TNF-alpha expression, evaluation of collagen, and TUNEL of Matricaria recutita L. extract and triamcinolone on oral ulcer in diabetic rats

Oliveira, Bruna Vasconcelos; Silva, Paulo Goberlânio de Barros; Nojosa, Jacqueline de Santiago; Brizeno, Luiz André Cavalcante; Ferreira, Jamile Magalhães; Sousa, Fabrício Bitú; Mota, Mário Rogério Lima; Alves, Ana Paula Negreiros Nunes

doi:10.1590/1678-775720150481

Cited by 50 publications

(41 citation statements)

References 28 publications

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“…However, the triamcinolone was superior to M. recutita on the 6 th day [ 33 ]. This finding supports the results found in the study that showed that the extract provided pain relief in 82% of patients with stomatitis after 5, 10, and 15 minutes; the authors concluded that the analgesic effect improved the patients' quality of life [ 21 ].…”

Section: Discussionsupporting

confidence: 89%

Effects of Matricaria Recutita (L.) in the Treatment of Oral Mucositis

Gomes

et al. 2018

The Scientific World Journal

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Objective The objective of this study was to review the effects of the Matricaria recutita (L.) in the treatment of oral mucositis. Methodology The online search was performed in the period from June 2016 to April 2018 by means of databases LILACS (Latin American and Caribbean Center on Health Sciences Information), SciELO (Scientific Electronic Library Online), and MEDLINE (Medical Literature Analysis and Retrieval System Online). The consultation was restricted to the years 1991 to 2018 with the aim of elucidating the effects of Matricaria recutita in the treatment of oral mucositis. Results The final sample consisted of 21 studies, of which 10 were developed in animals and 11 in humans, published from 1991 to 2017, with a total sample of 644 patients. The total number of patients treated with Matricaria included in 11 studies was 364, while in the control groups the total number was 280. In experimental studies, animal models used were rats and the sample size ranged between 36 and 105 animals submitted to the induction of oral mucositis, where 4 studies used an intraperitoneal injection of 5-fluorouracil, while 7 induced lesion in the mucosa. From the data collected, it should be noted that both studies with humans and with animals showed significant effects. In this way, there is strong evidence for the discussion on the therapy; however, it should be noted that more studies are developed in order to clarify the most appropriate protocol for the prevention and treatment of injuries. Conclusion According to the results found in this study, Matricaria recutita appeared to be a promising alternative for the treatment of oral mucositis. However, due to the great variability in the various types of intervention, more controlled double-blind randomized clinical studies are necessary to ensure the best protocol for treating oral mucositis.

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Section: Discussionsupporting

confidence: 89%

Effects of Matricaria Recutita (L.) in the Treatment of Oral Mucositis

Gomes

et al. 2018

The Scientific World Journal

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“…The ulcers were measured using a digital pachymeter, and all measurements were performed by the same examiner. The largest (D) and smallest (d) diameters of the lesion were recorded and the area of the ulcer was calculated as previously described in the literature (Brizeno et al, ; Lim et al, ; Oliveira et al, ), using the following formula: A = π × D/2 × d/2.…”

Section: Methodsmentioning

confidence: 99%

“…However, it is known that, when in excess, it can delay healing (Brizeno et al, ; Oliveira et al, ) and result in a greater production of free radicals that may have toxic effects on tissues (Kasuya & Tokura, ). Thus, the modulation of the release of proinflammatory cytokines is a challenging goal when the aim is to optimize healing time and to reduce pain symptoms of ulcerative lesions (Oliveira et al, ; Wagner et al, ). Several studies have investigated the development of an ideal healing agent, although there is still no widely accepted or gold standard treatment for the management of oral ulcers.…”

Section: Introductionmentioning

confidence: 99%

“…Several studies have investigated the development of an ideal healing agent, although there is still no widely accepted or gold standard treatment for the management of oral ulcers. Different strategies are often combined and involve the use of corticosteroids (Oliveira et al, ), antibiotics (Teixeira et al, ), laser therapy (Wagner et al, ), and natural compound formulations (Coelho et al, ; Lim et al, ). However, these alternatives may not have significant benefits or can even lead to unwanted side effects (Coelho et al, ; Oliveira et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Effects of cannabidiol, a Cannabis sativa constituent, on oral wound healing process in rats: Clinical and histological evaluation

Klein

Bortolli

Guimarães

et al. 2018

Phytotherapy Research

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The effects of cannabidiol (CBD), the main non-psychotropic component of Cannabis sativa, were assessed on oral wound healing in an in vivo model. Standardized ulcers were induced in 60 Wistar rats using a 5-mm biopsy punch on the midline of the ventral tongue. Animals received intraperitoneal injections of CBD at doses of 0 (control), 5, and 10 mg/kg daily. Animals were weighed daily, and wound healing was clinically and histologically evaluated after 3 and 7 days of treatment. CBD treatment did not influence the wound area of ulcerative lesions at either observation time. Conversely, microscopic findings revealed that at Day 3 postwounding, CBD-treated lesions exhibited significantly lower inflammatory scores than those in the control group. However, this difference was not observed at Day 7. Collectively, these findings indicate that CBD exert an antiinflammatory effect in early phase of wound healing process although it was not sufficient promote clinical improvement of oral traumatic ulcerative lesions.

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“…Though it may be temporary, the NGF expression almost reached the baseline level and had a rising trend in dorsal horn and DRG respectively, which could be explained by the compensatory expression of NGF. For example, the expression of tumor necrosis factor alpha (TNF-α) increases in rats with diabetes (24), and TNF-α can promote the release of NGF in astrocytes (25). In addition, the hyperalgesia was observed 1, 2, 4, and 8 weeks after diabetes induction, consistent with the time of NGF decrease in DRG.…”

Section: E558mentioning

confidence: 74%

The Different Dynamic Changes of Nerve Growth Factor in the Dorsal Horn and Dorsal Root Ganglion Leads to Hyperalgesia and Allodynia in Diabetic Neuropathic Pain

Gao

Feng

2017

Pain Phys

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Background: Diabetic peripheral neuropathy (DPN) is the most common complication of diabetes and more than half of the patients with DPN have self-reported symptoms referring to painful diabetic neuropathy (PDN). Nerve growth factor (NGF) is a key factor for the nervous system, but the role of it in the neuropathic pain of diabetic patients is unclear. Objective: This study aimed to investigate the relationship between the dynamic expression of NGF in dorsal horn and dorsal root ganglion (DRG) of diabetic rats and hyperalgesia and allodynia in diabetic neuropathic pain. It also aimed to explore the effects of exogenous mouse NGF (mNGF) on NGF expression in dorsal horn, DRG, and mechanical pain threshold. Study Design: Animal research study. Setting: Experimental research laboratory. Methods: The model of diabetes was established by a single intraperitoneal injection of streptozocin (STZ 55 mg/kg). Firstly, the rats were randomly divided into 2 groups: control group (n = 10) and diabetes group (n = 40). The diabetes group contained 4 subgroups: diabetes week 1 group (DM1, n = 10), diabetes week 2 group (DM2, n = 10), diabetes week 4 group (DM4, n = 10), and diabetes week 8 group (DM8, n = 10). Then, the other rats were randomly divided into 2 groups: control group (n = 10) and treatment group (n = 30). The treatment group contained 3 subgroups: saline group (n = 10), low dose mNGF group (mNGF1, n = 10), and high dose mNGF group (mNGF2, n = 10). Mechanical pain threshold was assessed using Von Frey hairs, before the establishment of the diabetes model and 1, 2, 4, and 8 weeks after the establishment. The NGF expression in dorsal horn and DRG was measured by western blot. Results: The mechanical pain threshold decreased one week after the establishment of the diabetes model, which continued for 8 weeks. The NGF expression in the dorsal horn was reduced 2 weeks after diabetes induction and the decreased NGF expression continued for 4 weeks. However, the NGF expression in DRG was reduced one week after diabetes induction and remained at a low level for 8 weeks. Hyperalgesia occurred when the NGF expression in the DRG decreased and further reduction in the NGF expression in the dorsal horn caused concomitant allodynia. The mechanical pain threshold was significantly elevated 2 weeks after mNGF treatment. Limitations: The course of diabetes should be much longer and there is not a precise analysis of the quantitative relation between the NGF expression in the dorsal horn/DRG and hyperalgesia/ allodynia. Conclusion: In diabetic neuropathic pain, the dynamic changes of the NGF expression in dorsal horn and DRG is involved in the development of hyperalgesia and allodynia respectively. Exogenous mNGF may relieve diabetic neuropathic pain by increasing the NGF expression in dorsal horn and DRG. Key words: Nerve growth factor, diabetic peripheral neuropathy, pain, hyperalgesia, allodynia, dorsal horn, dorsal root ganglion Pain Physician 2017; 20:E551

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TNF-alpha expression, evaluation of collagen, and TUNEL of Matricaria recutita L. extract and triamcinolone on oral ulcer in diabetic rats

Cited by 50 publications

References 28 publications

Effects of Matricaria Recutita (L.) in the Treatment of Oral Mucositis

Effects of Matricaria Recutita (L.) in the Treatment of Oral Mucositis

Effects of cannabidiol, a Cannabis sativa constituent, on oral wound healing process in rats: Clinical and histological evaluation

The Different Dynamic Changes of Nerve Growth Factor in the Dorsal Horn and Dorsal Root Ganglion Leads to Hyperalgesia and Allodynia in Diabetic Neuropathic Pain

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