A Novel Cell Delivery System Exploiting Synergy between Fresh Titanium and Fibronectin

Hirota, Makoto; Hori, Norio; Sugita, Yoshihiko; Ikeda, Takayuki; Park, Won-Hee; Saruta, Juri; Ogawa, Takahiro

doi:10.3390/cells11142158

Cited by 6 publications

(4 citation statements)

References 138 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, such surface texture-induced microenvironments with stagnated and concentrated blood and proteins may explain the successful, contact osteogenesis or osseointegration. The effects of hydrophilic titanium surfaces on the recruitment of blood, proteins, and cells have been reported extensively [ 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 ]. The present study has revealed that a similar effect can occur by surface texturing.…”

Section: Discussionmentioning

confidence: 99%

The Effects of a Biomimetic Hybrid Meso- and Nano-Scale Surface Topography on Blood and Protein Recruitment in a Computational Fluid Dynamics Implant Model

Kitajima,

Hirota,

Osawa

et al. 2023

Biomimetics

Self Cite

View full text Add to dashboard Cite

The mechanisms underlying bone-implant integration, or osseointegration, are still incompletely understood, in particular how blood and proteins are recruited to implant surfaces. The objective of this study was to visualize and quantify the flow of blood and the model protein fibrinogen using a computational fluid dynamics (CFD) implant model. Implants with screws were designed with three different surface topographies: (1) amorphous, (2) nano-trabecular, and (3) hybrid meso-spikes and nano-trabeculae. The implant with nano-topography recruited more blood and fibrinogen to the implant interface than the amorphous implant. Implants with hybrid topography further increased recruitment, with particularly efficient recruitment from the thread area to the interface. Blood movement significantly slowed at the implant interface compared with the thread area for all implants. The blood velocity at the interface was 3- and 4-fold lower for the hybrid topography compared with the nano-topography and amorphous surfaces, respectively. Thus, this study for the first time provides insights into how different implant surfaces regulate blood dynamics and the potential advantages of surface texturization in blood and protein recruitment and retention. In particular, co-texturization with a hybrid meso- and nano-topography created the most favorable microenvironment. The established CFD model is simple, low-cost, and expected to be useful for a wide range of studies designing and optimizing implants at the macro and micro levels.

show abstract

Section: Discussionmentioning

confidence: 99%

The Effects of a Biomimetic Hybrid Meso- and Nano-Scale Surface Topography on Blood and Protein Recruitment in a Computational Fluid Dynamics Implant Model

Kitajima,

Hirota,

Osawa

et al. 2023

Biomimetics

Self Cite

View full text Add to dashboard Cite

show abstract

“…Here, we report that UV treatment enhances osteoblast behavior and osteoconductivity of titanium microfibers and their scaffolds in a vertical augmentation model with very limited blood and cellular supply from around a screw. Titanium surfaces lose osteoconductivity over time due to the progressive accumulation of hydrocarbons on their surfaces, which is also known as "biological aging" of titanium [62,94,97,98,116]. This accumulation of hydrocarbons can be cleaned by UV treatment [10,82,96,[120][121][122].…”

Section: Discussionmentioning

confidence: 99%

“…The number of osteoblasts present on the scaffolds was evaluated after 3 h and 24 h of culture using a WST-1-based colorimetric assay (WST-1, Roche Applied Science, Mannheim, Germany). WST-1 assay is based on the metabolic activity of cells and used for a wide range of quantification that includes cell viability, attachment, and proliferation, based on the assumption that the number of cells is correlated with the amount of their metabolic activity [54,[114][115][116][117]. Scaffolds in tissue culture wells were incubated at 37 • C for 1 h with 100 µL of WST-1 reagent.…”

Section: Number Of Attached and Propagated Cells Cell Attachment Assa...mentioning

confidence: 99%

Ultraviolet Light Treatment of Titanium Microfiber Scaffolds Enhances Osteoblast Recruitment and Osteoconductivity in a Vertical Bone Augmentation Model: 3D UV Photofunctionalization

Kitajima

Hirota

Komatsu

et al. 2022

Cells

Self Cite

View full text Add to dashboard Cite

Vertical bone augmentation to create host bone prior to implant placement is one of the most challenging regenerative procedures. The objective of this study is to evaluate the capacity of a UV-photofunctionalized titanium microfiber scaffold to recruit osteoblasts, generate intra-scaffold bone, and integrate with host bone in a vertical augmentation model with unidirectional, limited blood supply. Scaffolds were fabricated by molding and sintering grade 1 commercially pure titanium microfibers (20 μm diameter) and treated with UVC light (200–280 nm wavelength) emitted from a low-pressure mercury lamp for 20 min immediately before experiments. The scaffolds had an even and dense fiber network with 87% porosity and 20–50 mm inter-fiber distance. Surface carbon reduced from 30% on untreated scaffold to 10% after UV treatment, which corresponded to hydro-repellent to superhydrophilic conversion. Vertical infiltration testing revealed that UV-treated scaffolds absorbed 4-, 14-, and 15-times more blood, water, and glycerol than untreated scaffolds, respectively. In vitro, four-times more osteoblasts attached to UV-treated scaffolds than untreated scaffolds three hours after seeding. On day 2, there were 70% more osteoblasts on UV-treated scaffolds. Fluorescent microscopy visualized confluent osteoblasts on UV-treated microfibers two days after seeding but sparse and separated cells on untreated microfibers. Alkaline phosphatase activity and osteocalcin gene expression were significantly greater in osteoblasts grown on UV-treated microfiber scaffolds. In an in vivo model of vertical augmentation on rat femoral cortical bone, the interfacial strength between innate cortical bone and UV-treated microfiber scaffold after two weeks of healing was double that observed between bone and untreated scaffold. Morphological and chemical analysis confirmed seamless integration of the innate cortical and regenerated bone within microfiber networks for UV-treated scaffolds. These results indicate synergy between titanium microfiber scaffolds and UV photofunctionalization to provide a novel and effective strategy for vertical bone augmentation.

show abstract

“…This time-dependent degradation of the surface properties and bioactivity of titanium is regarded as a form of “biological aging” [ 18 , 25 , 29 , 30 , 31 , 32 ]. UV photofunctionalization can reverse the biological aging of titanium and hence improve osteoconductivity [ 26 , 40 , 43 , 61 , 63 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 ].…”

Section: Introductionmentioning

confidence: 99%

Decomposing Organic Molecules on Titanium with Vacuum Ultraviolet Light for Effective and Rapid Photofunctionalization

Suzumura

Matsuura

Komatsu

et al. 2022

JFB

Self Cite

View full text Add to dashboard Cite

Ultraviolet (UV) photofunctionalization counteracts the biological aging of titanium to increase the bioactivity and osseointegration of titanium implants. However, UV photofunctionalization currently requires long treatment times of between 12 min and 48 h, precluding routine clinical use. Here, we tested the ability of a novel, xenon excimer lamp emitting 172 nm vacuum UV (VUV) to decompose organic molecules coated on titanium as a surrogate of photofunctionalization. Methylene blue as a model organic molecule was coated on grade 4 commercially pure titanium and treated with four UV light sources: (i) ultraviolet C (UVC), (ii) high-energy UVC (HUVC), (iii) proprietary UV (PUV), and (iv) VUV. After one minute of treatment, VUV decomposed 57% of methylene blue compared with 2%, 36%, and 42% for UVC, HUVC, and PUV, respectively. UV dose-dependency testing revealed maximal methylene blue decomposition with VUV within one minute. Equivalent decomposition was observed on grade 5 titanium alloy specimens, and placing titanium specimens in quartz ampoules did not compromise efficacy. Methylene blue was decomposed even on polymethyl methacrylate acrylic specimens at 20–25% lower efficiency than on titanium specimens, indicating a relatively small contribution of titanium dioxide-mediated photocatalytic decomposition to the total decomposition. Load-testing revealed that VUV maintained high efficacy of methylene blue decomposition regardless of the coating density, whereas other UV light sources showed low efficacy with thin coatings and plateauing efficacy with thicker coatings. This study provides foundational data on rapid and efficient VUV-mediated organic decomposition on titanium. In synergy with quartz ampoules used as containers, VUV has the potential to overcome current technical challenges hampering the clinical application of UV photofunctionalization.

show abstract

A Novel Cell Delivery System Exploiting Synergy between Fresh Titanium and Fibronectin

Cited by 6 publications

References 138 publications

The Effects of a Biomimetic Hybrid Meso- and Nano-Scale Surface Topography on Blood and Protein Recruitment in a Computational Fluid Dynamics Implant Model

The Effects of a Biomimetic Hybrid Meso- and Nano-Scale Surface Topography on Blood and Protein Recruitment in a Computational Fluid Dynamics Implant Model

Ultraviolet Light Treatment of Titanium Microfiber Scaffolds Enhances Osteoblast Recruitment and Osteoconductivity in a Vertical Bone Augmentation Model: 3D UV Photofunctionalization

Decomposing Organic Molecules on Titanium with Vacuum Ultraviolet Light for Effective and Rapid Photofunctionalization

Contact Info

Product

Resources

About