Time dependence of enzyme synergism during the degradation of model and natural lignocellulosic substrates

Malgas, Samkelo; Thoresen, Mariska; Dyk, J. Susan van; Pletschke, Brett I.

doi:10.1016/j.enzmictec.2017.04.007

Cited by 58 publications

(45 citation statements)

References 98 publications

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“…The degradation of cellulose to glucose involves the synergistic action of endo-β-1,4-glucanases, cellobiohydrolases, and β-glucosidases. This synergy can be expressed as synergy degree (SD), which is the ratio between the mixture activity and the sum of the individual cellulase activities [27,28]. The synergy can be explained by a model where endo-β-1,4-glucanases hydrolyze the interior of the cellulose polymer, generating new reducing ends for the action of the cellobiohydrolase (Figure 2) [29].…”

Section: Cellulases Synergismmentioning

confidence: 99%

“…The synergy can be explained by a model where endo-β-1,4-glucanases hydrolyze the interior of the cellulose polymer, generating new reducing ends for the action of the cellobiohydrolase (Figure 2) [29]. Although, this might be an oversimplification of cellulase synergy because there are other factors that influence cellulase synergy [27]. One factor is the ratio and concentration of the cellulases in the reaction mixture (e.g., in an endo-exo mixture, low ratios of the endoglucanase result in the strongest synergistic effect) [30].…”

Section: Cellulases Synergismmentioning

confidence: 99%

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Engineering Robust Cellulases for Tailored Lignocellulosic Degradation Cocktails

Contreras

Pramanik

Рожкова

et al. 2020

IJMS

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Lignocellulosic biomass is a most promising feedstock in the production of second-generation biofuels. Efficient degradation of lignocellulosic biomass requires a synergistic action of several cellulases and hemicellulases. Cellulases depolymerize cellulose, the main polymer of the lignocellulosic biomass, to its building blocks. The production of cellulase cocktails has been widely explored, however, there are still some main challenges that enzymes need to overcome in order to develop a sustainable production of bioethanol. The main challenges include low activity, product inhibition, and the need to perform fine-tuning of a cellulase cocktail for each type of biomass. Protein engineering and directed evolution are powerful technologies to improve enzyme properties such as increased activity, decreased product inhibition, increased thermal stability, improved performance in non-conventional media, and pH stability, which will lead to a production of more efficient cocktails. In this review, we focus on recent advances in cellulase cocktail production, its current challenges, protein engineering as an efficient strategy to engineer cellulases, and our view on future prospects in the generation of tailored cellulases for biofuel production.

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Section: Cellulases Synergismmentioning

confidence: 99%

Section: Cellulases Synergismmentioning

confidence: 99%

Engineering Robust Cellulases for Tailored Lignocellulosic Degradation Cocktails

Contreras

Pramanik

Рожкова

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…[14][15][16] Although many studies have been carried out on the synergistic hydrolysis of lignocellulosic biomass by cellulase and xylanse, the mechanism behind this synergistic effect during the hydrolysis of pretreated lignocellulosic biomass was still unclear, obviously preventing further development of this technology. [17][18][19][20] In this work, sugarcane bagasse (SCB) substrates with different chemical compositions were prepared by different pretreatments including dilute acid (DA), acidic sodium chlorite (ASC), alkali solution (AS), and alkali hydrogen peroxide (AHP). The compositions and chemical structures of untreated and pretreated SCB were characterized by HPLC, FTIR, XRD, and SEM.…”

Section: Introductionmentioning

confidence: 99%

Comparison of different pretreatments on the synergistic effect of cellulase and xylanase during the enzymatic hydrolysis of sugarcane bagasse

Huang

Zhao

et al. 2018

RSC Adv.

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“…Furthermore, the immobilized cellulase is more resistant to structural alterations induced by increased temperature [36][37][38][39]. The activity of bounded cellulase was shown to be higher at most pH values than the free form due to enhanced stability [40][41][42].…”

Section: Introductionmentioning

confidence: 99%

Recent Advances of Cellulase Immobilization onto Magnetic Nanoparticles: An Update Review

et al. 2019

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Cellulosic enzymes, including cellulase, play an important role in biotechnological processes in the fields of food, cosmetics, detergents, pulp, paper, and related industries. Low thermal and storage stability of cellulase, presence of impurities, enzyme leakage, and reusability pose great challenges in all these processes. These challenges can be overcome via enzyme immobilization methods. In recent years, cellulase immobilization onto nanomaterials became the focus of research attention owing to the surface features of these materials. However, the application of these nanomaterials is limited due to the efficacy of their recovery process. The application of magnetic nanoparticles (MNPs) was suggested as a solution to this problem since they can be easily removed from the reaction mixture by applying an external magnet. Recently, MNPs were extensively employed for enzyme immobilization owing to their low toxicity and various practical advantages. In the present review, recent advances in cellulase immobilization onto functionalized MNPs is summarized. Finally, we discuss enhanced enzyme reusability, activity, and stability, as well as improved enzyme recovery. Enzyme immobilization techniques offer promising potential for industrial applications.

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Time dependence of enzyme synergism during the degradation of model and natural lignocellulosic substrates

Cited by 58 publications

References 98 publications

Engineering Robust Cellulases for Tailored Lignocellulosic Degradation Cocktails

Engineering Robust Cellulases for Tailored Lignocellulosic Degradation Cocktails

Comparison of different pretreatments on the synergistic effect of cellulase and xylanase during the enzymatic hydrolysis of sugarcane bagasse

Recent Advances of Cellulase Immobilization onto Magnetic Nanoparticles: An Update Review

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