Time-Course Statistical Evaluation of Intercellular Adhesion Maturation by Femtosecond Laser Impulse

Iino, Takanori; Hagiyama, Man; Furuno, Tadahide; Ito, Akihiko; Hosokawa, Yoichiroh

doi:10.1016/j.bpj.2016.09.044

Cited by 13 publications

(7 citation statements)

References 41 publications

(49 reference statements)

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“…Previously, we developed a method using a femtosecond laser to generate impulsive force, allowing quantification of cell adhesion between cultured cells (e.g. leukocytes and endothelial cells), epithelial cells with one another, and neurons and mast cells ( Hosokawa et al, 2011 ; Iino et al, 2016 ; Kaji et al, 2007 ). When a femtosecond laser is focused through an objective lens, a stress wave is generated at the laser focal point.…”

Section: Introductionmentioning

confidence: 99%

Quantitative analysis of mechanical force required for cell extrusion in zebrafish embryonic epithelia

et al. 2017

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When cells in epithelial sheets are damaged by intrinsic or extrinsic causes, they are eliminated by extrusion from the sheet. Cell extrusion, which is required for maintenance of tissue integrity, is the consequence of contraction of actomyosin rings, as demonstrated by both molecular/cellular biological experimentation and numerical simulation. However, quantitative evaluation of actomyosin contraction has not been performed because of the lack of a suitable direct measurement system. In this study, we developed a new method using a femtosecond laser to quantify the contraction force of the actomyosin ring during cell extrusion in zebrafish embryonic epithelia. In this system, an epithelial cell in zebrafish embryo is first damaged by direct femtosecond laser irradiation. Next, a femtosecond laser-induced impulsive force is loaded onto the actomyosin ring, and the contraction force is quantified to be on the order of kPa as a unit of pressure. We found that cell extrusion was delayed when the contraction force was slightly attenuated, suggesting that a relatively small force is sufficient to drive cell extrusion. Thus, our method is suitable for the relative quantitative evaluation of mechanical dynamics in the process of cell extrusion, and in principle the method is applicable to similar phenomena in different tissues and organs of various species.

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Section: Introductionmentioning

confidence: 99%

Quantitative analysis of mechanical force required for cell extrusion in zebrafish embryonic epithelia

et al. 2017

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“…Thus, other calculations, such as measuring adhesion strength, are necessary to better understand these metrics for the shipping system. Indeed, there have been reports that the adhesion strength between cells can be measured using laser impact (Hosokawa, Hagiyama, Iino, Murakami, & Ito, ; Iino, Hagiyama, Furuno, Ito, & Hosokawa, ). In these reports, the force of micronewton in a microsecond timescale was necessary to cleave cell–cell adhesion.…”

Section: Discussionmentioning

confidence: 99%

A simple and static preservation system for shipping retinal pigment epithelium cell sheets

Hori

Kuwabara²,

Tanaka

et al. 2019

J Tissue Eng Regen Med

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433 Effect of the small compound TD -198946 on glycosaminoglycan synthesis and transforming growth factor β 3-associated chondrogenesis of human synovium-derived stem cells in vitro A simple and static preservation system for shipping retinal pigment epithelium cell sheets K. Hori, J. Kuwabara, Y. Tanaka, M. Nishida, N. Koide and M. Takahashi 459 DNA methyltransferases inhibitors effectively induce gene expression changes suggestive of cardiomyogenic differentiation of human amniotic fluid-derived mesenchymal stem cells via chromatin remodeling M. Gasiūnienė, A. Zentelytė, B. Wojtas, S. Baronaitė, N. Krasovskaja, J. Savickienė, B. Gielniewski, B. Kaminska, A. Utkus and R. Navakauskienė 469 Differentiation of the human liver progenitor cell line (HepaRG) on a microfluidic-based biochip M. Jang, A. Kleber, T. Ruckelshausen, R. Betzholz and A. Manz 482 A magnetic three-dimensional levitated primary cell culture system for the development of secretory salivary gland-like organoids The cover image is based on the Research Article A simple and static preservation system for shipping retinal pigment epithelium cell sheets by Kanji Hori et al., https://doi.org/10.1002/term.2805. © iP-TEC® Premier BOX-V8.5/ iP-TEC® Temperature Stabilizer-24. Reproduced with permission by

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“…These data suggest that a decrease in the adhesion force at the cell body is important to propel somal translocation ( Figure 3B ). Although this assay is qualitative, other recent studies have reported a quantitative cell detachment assay: using femtosecond lasers combined with atomic force microscopy (AFM), controlled impulsive forces to induce cell detachment can be applied to estimate the adhesion force of cells ( Hosokawa et al, 2011 ; Iino et al, 2016 ). In addition, cell adhesion molecules tagged with pH-sensitive GFP could enable analyses of spatiotemporal dynamics of adhesions in migrating neurons ( Famulski et al, 2010 ).…”

Section: Forces For Somal Translocationmentioning

confidence: 99%

Forces to Drive Neuronal Migration Steps

Minegishi

Inagaki

2020

Front. Cell Dev. Biol.

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To establish and maintain proper brain architecture and elaborate neural networks, neurons undergo massive migration. As a unique feature of their migration, neurons move in a saltatory manner by repeating two distinct steps: extension of the leading process and translocation of the cell body. Neurons must therefore generate forces to extend the leading process as well as to translocate the cell body. In addition, neurons need to switch these forces alternately in order to orchestrate their saltatory movement. Recent studies with mechanobiological analyses, including traction force microscopy, cell detachment analyses, live-cell imaging, and loss-of-function analyses, have begun to reveal the forces required for these steps and the molecular mechanics underlying them. Spatiotemporally organized forces produced between cells and their extracellular environment, as well as forces produced within cells, play pivotal roles to drive these neuronal migration steps. Traction force produced by the leading process growth cone extends the leading processes. On the other hand, mechanical tension of the leading process, together with reduction in the adhesion force at the rear and the forces to drive nucleokinesis, translocates the cell body. Traction forces are generated by mechanical coupling between actin filament retrograde flow and the extracellular environment through clutch and adhesion molecules. Forces generated by actomyosin and dynein contribute to the nucleokinesis. In addition to the forces generated in cell-intrinsic manners, external forces provided by neighboring migratory cells coordinate cell movement during collective migration. Here, we review our current understanding of the forces that drive neuronal migration steps and describe the molecular machineries that generate these forces for neuronal migration.

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Time-Course Statistical Evaluation of Intercellular Adhesion Maturation by Femtosecond Laser Impulse

Cited by 13 publications

References 41 publications

Quantitative analysis of mechanical force required for cell extrusion in zebrafish embryonic epithelia

Quantitative analysis of mechanical force required for cell extrusion in zebrafish embryonic epithelia

A simple and static preservation system for shipping retinal pigment epithelium cell sheets

Forces to Drive Neuronal Migration Steps

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