Comparison of different pretreatments on the synergistic effect of cellulase and xylanase during the enzymatic hydrolysis of sugarcane bagasse

Huang, Chao; Zhao, Cheng; Li, Hailong; Chen, Xuefang; Luo, Mutan; Chen, Xinde

doi:10.1039/c8ra05047c

Cited by 19 publications

(9 citation statements)

References 30 publications

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“…The fiber purification process consisted of an alkali treatment that solubilized the lignin, pectins, hemicelluloses, proteins, and low-molecular weight sugars followed by a bleaching process that removed the lignin residues . The removal of part of the hemicellulose and lignin fractions is required in order to facilitate the enzymatic hydrolysis reactions, since these components constitute a natural cell wall barrier to the cellulose fibers. , A linear relationship between lignin removal and cellulose digestibility by enzymatic action has been demonstrated previously. ,, …”

Section: Resultsmentioning

confidence: 95%

“…The purification procedure was necessary to ensure an efficient removal of noncellulosic materials, especially lignin, which hinder the access of enzymes to the cellulose, impairing the viability of enzymatic hydrolysis. 24 The methodology used followed a procedure adapted from Flauzino Neto et al 25 Briefly, 5 g of fibers were treated in 1 L of a 10% (w/w) sodium hydroxide aqueous solution for 1 h at 80 °C, under mechanical stirring, washed with distilled water until neutral pH, and then dried at 50 °C for 24 h. After this alkali treatment, the fibers were bleached with a solution made up of equal parts (v/v) of acetate buffer (27 wt % NaOH and 7.5% (v/v) glacial acetic acid, in distilled water) and aqueous sodium chlorite (1.7 wt % NaClO 2 in water). The proportion used in this bleaching treatment was 1 g of fiber per 50 mL of solution.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

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Enzymatic Deconstruction of Sugarcane Bagasse and Straw to Obtain Cellulose Nanomaterials

Aguiar

Bondancia

Claro

et al. 2020

ACS Sustainable Chem. Eng.

122

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The application of green process engineering strategies to obtain high-value, eco-friendly, and biodegradable materials from residual lignocellulosic biomass can contribute to the sustainability of future biorefineries. Here, we investigated the potential of sugarcane bagasse and straw as lignocellulosic biomass feedstocks to obtain nanocellulose, using the enzymatic hydrolysis route as a platform. The fibers were submitted to a purification process followed by enzymatic hydrolysis with a commercial enzyme cocktail. The cellulose nanomaterials obtained from both fibers (sugarcane bagasse and straw) presented a high crystallinity index (∼70%) and thermal stability (degradation onset temperatures higher than 300 °C). After the enzymatic hydrolysis, a centrifugation step was used to separate the cellulose nanocrystals (CNC) present in the nanocellulose samples. The increase of the duration of enzymatic hydrolysis resulted in an increase of the CNC content and decreases in the diameter and length of the CNC. These findings indicated the potential of using the enzymatic route as a platform to obtain nanocellulose as a value-added bioproduct from both sugarcane bagasse and straw. In addition to being eco-friendly, this process also releases a stream rich in soluble sugars that can be used to produce ethanol or other biobased products, within the biorefinery concept.

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Section: Resultsmentioning

confidence: 95%

Section: ■ Experimental Sectionmentioning

confidence: 99%

Enzymatic Deconstruction of Sugarcane Bagasse and Straw to Obtain Cellulose Nanomaterials

Aguiar

Bondancia

Claro

et al. 2020

ACS Sustainable Chem. Eng.

122

View full text Add to dashboard Cite

show abstract

“…was observed during the initial hydrolysis stage but it decreased in the later hydrolysis stage (Huang et al, 2018). In addition, it was observed that the digestibility of cellulase was increased with the digestibility of xylan in the hydrolysis of pretreated sugarcane bagasse substrates (Huang et al, 2018). Similarly, supplementing commercial cellulase (Sigma-Aldrich, USA) with BGL was compared for the hydrolysis of microcrystalline cellulase, native corn straw and steam pretreated corn straw and the results showed an increase in glucose release by 62, 94, and 95%, respectively (Zhang et al, 2017).…”

Section: Synergistic Enzyme Cooperationmentioning

confidence: 88%

Section: Synergistic Enzyme Cooperationmentioning

confidence: 98%

Synergistic Enzyme Cocktail to Enhance Hydrolysis of Steam Exploded Wheat Straw at Pilot Scale

et al. 2018

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Multiple enzymes are required for efficient hydrolysis of lignocellulosic biomass and no wild type organism is capable of producing all enzymes in desired levels. In this study, steam explosion of wheat straw was carried out at pilot scale and a synthetic enzyme mixture (EnzMix) was developed by partially replacing the cellulase with critical dosages of commercially available accessory enzymes (β-glucosidase, xylanase and laccase) through central composite design. Highest degree of synergism (DS) was observed with β-glucosidase (1.68) followed by xylanase (1.36). Finally, benchmarking of EnzMix (Celluclast, β-glucosidase and xylanase in a protein ratio of 20.40: 38.43: 41.16, respectively) and other leading commercial enzymes was carried out. Interestingly, hydrolysis improved by 75% at 6 h and 30% at 24 h, respectively in comparison of control. By this approach, 25% reduction in enzyme dosage was observed for obtaining the same hydrolysis yield with opitimized enzyme cocktail. Thus, development of enzyme cocktail is an effective and sustainable approach for high hydrolysis efficiency.

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“…Similarly, the xylanase family 10 Xyn10Z responsible for xylan solubilization was frequently chosen for artificial cellulosomes [6,22]. The cellulase-xylanase synergy that facilitates the decomposition of complex substrates was noticed in previous studies [16,[25][26][27][28][29]. In addition, the saccharolytic enzymes originated from the aerobic thermophilic bacterium Thermobifida fusca, with thermostability and potent catalytic efficiency [30], are identified as important starting materials for constructing DCs in several studies [16,29,[31][32][33].…”

Section: Integration Of Laccase Into Designer Cellulosomesmentioning

confidence: 99%

Clostridium thermocellum as a Promising Source of Genetic Material for Designer Cellulosomes: An Overview

2021

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Plant biomass-based biofuels have gradually substituted for conventional energy sources thanks to their obvious advantages, such as renewability, huge quantity, wide availability, economic feasibility, and sustainability. However, to make use of the large amount of carbon sources stored in the plant cell wall, robust cellulolytic microorganisms are highly demanded to efficiently disintegrate the recalcitrant intertwined cellulose fibers to release fermentable sugars for microbial conversion. The Gram-positive, thermophilic, cellulolytic bacterium Clostridium thermocellum possesses a cellulolytic multienzyme complex termed the cellulosome, which has been widely considered to be nature’s finest cellulolytic machinery, fascinating scientists as an auspicious source of saccharolytic enzymes for biomass-based biofuel production. Owing to the supra-modular characteristics of the C. thermocellum cellulosome architecture, the cellulosomal components, including cohesin, dockerin, scaffoldin protein, and the plentiful cellulolytic and hemicellulolytic enzymes have been widely used for constructing artificial cellulosomes for basic studies and industrial applications. In addition, as the well-known microbial workhorses are naïve to biomass deconstruction, several research groups have sought to transform them from non-cellulolytic microbes into consolidated bioprocessing-enabling microbes. This review aims to update and discuss the current progress in these mentioned issues, point out their limitations, and suggest some future directions.

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Comparison of different pretreatments on the synergistic effect of cellulase and xylanase during the enzymatic hydrolysis of sugarcane bagasse

Abstract: The synergistic effects between cellulase and xylanase were substrate and time specific during the hydrolysis of pretreated SCB.

Cited by 19 publications

References 30 publications

Enzymatic Deconstruction of Sugarcane Bagasse and Straw to Obtain Cellulose Nanomaterials

Enzymatic Deconstruction of Sugarcane Bagasse and Straw to Obtain Cellulose Nanomaterials

Synergistic Enzyme Cocktail to Enhance Hydrolysis of Steam Exploded Wheat Straw at Pilot Scale

Clostridium thermocellum as a Promising Source of Genetic Material for Designer Cellulosomes: An Overview

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