Tissue Engineering of Vascular Grafts: Human Cell Seeding of Decellularised Porcine Matrix

Abstract

Cellular components can be extracted from native porcine blood vessels. Vascular grafts can be generated in vitro of animal acellular matrix and human cells.

Pericardial Processing: Challenges, Outcomes and Future Prospects

“…Physical methods can either rely on snap freezing (Jackson et al, 1988;Roberts et al, 1991), mechanical force (Freytes et al, 2004) or mechanical agitation (Schenke-Layland et al, 2003), whereas enzymatic protocols employ nucleases, calcium chelating agents or protease digestion (Teebken et al, 2000;Bader et al, 1998;McFetridge et al, 2004;Gamba et al, 2002). Regarding physical decellularization processes, sonication, based on the use of ultrasounds to disrupt the cell membrane, has been investigated.…”

Reendothelialization of Human Heart Valve Neoscaffolds Using Umbilical Cord-Derived Endothelial Cells

“…This influences the growth, orientation, and phenotypic remodeling of the endothelial cells. 32- 35 It has been shown that a rapid increase in bioreactor flow causes significant damage of the reseeded endothelium and complete cell wash-off from cusps. 12 This is especially common near the valve leaflet, where turbulent flow is present.…”

Heart valve bioprothesis; effect of different acellularizations methods on the biomechanical and morphological properties of porcine aortic and pulmonary valve

“…The incubation in a Trypsin/EDTA solution is one of the most commonly used acellularization methods. Incubation in this solution allows to effectively remove the cells without visible changes in the extracellular matrix structure [11][12][13]. In our experience, the cell removal from the matrix is not completely effective after a 48 h incubation in a Trypsin/EDTA solution, and the rest of the cells or cell debris can cause degenerative changes within the tissue after a potential valve implantation.…”