Titanium discs coated with 3,4-dihydroxy-<scp>l</scp>-phenylalanine promote osteogenic differentiation of human bone mesenchymal stem cells <i>in vitro</i>

Ma, Ting; Ge, Xi‐Yuan; Hao, Ke-Yi; Jiang, Xi; Zheng, Yinggan; Lin, Ye

doi:10.1039/c8ra09952a

Cited by 7 publications

(4 citation statements)

References 56 publications

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“…The surfaces of immobilized exosomes and the combination of immobilized and suspended exosomes increased the surface coverage of cells as compared to the Ti control surface. Likewise, bioinspired material 3,4-dihydroxy-L-phenylalanine (DOPA)coated Ti surface exhibited enhancement of cellular adhesion and spreading compared to the uncoated control [49]. It was impressive that simple DOPA coating signi cantly enhanced ALP activity of MSCs compared to control cells at day 7 of the investigation.…”

Section: Discussionmentioning

confidence: 99%

Enhanced osseointegration of dental implants with reduced graphene oxide coating

Shin

Bae

Lee

et al. 2022

Preprint

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Background: The implants of pure titanium (Ti) and its alloys can lead to implant failure because of their poor interaction with bone-associated cells during bone regeneration. Surface modification over implants has achieved successful implants for enhanced osseointegration.Methods: Herein, we prepared sandblasted, large-grit, and acid-etched (SLA) Ti (ST) implants with different surface modifications [i.e., reduced graphene oxide (rGO) and recombinant human bone morphogenetic protein-2 (rhBMP-2)] and investigated their dental tissue regenerating ability in animal models. We performed comparative studies in surface property, in vitro cellular behaviors, and in vivo osseointegration activity among different groups, including ST (control), rhBMP-2-immobilized ST (BI-ST), rhBMP-2-treated ST (BT-ST), and rGO-coated ST (R-ST).Results: Spectroscopic, diffractometric, and microscopic analyses confirmed that rGO was coated well around the surfaces of Ti discs (for cell study) and implant fixtures (for animal study). Furthermore, in vitro and in vivo studies revealed that the R-ST group showed significantly better effects in cell attachment and proliferation, alkaline phosphatase activity, matrix mineralization, and osseointegration than the control (ST), BI-ST, and BT-ST groups.Conclusion: Hence, we suggest that the rGO-coated Ti can be a promising candidate for the application to dental or even orthopedic implants due to its ability to accelerate the healing rate with the high potential of osseointegration.

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Section: Discussionmentioning

confidence: 99%

Enhanced osseointegration of dental implants with reduced graphene oxide coating

Shin

Bae

Lee

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…The surfaces of immobilized exosomes and the combination of immobilized and suspended exosomes increased the surface coverage of cells as compared to the Ti control surface. Likewise, bioinspired material 3,4-dihydroxy-L-phenylalanine (DOPA)-coated Ti surface exhibited enhancement of cellular adhesion and spreading compared to the uncoated control [ 50 ]. It was impressive that simple DOPA coating significantly enhanced the ALP activity of MSCs compared to control cells at day 7 of the investigation.…”

Section: Discussionmentioning

confidence: 99%

Enhanced osseointegration of dental implants with reduced graphene oxide coating

et al. 2022

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Background The implants of pure titanium (Ti) and its alloys can lead to implant failure because of their poor interaction with bone-associated cells during bone regeneration. Surface modification over implants has achieved successful implants for enhanced osseointegration. Herein, we report a robust strategy to implement bioactive surface modification for implant interface enabled by the combinatorial system of reduced graphene oxide (rGO)-coated sandblasted, large-grit, and acid-etched (SLA) Ti to impart benefits to the implant. Methods We prepared SLA Ti (ST) implants with different surface modifications [i.e., rGO and recombinant human bone morphogenetic protein-2 (rhBMP-2)] and investigated their dental tissue regenerating ability in animal models. We performed comparative studies in surface property, in vitro cellular behaviors, and in vivo osseointegration activity among different groups, including ST (control), rhBMP-2-immobilized ST (BI-ST), rhBMP-2-treated ST (BT-ST), and rGO-coated ST (R-ST). Results Spectroscopic, diffractometric, and microscopic analyses confirmed that rGO was coated well around the surfaces of Ti discs (for cell study) and implant fixtures (for animal study). Furthermore, in vitro and in vivo studies revealed that the R-ST group showed significantly better effects in cell attachment and proliferation, alkaline phosphatase activity, matrix mineralization, expression of osteogenesis-related genes and protein, and osseointegration than the control (ST), BI-ST, and BT-ST groups. Conclusion Hence, we suggest that the rGO-coated Ti can be a promising candidate for the application to dental or even orthopedic implants due to its ability to accelerate the healing rate with the high potential of osseointegration.

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“…Copyright 2021, Elsevier. (Wang H. et al, 2019;Ma et al, 2019;Yin et al, 2019). Meanwhile, the hydrophilicity is also improved (Figure 4B), which has been proved able to enhance cell adhesion, proliferation and differentiation (Wang P. et al, 2021).…”

Section: Property and Function Of Pda-modified Surfacesmentioning

confidence: 95%

“…After PDA modification, there are distinct differences in surface physiochemical property ( Figure 4 ). Under scanning electron microscope (SEM) and atomic force microscope (AFM), the granular PDA aggregates can be observed formed and spread uniformly ( Figures 4A,D ), with increased surface roughness ( Wang H. et al, 2019 ; Ma et al, 2019 ; Yin et al, 2019 ). Meanwhile, the hydrophilicity is also improved ( Figure 4B ), which has been proved able to enhance cell adhesion, proliferation and differentiation ( Wang P. et al, 2021 ).…”

Section: Formation and Function Of Pda Coatingmentioning

confidence: 99%

Mussel-Inspired Polydopamine-Based Multilayered Coatings for Enhanced Bone Formation

Zhao

Lin

et al. 2022

Front. Bioeng. Biotechnol.

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Repairing bone defects remains a challenge in clinical practice and the application of artificial scaffolds can enhance local bone formation, but the function of unmodified scaffolds is limited. Considering different application scenarios, the scaffolds should be multifunctionalized to meet specific demands. Inspired by the superior adhesive property of mussels, polydopamine (PDA) has attracted extensive attention due to its universal capacity to assemble on all biomaterials and promote further adsorption of multiple external components to form PDA-based multilayered coatings with multifunctional property, which can induce synergistic enhancement of new bone formation, such as immunomodulation, angiogenesis, antibiosis and antitumor property. This review will summarize mussel-inspired PDA-based multilayered coatings for enhanced bone formation, including formation mechanism and biofunction of PDA coating, as well as different functional components. The synergistic enhancement of multiple functions for better bone formation will also be discussed. This review will inspire the design and fabrication of PDA-based multilayered coatings for different application scenarios and promote deeper understanding of their effect on bone formation, but more efforts should be made to achieve clinical translation. On this basis, we present a critical conclusion, and forecast the prospects of PDA-based multilayered coatings for bone regeneration.

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Titanium discs coated with 3,4-dihydroxy-l-phenylalanine promote osteogenic differentiation of human bone mesenchymal stem cells in vitro

Abstract: Titanium discs with simple 3,4-dihydroxy-l-phenylalanine coating enhanced BM-MSC adhesion, spreading, proliferation and differentiation, and upregulated expression of genes involved in focal adhesion in vitro.

Cited by 7 publications

References 56 publications

Enhanced osseointegration of dental implants with reduced graphene oxide coating

Enhanced osseointegration of dental implants with reduced graphene oxide coating

Enhanced osseointegration of dental implants with reduced graphene oxide coating

Mussel-Inspired Polydopamine-Based Multilayered Coatings for Enhanced Bone Formation

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