TMT-based quantitative proteomic analysis reveals defense mechanism of wheat against the crown rot pathogen Fusarium pseudograminearum

Qiao, Fangfang; Yang, Xin; Xu, Fengdan; Huang, Yuan; Zhang, Jiemei; Miao, Song; Zhou, Sumei; Zhang, Meng; He, Dexian

doi:10.1186/s12870-021-02853-6

Cited by 13 publications

(8 citation statements)

References 73 publications

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“…The differentially expressed genes of Arabidopsis infected by Verticillium dahliae are mainly enriched in metabolic pathways, such as plant–pathogen interaction and plant hormone signal transduction [ 53 ]. After the wheat was infected by Fusarium pseudograminearum , phenylpropane biosynthesis, glutathione metabolism, and plant hormone signal transduction occurred in defense [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

Functional Identification of Arthrinium phaeospermum Effectors Related to Bambusa pervariabilis × Dendrocalamopsis grandis Shoot Blight

et al. 2022

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The shoot blight of Bambusa pervariabilis × Dendrocalamopsis grandis caused by Arthrinium phaeospermum made bamboo die in a large area, resulting in serious ecological and economic losses. Dual RNA-seq was used to sequence and analyze the transcriptome data of A. phaeospermum and B. pervariabilis × D. grandis in the four periods after the pathogen infected the host and to screen the candidate effectors of the pathogen related to the infection. After the identification of the effectors by the tobacco transient expression system, the functions of these effectors were verified by gene knockout. Fifty-three differentially expressed candidate effectors were obtained by differential gene expression analysis and effector prediction. Among them, the effectors ApCE12 and ApCE22 can cause programmed cell death in tobacco. The disease index of B. pervariabilis × D. grandis inoculated with mutant ΔApCE12 and mutant ΔApCE22 strains were 52.5% and 47.5%, respectively, which was significantly lower than that of the wild-type strains (80%), the ApCE12 complementary strain (77.5%), and the ApCE22 complementary strain (75%). The tolerance of the mutant ΔApCE12 and mutant ΔApCE22 strains to H2O2 and NaCl stress was significantly lower than that of the wild-type strain and the ApCE12 complementary and ApCE22 complementary strains, but there was no difference in their tolerance to Congo red. Therefore, this study shows that the effectors ApCE12 and ApCE22 play an important role in A. phaeospermum virulence and response to H2O2 and NaCl stress.

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Section: Discussionmentioning

confidence: 99%

Functional Identification of Arthrinium phaeospermum Effectors Related to Bambusa pervariabilis × Dendrocalamopsis grandis Shoot Blight

et al. 2022

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“…Molecular mechanisms of the interaction between the Fusarium pseudograminearum WZ-8A and two wheat cultivars were explored [ 171 ]. Crown rot pathogen F. pseudograminearum was inoculated into UC1110, disease-susceptible and PI61750, disease-resistant wheat cultivars.…”

Section: Proteomics Approachesmentioning

confidence: 99%

Wheat Omics: Advancements and Opportunities

Sehgal

Dhakate

Ambreen

et al. 2023

Plants

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Plant omics, which includes genomics, transcriptomics, metabolomics and proteomics, has played a remarkable role in the discovery of new genes and biomolecules that can be deployed for crop improvement. In wheat, great insights have been gleaned from the utilization of diverse omics approaches for both qualitative and quantitative traits. Especially, a combination of omics approaches has led to significant advances in gene discovery and pathway investigations and in deciphering the essential components of stress responses and yields. Recently, a Wheat Omics database has been developed for wheat which could be used by scientists for further accelerating functional genomics studies. In this review, we have discussed various omics technologies and platforms that have been used in wheat to enhance the understanding of the stress biology of the crop and the molecular mechanisms underlying stress tolerance.

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“…For these reasons, FCR is a growing threat to wheat cultivation in wheat-maize rotation regions in North China. Based on previous omics studies, wheat resistance to FCR was associated with transcriptional activations of transcription factor, cellular transport and detoxification genes, as well as protein accumulations in photosynthesis, secondary metabolite biosynthesis, phenylpropanoid biosynthesis, and glutathione metabolism ( Powell et al, 2017 ; Qiao et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Genetics of Resistance to Common Root Rot (Spot Blotch), Fusarium Crown Rot, and Sharp Eyespot in Wheat

Zhao

et al. 2021

Front. Genet.

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Due to soil changes, high density planting, and the use of straw-returning methods, wheat common root rot (spot blotch), Fusarium crown rot (FCR), and sharp eyespot (sheath blight) have become severe threats to global wheat production. Only a few wheat genotypes show moderate resistance to these root and crown rot fungal diseases, and the genetic determinants of wheat resistance to these devastating diseases are poorly understood. This review summarizes recent results of genetic studies of wheat resistance to common root rot, Fusarium crown rot, and sharp eyespot. Wheat germplasm with relatively higher resistance are highlighted and genetic loci controlling the resistance to each disease are summarized.

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TMT-based quantitative proteomic analysis reveals defense mechanism of wheat against the crown rot pathogen Fusarium pseudograminearum

Cited by 13 publications

References 73 publications

Functional Identification of Arthrinium phaeospermum Effectors Related to Bambusa pervariabilis × Dendrocalamopsis grandis Shoot Blight

Functional Identification of Arthrinium phaeospermum Effectors Related to Bambusa pervariabilis × Dendrocalamopsis grandis Shoot Blight

Wheat Omics: Advancements and Opportunities

Genetics of Resistance to Common Root Rot (Spot Blotch), Fusarium Crown Rot, and Sharp Eyespot in Wheat

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