloride for the derivatization of gentamicin [39]; 6-aminoquinolyl-N-hydroxysucciminidylcarbamate for the derivatization of isepamicin [76]; fluorescamine for the derivatization of gentamicin, and tobramycin [40,80]; 1-naphthyl isothiocyanate for the derivatization of amikacin, and tobramycin [65,67]; fluorescein isothiocyanate for the derivatization of tobramycin [77]; o-phthalicdicarboxaldehyde for the derivatization of gentamicin [42]; 2,4,6trinitrobenzene sulfonic a...…”
Section: Derivatization and Mode Of Detectionmentioning
confidence: 99%
“…therapeutic reference range [14,15,24,25,30,31,34,37,48,55,57,61,65,67,71,74,75,[80][81][82], which invalidates the measurements obtained from the bioanalysis.…”
Section: A D V a N C E D O N L I N E A R T I C L Ementioning
confidence: 99%
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