Purity determination of amphotericin B, colistin sulfate and tobramycin sulfate in a hydrophilic suspension by HPLC

Pfeifer, Corina; Fassauer, Georg M.; Gerecke, Hagen; Jira, Thomas; Remane, Yvonne; Frontini, Roberto; Byrne, Jonathan; Reinhardt, Robert M.

doi:10.1016/j.jchromb.2015.02.043

Cited by 23 publications

(7 citation statements)

References 20 publications

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“…Tobramycin, colistin, and AA-230 are all hydrophilic antimicrobials and have similar positive charges at physiological pH (21)(22)(23). The molecular weight of tobramycin is 468 g/mol, that of colistin is 1,156 g/mol, and that of AA-230 is 2,578 g/mol; this makes it interesting to compare bacterial killing by these antimicrobials at equivalent molar concentrations.…”

Section: Discussionmentioning

confidence: 99%

Role of Viscoelasticity in Bacterial Killing by Antimicrobials in Differently Grown Pseudomonas aeruginosa Biofilms

Rozenbaum

Mei

Woudstra

et al. 2019

Antimicrob Agents Chemother

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Pseudomonas aeruginosa colonizes the sputum of most adult cystic fibrosis patients, forming difficult-to-eradicate biofilms in which bacteria are protected in their self-produced extracellular polymeric substance (EPS) matrices. EPS provide biofilms with viscoelastic properties, causing time-dependent relaxation after stress-induced deformation, according to multiple characteristic time constants. These time constants reflect different biofilm (matrix) components. Since the viscoelasticity of biofilms has been related to antimicrobial penetration but not yet bacterial killing, this study aims to relate killing of P. aeruginosa, in its biofilm mode of growth, by three antimicrobials to biofilm viscoelasticity. P. aeruginosa biofilms were grown for 18 h in a constant-depth film fermenter, with mucin-containing artificial sputum medium (ASM+), artificial sputum medium without mucin (ASM−), or Luria-Bertani (LB) broth; this yielded 100-μm-thick biofilms that differed in their amounts of matrix environmental DNA (eDNA) and polysaccharides. Low-load compression testing, followed by three-element Maxwell analyses, showed that the fastest relaxation component, associated with unbound water, was most important in LB-medium-grown biofilms. Slower components due to water with dissolved polysaccharides, insoluble polysaccharides, and eDNA were most important in the relaxation of ASM+-grown biofilms. ASM−-grown biofilms showed intermediate stress relaxation. P. aeruginosa in LB-medium-grown biofilms was killed most by exposure to tobramycin, colistin, or an antimicrobial peptide, while ASM+ provided the most protective matrix, with less water and most insoluble polysaccharides and eDNA. In conclusion, stress relaxation of P. aeruginosa biofilms grown in different media revealed differences in matrix composition that, within the constraints of the antimicrobials and growth media applied, correlated with the matrix protection offered against different antimicrobials.

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Section: Discussionmentioning

confidence: 99%

Role of Viscoelasticity in Bacterial Killing by Antimicrobials in Differently Grown Pseudomonas aeruginosa Biofilms

Rozenbaum

Mei

Woudstra

et al. 2019

Antimicrob Agents Chemother

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“…Polymyxins E1 and E2 contain the same amino acids but differ in their fatty acid components (6-methyl-octanoic acid and 6-methyl-heptanoic acid, respectively). Different pharmaceutical formulations can contain different amounts of these two components [13,[30][31][32][33][34][35]. For pharmaceutical use, polymyxin E is available in the form of colistin sulfate (topical and oral use) and as sodium colistimethate (injection and inhalation).…”

Section: Chemical Structure Of Polymyxinsmentioning

confidence: 99%

Polymyxin Delivery Systems: Recent Advances and Challenges

Dubashynskaya

Skorik

2020

Pharmaceuticals

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Polymyxins are vital antibiotics for the treatment of multiresistant Gram-negative ESKAPE pathogen infections. However, their clinical value is limited by their high nephrotoxicity and neurotoxicity, as well as their poor permeability and absorption in the gastrointestinal tract. This review focuses on various polymyxin delivery systems that improve polymyxin bioavailability and reduce drug toxicity through targeted and controlled release. Currently, the most suitable systems for improving oral, inhalation, and parenteral polymyxin delivery are polymer particles, liposomes, and conjugates, while gels, polymer fibers, and membranes are attractive materials for topical administration of polymyxin for the treatment of infected wounds and burns. In general, the application of these systems protects polymyxin molecules from the negative effects of both physiological and pathological factors while achieving higher concentrations at the target site and reducing dosage and toxicity. Improving the properties of polymyxin will be of great interest to researchers who are focused on developing antimicrobial drugs that show increased efficacy and safety.

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“…HPLC has been widely used since it is a more sensitive and precise technique than a microbiological bioassay. However, its disadvantage lies in being a test which requires expensive equipment, high reagent consumption, sample pre-treatment, solid phase extraction and derivatisation which requires specialised personnel [108,109]. Difficulties have arisen regarding analysing and quantifying the mixture of CMS compounds in its commercial presentation, thereby hindering their separation [100].…”

Section: Colistin Plasma Level Measurementsmentioning

confidence: 99%

An Approach to Measuring Colistin Plasma Levels Regarding the Treatment of Multidrug-Resistant Bacterial Infection

et al. 2019

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Antimicrobial resistance to antibiotic treatment has significantly increased during recent years, causing this to become a worldwide public health problem. More than 70% of pathogenic bacteria are resistant to at least one of the currently used antibiotics. Polymyxin E (colistin) has recently been used as a “last line” therapy when treating Gram-negative multi-resistant bacteria. However, little is known about these molecules’ pharmacological use as they have been discontinued because of their high toxicity. Recent research has been focused on determining colistimethate sodium’s pharmacokinetic parameters to find the optimal dose for maintaining a suitable benefit–risk balance. This review has thus been aimed at describing the use of colistin on patients infected by multi-drug resistant bacteria and the importance of measuring this drug’s plasma levels in such patients.

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Purity determination of amphotericin B, colistin sulfate and tobramycin sulfate in a hydrophilic suspension by HPLC

Cited by 23 publications

References 20 publications

Role of Viscoelasticity in Bacterial Killing by Antimicrobials in Differently Grown Pseudomonas aeruginosa Biofilms

Role of Viscoelasticity in Bacterial Killing by Antimicrobials in Differently Grown Pseudomonas aeruginosa Biofilms

Polymyxin Delivery Systems: Recent Advances and Challenges

An Approach to Measuring Colistin Plasma Levels Regarding the Treatment of Multidrug-Resistant Bacterial Infection

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