Titanium Delivery of Osteoblastic Cell Sheets: An &lt;i&gt;In Vitro&lt;/i&gt; Study

Kanuru, Rajita Kodali; Sugita, Yoshihiko; Ikeda, Takayuki; Shinwari, Hasnain; Ishijima, Manabu; Honda, Yuma; Maeda, Hideaki; Ogawa, Takahiro

doi:10.2485/jhtb.27.43

Cited by 5 publications

(4 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite advances in scaffold development and the identification of new growth factors and their genetic manipulation [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 ], delivering sufficient numbers of cells and maintaining them in areas of interest, regardless of whether for ex vivo tissue engineering or in situ regeneration, has been challenging [ 1 , 12 , 15 , 16 , 17 , 18 , 19 , 20 ]. The effective delivery of stem cells or osteogenic cells derived from bone marrow or other source organs has been investigated in the context of tissue engineering and regeneration [ 12 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 ]. Various methods to improve cellular delivery have also been examined, including cell sheet technology, injectable vehicles, carrier molecules, and 3-dimensional (3D) cell printing [ 2 , 27 , …”

Section: Introductionmentioning

confidence: 99%

“…The effective delivery of stem cells or osteogenic cells derived from bone marrow or other source organs has been investigated in the context of tissue engineering and regeneration [ 12 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 ]. Various methods to improve cellular delivery have also been examined, including cell sheet technology, injectable vehicles, carrier molecules, and 3-dimensional (3D) cell printing [ 2 , 27 , 29 , 46 , 47 , 48 , 49 , 50 , 51 , 52 ]. Despite some progress, cell-based repair and regeneration of tissues, particularly fractured and diseased bone remains far from being a standard clinical modality, with the approach hampered by poor clinical viability, high costs, and suboptimal biological efficacy [ 37 , 38 , 47 , 53 , 54 , 55 , 56 , 57 , 58 , 59 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A Novel Cell Delivery System Exploiting Synergy between Fresh Titanium and Fibronectin

Hirota

Hori

Sugita

et al. 2022

Cells

Self Cite

View full text Add to dashboard Cite

Delivering and retaining cells in areas of interest is an ongoing challenge in tissue engineering. Here we introduce a novel approach to fabricate osteoblast-loaded titanium suitable for cell delivery for bone integration, regeneration, and engineering. We hypothesized that titanium age influences the efficiency of protein adsorption and cell loading onto titanium surfaces. Fresh (newly machined) and 1-month-old (aged) commercial grade 4 titanium disks were prepared. Fresh titanium surfaces were hydrophilic, whereas aged surfaces were hydrophobic. Twice the amount of type 1 collagen and fibronectin adsorbed to fresh titanium surfaces than aged titanium surfaces after a short incubation period of three hours, and 2.5-times more fibronectin than collagen adsorbed regardless of titanium age. Rat bone marrow-derived osteoblasts were incubated on protein-adsorbed titanium surfaces for three hours, and osteoblast loading was most efficient on fresh titanium adsorbed with fibronectin. The number of osteoblasts loaded using this synergy between fresh titanium and fibronectin was nine times greater than that on aged titanium with no protein adsorption. The loaded cells were confirmed to be firmly attached and functional. The number of loaded cells was strongly correlated with the amount of protein adsorbed regardless of the protein type, with fibronectin simply more efficiently adsorbed on titanium surfaces than collagen. The role of surface hydrophilicity of fresh titanium surfaces in increasing protein adsorption or cell loading was unclear. The hydrophilicity of protein-adsorbed titanium increased with the amount of protein but was not the primary determinant of cell loading. In conclusion, the osteoblast loading efficiency was dependent on the age of the titanium and the amount of protein adsorption. In addition, the efficiency of protein adsorption was specific to the protein, with fibronectin being much more efficient than collagen. This is a novel strategy to effectively deliver osteoblasts ex vivo and in vivo using titanium as a vehicle.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Novel Cell Delivery System Exploiting Synergy between Fresh Titanium and Fibronectin

Hirota

Hori

Sugita

et al. 2022

Cells

Self Cite

View full text Add to dashboard Cite

show abstract

“…Titanium is an osteoconductive material routinely used in dentistry and orthopedics as an implant material in repairs and reconstruction of teeth, joints, and bony sites [1][2][3][4][5][6][7][8][9]. The integration of titanium into bone is known as osseointegration, and its strength depends on the surface texture of the titanium [6,8,[10][11][12][13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Ultraviolet Light Treatment of Titanium Microfiber Scaffolds Enhances Osteoblast Recruitment and Osteoconductivity in a Vertical Bone Augmentation Model: 3D UV Photofunctionalization

Kitajima

Hirota

Komatsu

et al. 2022

Cells

Self Cite

View full text Add to dashboard Cite

Vertical bone augmentation to create host bone prior to implant placement is one of the most challenging regenerative procedures. The objective of this study is to evaluate the capacity of a UV-photofunctionalized titanium microfiber scaffold to recruit osteoblasts, generate intra-scaffold bone, and integrate with host bone in a vertical augmentation model with unidirectional, limited blood supply. Scaffolds were fabricated by molding and sintering grade 1 commercially pure titanium microfibers (20 μm diameter) and treated with UVC light (200–280 nm wavelength) emitted from a low-pressure mercury lamp for 20 min immediately before experiments. The scaffolds had an even and dense fiber network with 87% porosity and 20–50 mm inter-fiber distance. Surface carbon reduced from 30% on untreated scaffold to 10% after UV treatment, which corresponded to hydro-repellent to superhydrophilic conversion. Vertical infiltration testing revealed that UV-treated scaffolds absorbed 4-, 14-, and 15-times more blood, water, and glycerol than untreated scaffolds, respectively. In vitro, four-times more osteoblasts attached to UV-treated scaffolds than untreated scaffolds three hours after seeding. On day 2, there were 70% more osteoblasts on UV-treated scaffolds. Fluorescent microscopy visualized confluent osteoblasts on UV-treated microfibers two days after seeding but sparse and separated cells on untreated microfibers. Alkaline phosphatase activity and osteocalcin gene expression were significantly greater in osteoblasts grown on UV-treated microfiber scaffolds. In an in vivo model of vertical augmentation on rat femoral cortical bone, the interfacial strength between innate cortical bone and UV-treated microfiber scaffold after two weeks of healing was double that observed between bone and untreated scaffold. Morphological and chemical analysis confirmed seamless integration of the innate cortical and regenerated bone within microfiber networks for UV-treated scaffolds. These results indicate synergy between titanium microfiber scaffolds and UV photofunctionalization to provide a novel and effective strategy for vertical bone augmentation.

show abstract

“…The primary requirement for successful dental implant treatments is sufficient boneimplant integration [1][2][3][4][5][6][7][8][9][10][11]. This has led to a significant body of research on improving the integration between the surface of titanium implants and bone [2,4,5,.…”

Section: Introductionmentioning

confidence: 99%

Ultraviolet Treatment of Titanium to Enhance Adhesion and Retention of Oral Mucosa Connective Tissue and Fibroblasts

Ikeda

Ueno

Saruta

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

Peri-implantitis is an unsolved but critical problem with dental implants. It is postulated that creating a seal of gingival soft tissue around the implant neck is key to preventing peri-implantitis. The objective of this study was to determine the effect of UV surface treatment of titanium disks on the adhesion strength and retention time of oral connective tissues as well as on the adherence of mucosal fibroblasts. Titanium disks with a smooth machined surface were prepared and treated with UV light for 15 min. Keratinized mucosal tissue sections (3 × 3 mm) from rat palates were incubated for 24 h on the titanium disks. The adhered tissue sections were then mechanically detached by agitating the culture dishes. The tissue sections remained adherent for significantly longer (15.5 h) on the UV-treated disks than on the untreated control disks (7.5 h). A total of 94% of the tissue sections were adherent for 5 h or longer on the UV-treated disks, whereas only 50% of the sections remained on the control disks for 5 h. The adhesion strength of the tissue sections to the titanium disks, as measured by tensile testing, was six times greater after UV treatment. In the culture studies, mucosal fibroblasts extracted from rat palates were attached to titanium disks by incubating for 24, 48, or 96 h. The number of attached cells was consistently 15–30% greater on the UV-treated disks than on the control disks. The cells were then subjected to mechanical or chemical (trypsinization) detachment. After mechanical detachment, the residual cell rates on the UV-treated surfaces after 24 and 48 h of incubation were 35% and 25% higher, respectively, than those on the control surfaces. The remaining rate after chemical detachment was 74% on the control surface and 88% on the UV-treated surface for the cells cultured for 48 h. These trends were also confirmed in mouse embryonic fibroblasts, with an intense expression of vinculin, a focal adhesion protein, on the UV-treated disks even after detachment. The UV-treated titanium was superhydrophilic, whereas the control titanium was hydrophobic. X-ray photoelectron spectroscopy (XPS) chemical analysis revealed that the amount of carbon at the surface was significantly reduced after UV treatment, while the amount of TiOH molecules was increased. These ex vivo and in vitro results indicate that the UV treatment of titanium increases the adhesion and retention of oral mucosa connective tissue as a result of increased resistance of constituent fibroblasts against exogenous detachment, both mechanically and chemically, as well as UV-induced physicochemical changes of the titanium surface.

show abstract

Titanium Delivery of Osteoblastic Cell Sheets: An <i>In Vitro</i> Study

Cited by 5 publications

References 29 publications

A Novel Cell Delivery System Exploiting Synergy between Fresh Titanium and Fibronectin

A Novel Cell Delivery System Exploiting Synergy between Fresh Titanium and Fibronectin

Ultraviolet Light Treatment of Titanium Microfiber Scaffolds Enhances Osteoblast Recruitment and Osteoconductivity in a Vertical Bone Augmentation Model: 3D UV Photofunctionalization

Ultraviolet Treatment of Titanium to Enhance Adhesion and Retention of Oral Mucosa Connective Tissue and Fibroblasts

Contact Info

Product

Resources

About