Tissue Engineering Therapy for Unhealed Diabetic Wound Using Mononuclear Stem Cells, Plasma Rich Platelets and Collagen

Abstract

Diabetic wound is the most dreadful complication of Diabetes Mellitus (DM) in musculoskeletal system of human being. Diabetic wound healing is considerably challenging due to the poor ability of tissue regeneration in diabetic patients. Here we reported a case study of tissue engineering therapy on unhealed wound in the back of young female DM patient. We decided to not do any surgery treatment. We did standard wound therapy with combination tissue engineering therapy. The tissue engineering treatment will be using Peripheral Blood Mononuclear Stem Cells (PBMNCs), Cord Blood Mononuclear Stem Cells (CBMNCs), Collagen, and Plasma Ri ch Platelets (PRP). To develop a PRP preparation, blood must first be drawn from patient and put it in PRP tube. The platelets are separated from other blood cells and enriched by centrifugation. CBMNCs were isolated from human placenta blood (donor) which is collected during delivery process, the PBMNCs were isolated from patient's blood and they were extracted with Ficoll gradient. PRP and MNCs was applied topically and subcutaneously to the wound. We gave collagen every week, PBMNCs at week 4, CBMNCs at week 6 and PRP at week 8. We did tissue engineering therapy for 8 weeks and the result showed perfect wound healing without complication. In conclusion tissue engineering therapy using MNCs stem cell, PRP and collagen could treat successfully unhealed diabetic wound.

Emerging Treatment Strategies for Diabetes Mellitus and Associated Complications: An Update

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Safety evaluation of human peripheral blood mononuclear cells in naive rats:a chronic toxicitystudy

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