Regenerative medicine has received a lot of attention as a novel strategy for injuries and diseases that are difficult to cure using current techniques. Cell production, which is vital for regenerative medicine, has undergone remarkable progress via breakthroughs in developmental biology and tissue engineering; currently, cell production requires numerous experimental operators performing manual, small-scale cell cultures. Other major obstacles for cell production and regenerative medicine include the variable quality of products based on the experimental procedure, the skills of operators, the level of labor required for production, and costs. Technological developments are required to overcome this, including automation instead of manual culture. Age-related macular regeneration (AMD) is a refractory ocular disease that causes severe deterioration in central vision due to senescence in the retinal pigment epithelium (RPE). Recently, we performed an autologous transplantation of induced pluripotent stem (iPS) cell-derived RPE cell sheets and started clinical research on allografts from RPE cell suspensions differentiated from iPS cells. The use of regenerative therapies for AMD using iPS cell-derived RPE is expected to become more widespread. In the present study, human iPS cell-derived RPE cells were cultured to form RPE cell sheets using equipment with a closed culture module. The quality of the automated cultured RPE cell sheets was confirmed by comparing their morphological and biological properties with those of manually generated RPE cell sheets. As a result, machine-cultured RPE sheets displayed the same quality as manually cultured RPE sheets, showing that iPS cell-derived RPE cell sheets were successfully cultured by an automated process.
433 Effect of the small compound TD -198946 on glycosaminoglycan synthesis and transforming growth factor β 3-associated chondrogenesis of human synovium-derived stem cells in vitro A simple and static preservation system for shipping retinal pigment epithelium cell sheets K. Hori, J. Kuwabara, Y. Tanaka, M. Nishida, N. Koide and M. Takahashi 459 DNA methyltransferases inhibitors effectively induce gene expression changes suggestive of cardiomyogenic differentiation of human amniotic fluid-derived mesenchymal stem cells via chromatin remodeling M. Gasiūnienė, A. Zentelytė, B. Wojtas, S. Baronaitė, N. Krasovskaja, J. Savickienė, B. Gielniewski, B. Kaminska, A. Utkus and R. Navakauskienė 469 Differentiation of the human liver progenitor cell line (HepaRG) on a microfluidic-based biochip M. Jang, A. Kleber, T. Ruckelshausen, R. Betzholz and A. Manz 482 A magnetic three-dimensional levitated primary cell culture system for the development of secretory salivary gland-like organoids The cover image is based on the Research Article A simple and static preservation system for shipping retinal pigment epithelium cell sheets by Kanji Hori et al., https://doi.org/10.1002/term.2805. © iP-TEC® Premier BOX-V8.5/ iP-TEC® Temperature Stabilizer-24. Reproduced with permission by
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