Effects of cannabidiol, a Cannabis sativa constituent, on oral wound healing process in rats: Clinical and histological evaluation

Abstract

The effects of cannabidiol (CBD), the main non-psychotropic component of Cannabis sativa, were assessed on oral wound healing in an in vivo model. Standardized ulcers were induced in 60 Wistar rats using a 5-mm biopsy punch on the midline of the ventral tongue. Animals received intraperitoneal injections of CBD at doses of 0 (control), 5, and 10 mg/kg daily. Animals were weighed daily, and wound healing was clinically and histologically evaluated after 3 and 7 days of treatment. CBD treatment did not influence the wound area of ulcerative lesions at either observation time. Conversely, microscopic findings revealed that at Day 3 postwounding, CBD-treated lesions exhibited significantly lower inflammatory scores than those in the control group. However, this difference was not observed at Day 7. Collectively, these findings indicate that CBD exert an antiinflammatory effect in early phase of wound healing process although it was not sufficient promote clinical improvement of oral traumatic ulcerative lesions.

Cannabidiol on 5‐FU‐induced oral mucositis in mice

“…The effects of the administration of different concentrations of CBD were evaluated to establish a dose-response relationship, since the literature does not provide a consensus on the ideal dose for anti-oxidant and anti-inflammatory action. In previous studies, the doses tested varied from 1 to 30 mg, but none of them stood out as advantageous in relation to the others in this study (Aviello et al, 2012;Callejas et al, 2018;Cassol-Jr et al, 2010;Chen et al, 2016;Elbaz et al, 2015;Klein et al, 2018;Napimoga et al, 2009;Pan et al, 2009;Yang et al, 2014).…”

“…In Wistar male rats, CBD showed an anti-inflammatory action in the healing process in trauma-induced lesions (Klein et al, 2018) and a decrease in local bone loss and lower NF-kB expression in induced periodontitis (Napimoga et al, 2009). These results suggest that CBD can be useful in the control of inflammation caused by induced periodontal disease in rats.…”

“…Recent studies that have tested the use of CBD for other diseases of inflammatory origin discuss the ability of the product to modulate the immune response by decreasing the production of pro-inflammatory cytokines such as TNF-alpha, Nf-Kb, and interleukins, and the migration of neutrophils to the periphery of the damaged tissues, which would reduce the tissue inflammatory reaction (Klein et al, 2018;Napimoga et al, 2009;Rajan et al, 2016;Rajesh et al, 2010).…”

“…intensity of inflammatory response was chosen to determine the score, according to the criteria below (Figueiredo, Pesce, Gioso, & Figueiredo, 2001;de Freitas Cuba, Braga Filho, Cherubini, Salum, & Fig ueiredo, 2016;Klein et al, 2018 and anti-oxidant reduced glutathione (GSH) were determined using a Cary 100 spectrophotometer.…”

Trends in use, pharmacology, and clinical applications of emerging herbal nutraceuticals

“…For instance, CBD is effective in models of neuropathic pain induced by chemotherapy(Harris, Sufka, Gul, & ElSohly, 2016;Ward et al, 2014), diabetes(Jesus et al, 2019) nerve chronic constriction(Belardo et al, 2019) and injury(De Gregorio, McLaughlin, et al, 2019). Similarly, CBD may exert analgesic and/or anti-inflammatory effects in animal models of arthritis(Hammell et al, 2016), airways inflammation(Vuolo et al, 2019), allergic contact dermatitis(Petrosino et al, 2018), oral wounds(Klein et al, 2018) and colitis(Pagano et al, 2016). In arthritis animal models, CBD provided pain relief and reduced inflammatory via a combination of immunosuppressive and anti-inflammatory effects(Lowin et al, 2019).…”