Time-resolved measurements of luminescence

Collier, Bradley B.; McShane, Michael J.

doi:10.1016/j.jlumin.2013.06.034

Cited by 40 publications

(39 citation statements)

References 106 publications

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“…Maximum likelihood analysis [46] utilizes the integration of the intensity within several windows of time to determine the lifetime of the fluorophore. An iterative solver is used to estimate the lifetimes using the measurement data.…”

Section: Maximum Likelihood Analysismentioning

confidence: 99%

FLIM-FRET for Cancer Applications

Rajoria

Zhao

Intes

et al. 2015

CMI

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Optical imaging assays, especially fluorescence molecular assays, are minimally invasive if not completely noninvasive, and thus an ideal technique to be applied to live specimens. These fluorescence imaging assays are a powerful tool in biomedical sciences as they allow the study of a wide range of molecular and physiological events occurring in biological systems. Furthermore, optical imaging assays bridge the gap between the in vitro cell-based analysis of subcellular processes and in vivo study of disease mechanisms in small animal models. In particular, the application of Förster resonance energy transfer (FRET) and fluorescence lifetime imaging (FLIM), well-known techniques widely used in microscopy, to the optical imaging assay toolbox, will have a significant impact in the molecular study of protein-protein interactions during cancer progression. This review article describes the application of FLIM-FRET to the field of optical imaging and addresses their various applications, both current and potential, to anti-cancer drug delivery and cancer research.

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Section: Maximum Likelihood Analysismentioning

confidence: 99%

FLIM-FRET for Cancer Applications

Rajoria

Zhao

Intes

et al. 2015

CMI

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“…23 Though frequency-domain and time-domain approaches are in principle equivalent, being related to one another by a Fourier transform, there are tradeoffs between the two methods that determine which will be more appropriate to a given measurement scenario. 6,24 …”

Section: A Frequency-domain Lifetime Measurements With Lock-in Detecmentioning

confidence: 99%

Flexible digital signal processing architecture for narrowband and spread-spectrum lock-in detection in multiphoton microscopy and time-resolved spectroscopy

Wilson

Park

Warren

et al. 2015

Review of Scientific Instruments

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The lock-in amplifier is a critical component in many different types of experiments, because of its ability to reduce spurious or environmental noise components by restricting detection to a single frequency and phase. One example application is pump-probe microscopy, a multiphoton technique that leverages excited-state dynamics for imaging contrast. With this application in mind, we present here the design and implementation of a high-speed lock-in amplifier on the field-programmable gate array (FPGA) coprocessor of a data acquisition board. The most important advantage is the inherent ability to filter signals based on more complex modulation patterns. As an example, we use the flexibility of the FPGA approach to enable a novel pump-probe detection scheme based on spread-spectrum communications techniques. C 2015 AIP Publishing LLC. [http://dx

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“…In general, fluorescence measurements are performed by using an intensity-based approach (Bradley B. Collier & McShane, 2013). However, these intensity-based measurements are questionable due to fluorophore inhomogeneity, optoelectronic drift, photobleaching, or fluorescence quenching, all of which can result in concentration measurement inaccuracies (Bradley B. Collier & McShane, 2013;M.…”

Section: Introductionmentioning

confidence: 99%

Second-derivative laser-induced fluorescence spectroscopy combined with chemometrics for authentication of the adulteration of camellia oil

Chen

Geng

Chen

et al. 2018

CyTA - Journal of Food

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In this study, second-derivative laser-induced fluorescence spectroscopy (SD-LIF) and its derivative intrinsic-ratiometric laser-induced fluorescence spectroscopy (IR-LIF) were developed for the qualitative and quantitative analysis of camellia oil adulterated with sunflower oil or rapeseed oil, respectively. In the qualitative analysis, three pure oils and two blended oils were successfully discriminated based on SD-LIF and linear discriminant analysis. The influence of fluorescence concentration quenching on the adulterant concentration analysis was discussed. IR-LIF was established by using 646 nm chlorophyll b fluorescence as the insensitive reference and proved to be an effective approach to reduce the possible quenching influence on the full spectrum. Then, the combination of IR-LIF and partial least squares regression was employed to determine the adulterant concentration, and the results were satisfactory. The plots of the observed versus predicted adulteration concentration values showed high linearity. Root-mean-square errors of prediction lower than 3% were achieved. Espectroscopía de fluorescencia de segunda derivada inducida por láser combinada con quimiometría para la autenticación de la adulteración de aceite de camelia RESUMEN En el presente estudio se desarrollaron la espectroscopia de fluorescencia de segunda derivada inducida por láser (SD-LIF) y su derivada, la espectroscopia de fluorescencia intrínseca-ratiométrica inducida por láser (IR-LIF), para el análisis cualitativo y cuantitativo de aceite de camelia (CO) adulterado con aceite de girasol (SO) y con aceite de canola (RO), respectivamente. En el análisis cualitativo, se discriminaron con éxito tres aceites puros y dos aceites mezclados a partir de la SD-LIF y el análisis discriminante lineal (LDA). En este sentido, se analizó la influencia que tiene la desactivación de concentración de fluorescencia en el análisis de concentración del adulterante. Mediante el uso de 646 nm de fluorescencia de clorofila b como referencia insensible se estableció la IR-LIF, comprobándose que representa una opción efectiva para reducir la posible influencia de la desactivación en todo el espectro. Luego se utilizó la combinación de la IR-LIF y la regresión de mínimos cuadrados parciales (PLSR) para determinar la concentración del adulterante, obteniéndose resultados satisfactorios. Los diagramas de los valores de la concentración de adulteración observados versus los valores pronosticados muestran una alta linealidad. Se logró obtener raíces cuadradas de errores cuadráticos medios de predicción (RMSEP) inferiores a 3%.

show abstract

Time-resolved measurements of luminescence

Cited by 40 publications

References 106 publications

FLIM-FRET for Cancer Applications

FLIM-FRET for Cancer Applications

Flexible digital signal processing architecture for narrowband and spread-spectrum lock-in detection in multiphoton microscopy and time-resolved spectroscopy

Second-derivative laser-induced fluorescence spectroscopy combined with chemometrics for authentication of the adulteration of camellia oil

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