TNF-α and TGF-β Contributes in Recurrent Otorrhea of  Active Mucosal Chronic Otitis Media

Pratiwi, Dewi; Rizqiana, Marisa; Wicaksono, Adisetya; Permatasari, Defitaria; Restuti, Ratna Dwi; Susilawati, Tri Nugraha; Sutarno, Sutarno

doi:10.18585/inabj.v14i1.1759

Cited by 4 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(36,37) In addition, increased levels of TNF-a also play a role in the pathophysiology of other diseases that increase the inflammatory process. (38,39) We found that, in contrast to a-defensins protein expression which was decreased, IL-1b protein expression was increased during the study and the increase was significant at the end of the study as compared to that of normal-control rats. This indicates that the inflammatory process in HAEC will continue as a result of a decrease in the protein expression of a-defensins, the antimicrobial peptide.…”

Section: Discussionmentioning

confidence: 74%

Paneth Cell Hyperplasia and Metaplasia in Hirschsprung-associated Enterocolitis in An Aganglionosis Rat Model

et al. 2022

View full text Add to dashboard Cite

BACKGROUND: Many hypotheses regarding the pathophysiology of enterocolitis in aganglionic megacolon or Hirschsprung disease (HSCR) has been proposed. Paneth cells are columnar intestinal epithelial cells that have an important role in maintaining of intestinal homeostasis as a bactericide. Since enterocolitis in HSCR may have association with Paneth cells metaplasia and hyperplasia, current study investigated Paneth cells metaplasia and hyperplasia in the sigmoid colon of HSCR rat model and its products, namely a-defensins and IL-1b, in the sigmoid colon tissues.METHODS: Aganglionosis-induced and control Sprague-Dawley rats were euthanized on Day (D)-7, -14, -17, -19, -21, -23, -25, and -28. Sigmoid colon tissue was isolated at each time point, and degree of enterocolitis as well as Paneth cells metaplasia and hyperplasia were analyzed by Hematoxylin-eosin staining, then protein levels of a-defensins and interleukin (IL)-1b were determined by enzyme-linked immunosorbent assay (ELISA).RESULTS: Enterocolitis scores increased with time. The Paneth cells metaplasia and hyperplasia were observed on D14 until D28 (p<0.01 vs. control group) followed by an increased in the levels of IL-1b. The levels of a-defensins protein expression were initially increased (D7-D14; p<0.01 vs. control group) but then undergo reciprocal changes on D19 until D28 (p<0.01 vs. D7 and D14). Positive correlations between the degree of enterocolitis and Paneth cells number were detected in the sigmoid colon (r=0.42).CONCLUSION: Paneth cells underwent metaplasia and hyperplasia in the sigmoid colon of HSCR rats corresponding to an increase in the degree of enterocolitis, but not followed by an increase in the level of a-defensins as well as IL-1b, suggesting that there is an involvement of Paneth cells in the pathophysiology of enterocolitis due to HSCR.KEYWORDS: Hirschsprung, enterocolitis, defensins; metaplasia, Paneth cell, animal model

show abstract

Section: Discussionmentioning

confidence: 74%

Paneth Cell Hyperplasia and Metaplasia in Hirschsprung-associated Enterocolitis in An Aganglionosis Rat Model

et al. 2022

View full text Add to dashboard Cite

show abstract

“…(17) This is in line with the study on TNF-α which are proinflammatory and immune regulatory cytokines, may contribute to the pathogenesis of recurrent inflammatory in an otitis media suppurative chronic. (18) In our study there was a significant difference between the average levels of TNF-α in the treatment group and the control group. So, it is postulated that the administration The level of p53 in the treatment group was higher than in the control group.…”

Section: Discussionmentioning

confidence: 42%

Purple Sweet Potato Reduces Malondialdehyde and TNF-a, Increases p53, and Protects Histopathological Appearance in Formaldehyde-induced Nasopharyngeal Carcinoma Rats

Susilawati¹,

Yasa

Suardana

et al. 2022

Indones Biomed J

View full text Add to dashboard Cite

BACKGROUND:Purple sweet potato tuber ethanol extract (PSPTEE) has been known to have benefits in various disease, including to prevent nasopharyngeal carcinoma (NPC). However, there has been no research on PSPTEE in preventing the proliferation of nasopharyngeal mucosal epithelial cells induced by formaldehyde through the oxidative stress mechanisms and inflammatory process. Studies have proven the effect of PSP on various cancer cells, but the carcinogenesis process of the nasopharyngeal mucosal epithelium is still limited. Hence, this study aimed to demonstrate the mechanism for preventing histopathology changes with PSPTEE due to formaldehyde exposure.METHODS: Thirty-two formaldehyde-induced Wistar rats were treated with or without 1g/kgBW/day PSPTEE for 16 weeks. Malondialdehyde (MDA) level were examined with spectrophotometry method, while tumor necrosis factor (TNF)-α and p53 with enzyme linked immunosorbent assay (ELISA) method. The histopathology appearance of the nasopharyngeal epithelium was observed with hematoxylin-eosin (HE) staining.RESULTS: MDA and TNF-α levels in control and treatment group were 22.89±2.84 μM; 9.83±0.89 μM and 84.18±11.58 ng/L; 73.92±10.59 ng/L, respectively, and they showed significant difference. Meanwhile, the level of p53 showed no significant difference. Histopathology appearance showed a significant difference. Path analysis for MDA, TNF-α and p53 levels contributed 28.7% to histopathology appearance. MDA have an effect on significant direct effect on TNF-α. TNF-α has a significant direct effect to histopathological appearance. Indirect influence MDA on histopatological appearance were smaller than the direct effect. TNF-α has the greatest influence on histopatological appearance.CONCLUSION: Formaldehyde induces the histopathology appearance in nasopharyngeal epithelium. This study also demonstrates the mechanism to prevent the nasopharynx epithelial histopathology appearance by administration of PSPTEE.KEYWORDS: PSP, MDA, TNF-α, p53, nasopharynx epithelial

show abstract

“…Papers with different topics, and 1 paper for which data related to chronic otitis media could not be extracted. Finally, eight papers were included in our meta-analysis [20][21][22][23][24][25][26][27][28][29]. The literature screening process is shown in Figure 1.…”

Section: The Results and General Characteristics Of The Included Lite...mentioning

confidence: 99%

A meta-analysis on genetic susceptibility to chronic otitis media

Cao,

Yang,

et al. 2023

Preprint

View full text Add to dashboard Cite

Chronic otitis media (COM) is known for its high prevalence (approximately 80% in children) and is thought to share common genetic risk factors, which means that there is a need to systematically study susceptibility genes associated with COM. We screened studies associated with COM genes between January 2019 and February 2023. Ten papers with 932 patients meeting the criteria were finally included, and a case-control analysis of the relationship between gene polymorphisms and COM susceptibility was performed using RevMan 5.3 software. The literature on the relationship between polymorphisms in genes related to susceptibility to chronic otitis media and chronic otitis media was tested for consistency and data merged according to different genes, and some genes were found to be polymorphic. Attitude, VEGF and Nrf2 were correlated with chronic otitis media (P < 0.05). Gene polymorphism and chronic otitis media may be related to the nationality of patients; TNF- α, IL-10, VEGF and Nrf2 may promote the formation of granulation tissue during the inflammatory phase and participate in the repair process of chronic inflammation; high expression of Nrf2 in the middle ear tissue of chronic otitis media is related to the pathogenesis of chronic otitis media.

show abstract

TNF-α and TGF-β Contributes in Recurrent Otorrhea of Active Mucosal Chronic Otitis Media

Cited by 4 publications

References 0 publications

Paneth Cell Hyperplasia and Metaplasia in Hirschsprung-associated Enterocolitis in An Aganglionosis Rat Model

Paneth Cell Hyperplasia and Metaplasia in Hirschsprung-associated Enterocolitis in An Aganglionosis Rat Model

Purple Sweet Potato Reduces Malondialdehyde and TNF-a, Increases p53, and Protects Histopathological Appearance in Formaldehyde-induced Nasopharyngeal Carcinoma Rats

A meta-analysis on genetic susceptibility to chronic otitis media

Contact Info

Product

Resources

About